Developmental stages, incubation temperature, and in vivo traceability of primordial germ cell in an important aquaculture species Piaractus mesopotamicus

Coelho, Geovanna Carla Zacheo; Arashiro, Dilberto Ribeiro; Disselli, Tamiris; Pereira-Santos, Matheus; Mira-López, Tatiana M.; Monzani, Paulo Sérgio; Senhorini, José Augusto; Fujimoto, Takafumi; Yasui, George Shigueki

doi:10.1016/j.aquaculture.2021.736381

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…The yellowtail tetra is being used as a model for other endangered characin species, such as Brycon orbignyanus . Other aquaculture characins, such as the streaked prochilod ( Prochilodus lineatus ) and pacu ( Piaractus mesopotamicus ) ( Coelho et al, 2019 , 2021 , respectively), are also being studied to serve as cell donors, using yellowtail tetra to produce gametes from those species. As the sterile host was already established ( Piva et al, 2018 ), transplantation procedures are now being established for several characin species.…”

Section: Surrogate Propagation Using Yellowtail Tetramentioning

confidence: 99%

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

et al. 2022

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The use of model organisms is important for basic and applied sciences. Several laboratory species of fishes are used to develop advanced technologies, such as the zebrafish (Danio rerio), the medaka (Oryzias latipes), and loach species (Misgurnus spp.). However, the application of these exotic species in the Neotropical region is limited due to differences in environmental conditions and phylogenetic distances. This situation emphasizes the establishment of a model organism specifically for the Neotropical region with the development of techniques that may be applicable to other Neotropical fish species. In this work, the previous research efforts are described in order to establish the yellowtail tetra Astyanax altiparanae as a model laboratory species for both laboratory and aquaculture purposes. Over the last decade, starting with artificial fertilization, the yellowtail tetra has become a laboratory organism for advanced biotechnology, such as germ cell transplantation, chromosome set manipulation, and other technologies, with applications in aquaculture and conservation of genetic resources. Nowadays, the yellowtail tetra is considered the most advanced fish with respect to fish biotechnology within the Neotropical region. The techniques developed for this species are being used in other related species, especially within the characins class.

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Section: Surrogate Propagation Using Yellowtail Tetramentioning

confidence: 99%

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

et al. 2022

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“…The importance of PGCs as germplasm preservation material includes their cellular pluripotency characteristics, as they are the rst lineages of germ cells that migrate to the gonadal ridge during embryonic development and participate in the formation of gonads (Blaser et al 2005 The PGCs could be labeled in vivo using synthetic mRNAs. The 3'UTR sequences of PGC-speci c mRNAs from one species are used to identify PGCs of other species (Saito et al 2006;Coelho et al 2019Coelho et al , 2021. It is possible due to 3'UTR sequences sharing similarity, being observed conserved sequences between species (Xiao et al 2019;Zhou et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Traceability of PGCsMicromanipulation was performed according toCoelho et al (2021). The gfp-Pm-ddx4 3'UTR and dsred-Pm-ddx4 3'UTR mRNAs microinjection was achieved in the zygotes of P. mangurus, A. altiparane, and P. lineatus.…”

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Characterization of the migratory pattern of Primordial Germ Cells in three neotropical fish species aiming genetic conservation actions

Rosero,

Monzani,

Pessoa

et al. 2023

Preprint

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Primordial germ cells (PGCs) are embryonic pluripotent cells that can differentiate into spermatogonia and oogonia, making them a genetic source for germplasm conservation through cryobanking and the generation of germline chimeras. Therefore, PGC studies should be conducted for each species, given possible divergences in migratory patterns of PGCs. In this work, the PGCs of 3 neotropical species (Pseudopimelodus mangurus, Astyanax altiparanae, and Prochilodus lineatus) were characterized by microinjection into zygotes of mRNA synthesized from the ddx4 3'UTR sequence of P. mangurus. Visualization of labeled PGCs was achieved by fluorescence microscopy during embryonic development. In addition, ddx4 and dnd1 expressions were evaluated during embryonic development, larvae, and adult tissues of P. mangurus, to validate their use as a PGC marker. As a result, the effective identification of PGCs was obtained. DsRed-positive PGC of P. mangurus was observed in the hatching stage, GFP-positive PGC of A. altiparanae in the gastrula stage, and GFP-positive PGCs from P. lineatus were identified at the segmentation stage, with representative labeling percentages of 29% and 16% in A. altiparanae and P. lineatus respectively. The expression of ddx4 and dnd1 of P. mangurus confirmed the specificity of these genes in germ cells. These results point to the functionality of the P. mangurus ddx4 3'UTR sequence as a PGC marker, demonstrating that PGC labeling was more efficient in A. altiparanae and P. lineatus. The procedures used to identify PGCs in P. mangurus consolidate the first step for generating germinal chimeras as a conservation action of P. mangurus.

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Traceability of primordial germ cells in three neotropical fish species aiming genetic conservation actions

Rosero,

Monzani,

Pessoa

et al. 2023

Fish Physiol Biochem

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Developmental stages, incubation temperature, and in vivo traceability of primordial germ cell in an important aquaculture species Piaractus mesopotamicus

Cited by 4 publications

References 29 publications

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

Characterization of the migratory pattern of Primordial Germ Cells in three neotropical fish species aiming genetic conservation actions

Traceability of primordial germ cells in three neotropical fish species aiming genetic conservation actions

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