Developmental Stages in the Rabbit Embryo: Guidelines to Choose an Appropriate Experimental Model

Beaudoın, Sylvie; Barbet, P.; Bargy, F.

doi:10.1159/000073136

Cited by 50 publications

(55 citation statements)

References 17 publications

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“…De acordo com Beaudoin et al (2003), a embriogênese de algumas espécies está correlacionada com uma série de mudanças contínuas, de uma maneira similar a que ocorre no desenvolvimento pré-natal nas diferentes espécies. Em geral, a descrição do desenvolvimento embrionário e fetal em outras espécies foi baseada em estágios construídos através da idade, mudanças corporais por diferenciação gradual, aumento do tamanho do corpo e peso, e desenvolvimento total de sistemas e órgãos (Knospe 2002, Beaudoin et al 2003, Ambrósio et al 2002, Miglino et al 2006.…”

Section: Discussionunclassified

Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais

et al. 2011

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Pesq. Vet. Bras. 31(Supl. The study of the embryology of the domestic cat is of great value considering its importance as an experimental model for the wild cats endangered from extinction, especially in the research related to reproductive biology. The objective of this study is the descriptive embryology of the domestic cat at different stages of pregnancy, through macroscopic description of photographic records, radiographic and alizarin technique, and microscopic description of photographic records by light microscopy. In embryos with an estimated gestational age of 17 days we observed macroscopically an expansion corresponding to the rostral forebrain, the placoid site of lens, cervical ϐlexure, the four pharyngeal arches with grooves dividing the cardiac prominence, a sign of the limb bud, and the presence of somites. In the caudal region of the embryo, we saw the cranio-caudal bend, allowing the same position in format of a "C". In embryos with an estimated age of 22 days, we noticed macroscopically the forebrain, optic vesicle pigmentation of the retina, the optic vesicle, fourth ventricle, liver, fore and hind limbs with a slight distinction between the digits and superϐicial vascularization. In embryos with an estimated age of 25 days we noticed presence of the forebrain and midbrain, the pronounced cervical curvature of the optic vesicle with strong pigmentation of the retina, the optic vesicle, limbs and chest well developed, distinguishing the digits and pronounced the liver Fetuses with estimated age of 52 days have internal and external structures easily identiϐied in adult animals. With respect to the bone structure we noted that they did not have any radial bone formed, only bone shafts. Microscopically, the embryo of the domestic cat with CR of 0.9cm and estimated age of 19 days revealed the presence of beak, oral cavity with upper and lower nasal cavity, eye and opening of the 4 th ventricle of the brain, esophagus, heart with atrium and ventricle, lung, liver, mesonephric ridge, primitive gonad, stomach,

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Section: Discussionunclassified

Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais

et al. 2011

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“…Laparotomies were performed under anesthesia at day 17 (E17) or day 21 (E21) of gestation (gestation = 31-32 days) as described previously [20,21]. The fetus was located and fixed by external palpation of the semitransparent uterine wall, and intra-amniotic or intrahepatic injections were performed using 26-gauge needles in view of the common anatomical landmarks of the E15-22 rabbit embryos [26]. To ensure intrahepatic injection, the fetus was punctured at the lower level of the thorax.…”

Section: Animals and Transplantation Proceduresmentioning

confidence: 99%

“…The fetuses administered at gestation day 17 received a unique 100 mL dose of 3.5 · 10 5 fl-MSC-EGFP + cells, whereas those administered at gestation day 21 received a unique and comparable statural growth-adjusted 100 mL dose of 5 · 10 5 fl-MSC-EGFP + cells [26]. Control fetuses were injected with an identical volume of PBS.…”

Section: Animals and Transplantation Proceduresmentioning

confidence: 99%

Fetal Liver-Derived Mesenchymal Stem Cell Engraftment After Allogeneic In Utero Transplantation into Rabbits

Moreno

Martínez-González²,

Rosal³

et al. 2012

Stem Cells and Development

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Prenatal transplantation of genetically engineered mesenchymal stem cells (MSCs) might benefit prevention or treatment of early-onset genetic disorders due to the cells' intrinsic regenerative potential plus the acquired advantage from therapeutic transgene expression. However, a thorough assessment of the safety, accessibility, and behavior of these MSCs in the fetal environment using appropriate animal models is required before we can advance toward a clinical application. We have recently shown that fetal rabbit liver MSCs (fl-MSCs) have superior growth rate, clonogenic capability, and in vitro adherence and differentiation abilities compared with adult rabbit bone marrow MSCs. In this follow-up study, we report safe and widespread distribution of recombinant pSF-EGFP retrovirus-transduced fl-MSCs (EGFP + -fl-MSCs) in neonatal rabbit tissues at 10 days after fetal allogeneic transplantation through both intrahepatic and intra-amniotic administration. Conversely, a more restricted biodistribution pattern according to the route of administration was apparent in the young rabbits intervened at 16 weeks after fetal EGFP + -fl-MSC transplantation. Furthermore, the presence of these cells in the recipients' tissues, tracked with the reporter provirus, was inversely related to the developmental stage of the fetuses at the time of intervention. Long-term engraftment was confirmed both by fluorescence in situ hybridization analysis on touch tissue imprints using a chromosome Y-specific BAC probe, and by immunohistochemical localization of EGFP expression. Finally, there was no evidence of immune responses against the transplanted EGFP + -fl-MSCs or the EGFP transgenic product in the treated young rabbits. Thus, cell transplantation approaches using genetically engineered fetal MSCs may prove particularly valuable to frontier medical treatments for congenital birth defects in perinatology.

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“…Embryogenesis of a given specimen in any species correlates with a series of continuous changes in a similar manner that prenatal development occurs in the different species (Knospe, 2002;Beaudoin et al, 2003). In spite of accepted similarities, there is a number of embryo classifying systems.…”

Section: Discussionmentioning

confidence: 99%

External Morphological Characterization of 60-Days Gestation Myocastor coypus (Coypu) Fetuses

et al. 2006

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SUMMARY:The main objective of this work was to carry out the external morphology characterization of 60-days gestation Myocastor coypus (coypu) fetuses since this stage could be considered a crucial point in the prenatal development in this species. Fourteen fetuses out of three litter were analised. Gestational sacs weighing 3.81 ± 0.27 g showed an elliptical shape with the larger axis along the direction of the uterus. The average weight of fetuses was 1.13 ± 0.19 g, the head-to-tail lenght was 1.99 ± 0.87 cm, the cephalic lenght was 1.01 ± 0.37 cm, and the biparietal diameter was 0.63 ± 0.06 cm. Specimens showed differentiation in corporeal, limbs and tail regions; also showed interdigital membranes, well defined optical vesicles and lateral ear lobe sprouts. Comparative analysis of coypu fetuses and equivalent stages of other mammal species will allow to establish similitudes and differences that would permit to mark particularities in the ontogeny of Myocastor coypus.

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Developmental Stages in the Rabbit Embryo: Guidelines to Choose an Appropriate Experimental Model

Cited by 50 publications

References 17 publications

Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais

Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus) em diferentes idades gestacionais

Fetal Liver-Derived Mesenchymal Stem Cell Engraftment After Allogeneic In Utero Transplantation into Rabbits

External Morphological Characterization of 60-Days Gestation Myocastor coypus (Coypu) Fetuses

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