Developmentally regulated expression of Thy-1 in structures of the mouse sensory-motor system

Abstract

Thy‐1 is a cell‐surface molecule of the immunoglobulin superfamily which is expressed at high levels in the mature nervous system. Thy‐1 has been implicated in regulating axonal outgrowth and synaptic function, but little is known regarding its cellular localization and expression in the central nervous system (CNS) during development or in adulthood. In this study, Thy‐1 gene expression and protein localization were examined in sensory‐motor and related areas of the adult and postnatally developing mouse CNS. Thy‐1 mRNA expression was restricted to neurons; immunoreactivity was densely distributed throughout the neuropil of all regions examined, often delineated the neuronal plasmalemma, and labeled axons in white matter tracts of the brain and spinal cord. In adulthood, immunolabeling was regionally widespread and was present relatively homogeneously throughout all cell‐dense layers of sensory‐motor cortex, throughout most thalamic nuclei, globus pallidus, and spinal cord. Developmentally, however, Thy‐1 expression and localization exhibited a spatially and temporally staggered sequence leading to the adult pattern. In sensory‐motor cortex, Thy‐1 expression in layer V preceded expression in other layers; in the barrel field, labeling of barrel septa preceeded a gradually increasing intensity of immunolabeling of barrel centers; in the thalamus, Thy‐1 exhibited a differential onset and temporal pattern of expression across different nuclei associated with motor, sensory, or limbic systems; in the caudate nucleus, Thy‐1 expression was greatest during the first postnatal week of life before declining during subsequent development. Taken together, the adult distribution and developmental patterns leading to it form a unique profile in comparison with other structurally related glycosyl‐phosphatidylinositol (GPI)‐anchored neural cell adhesion molecules. The pattern and timing of Thy‐1 expression across layers and nuclei during early postnatal development are more complex than previously recognized, thus perhaps reflecting varied roles for Thy‐1 in aspects of structural or functional maturation which proceed independently of the timing of neurogenesis, migration, and dendritic and axonal growth. J. Comp. Neurol. 421:215–233, 2000. © 2000 Wiley‐Liss, Inc.

Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

“…The surface protein Thy-1 is enriched in neurons throughout the CNS [40,41] and binds αvβ5 integrin [25], but its role in the brain is unknown. Primary cortical neurons were incubated with Thy-1 blocking or IgG control antibodies prior to seeding onto primary astrocyte monolayers.…”

Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

“…CD90, also known as Thy-1, is expressed on hematopoietic cells (Imbert et al, 1998;Murray et al, 1999a;Stewart et al, 2000), thymic nurse cells (Bodey et al, 1999), neuronal tissue (Barlow and Huntley, 2000), renal mesangial cells (Tamura et al, 2000), placenta (Bukovsky et al, 1999) and some connective tissues (Saalbach et al, 1999). Human peripheral blood cells positive for both CD90 and CD34 include hematopoietic stem cells capable of producing multiple hematopoietic lineages (Murray et al, 1999a,b;Stewart et al, 2000).…”

Preparation of water‐soluble chitosan derivatives and their antibacterial activity

“…For each age, at least four to six animals were used per probe. Sections were processed for localization of radioactive cRNA probes as described previously (Barlow and Huntley, 2000). Briefly, slide‐mounted sections were washed in 0.1 M glycine and then pretreated with proteinase K (1 μg/ml; pH 8), 0.25% acetic anhydride in 0.1 M triethanolamine, and two washes of 2× saline sodium citrate (SSC).…”