Developments in nitrile and amide biotransformation processes

Velankar, Harshad Ravindra; Clarke, Kim Gail; Preez, Ryne du; Cowan, Don A.; Burton, Stephanie G.

doi:10.1016/j.tibtech.2010.08.004

Cited by 52 publications

(23 citation statements)

References 88 publications

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“…The activity of immobilized lipase in sol–gel was enhanced by up to 100-fold compared with the free ones [124]. Moreover, immobilization improved the operational stability, and immobilized cells were found to hydrolyze a wider range of nitriles than free cells [125]. Hydrolysis may have contributed to the protective role of immobilized cells against the toxic effect of nitrile substrates.…”

Section: Introductionmentioning

confidence: 99%

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

et al. 2012

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Over the past decades, nitrilases have drawn considerable attention because of their application in nitrile degradation as prominent biocatalysts. Nitrilases are derived from bacteria, filamentous fungi, yeasts, and plants. In-depth investigations on their natural sources function mechanisms, enzyme structure, screening pathways, and biocatalytic properties have been conducted. Moreover, the immobilization, purification, gene cloning and modifications of nitrilase have been dwelt upon. Some nitrilases are used commercially as biofactories for carboxylic acids production, waste treatment, and surface modification. This critical review summarizes the current status of nitrilase research, and discusses a number of challenges and significant attempts in its further development. Nitrilase is a significant and promising biocatalyst for catalytic applications.

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Section: Introductionmentioning

confidence: 99%

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

et al. 2012

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“…High substrate concentrations, biocatalyst stability, and reuse through immobilization strategies provide driving forces towards more efficient productivity. Moreover, the combined use of organic solvents and immobilized biocatalysts, including the selection of an appropriate medium, could be applied to this biotransformation technique and improve the yield of 5‐CVAM 16, 17…”

Section: Resultsmentioning

confidence: 99%

Highly efficient synthesis of 5‐cyanovaleramide by Rhodococcus ruber CGMCC3090 resting cells

Shen

Zhang

et al. 2012

J of Chemical Tech & Biotech

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“…Retention and reuse of the isolated enzymes by immobilisation in or on a matrix provide further benefit by facilitating continuous operation strategies. Membrane immobilisation has been specifically highlighted as a route to maximise process performance [4]. Membrane immobilisation techniques include ultrafiltration membranes where the enzyme is immobilised in or on the pores while the product, with a lower molecular weight relative to the enzyme, passes freely through [5].…”

Section: Introductionmentioning

confidence: 99%

Modelling of immobilised enzyme biocatalytic membrane reactor performance

Preez

Clarke

Callanan

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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Immobilised enzyme-catalysed conversions frequently provide specific advantages of selectivity over chemical conversions and further, facilitate continuous operation through biocatalyst retention and reuse. This study focuses on the development and modelling of an enzyme-catalysed continuous immobilised enzyme biocatalytic membrane reactor (BMR).The conversion of the amidase-catalysed lactamide to lactic acid process was used as an industrially representative system with which to evaluate the process performance of the BMR.The model was developed from unsteady state differential mass balances incorporating a second order enzyme decay. This model was validated from empirically determined conversions in dual experiments using 80 and 40 mM amide substrate, 6.4 and 20.1 mg immobilised amidase and a flow rate of 0.0005 and 0.0001 L/min respectively.Model predictions over a range of amidase amounts and stabilities, flow rates and initial amide concentrations quantified the direction and extent of the influence of these parameters on the maximum conversions attainable, consequently identifying the critical parameter ranges defining optimal BMR performance. Although the model has been developed and validated for the prediction of BMR performance of the specific lactamide-lactic acid system,

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Developments in nitrile and amide biotransformation processes

Cited by 52 publications

References 88 publications

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Highly efficient synthesis of 5‐cyanovaleramide by Rhodococcus ruber CGMCC3090 resting cells

Modelling of immobilised enzyme biocatalytic membrane reactor performance

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