Dexamethasone-induced Changes in Sympathetic Innervation of Porcine Ovaries and in Their Steroidogenic Activity

Jana, Barbara; Dzienis, Anna; Rogozinska, Anna; Piskuła, Mariusz K.; Jedlinska-Krakowska, M; Wojtkiewicz, Joanna; Majewski, Mariusz

doi:10.1262/jrd.17019

Cited by 16 publications

(13 citation statements)

References 30 publications

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“…Glucocorticoids have been shown to enhance sympathetic innervation of other tissues (e.g., ovary) (Jana et al, 2005), and they can interact with ␤-adrenergic signaling to enhance the transcription of NGF (Colangelo et al, 2004). However, previous studies have shown that the social stress model used here leads to a reduction in circulating glucocorticoid levels ), which would not be able to explain the increased innervation observed in the current study.…”

Section: Discussioncontrasting

confidence: 73%

Social Stress Enhances Sympathetic Innervation of Primate Lymph Nodes: Mechanisms and Implications for Viral Pathogenesis

Sloan¹,

et al. 2007

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Behavioral processes regulate immune system function in part via direct sympathetic innervation of lymphoid organs, but little is known about the factors that regulate the architecture of neural fibers in lymphoid tissues. In the present study, we find that experimentally imposed social stress can enhance the density of catecholaminergic neural fibers within axillary lymph nodes from adult rhesus macaques. This effect is linked to increased transcription of the key sympathetic neurotrophin nerve growth factor and occurs predominately in extrafollicular regions of the paracortex that contain T-lymphocytes and macrophages. Functional consequences of stressinduced increases in innervation density include reduced type I interferon response to viral infection and increased replication of the simian immunodeficiency virus. These data reveal a surprising degree of behaviorally induced plasticity in the structure of lymphoid innervation and define a novel pathway by which social factors can modulate immune response and viral pathogenesis.

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Section: Discussioncontrasting

confidence: 73%

Social Stress Enhances Sympathetic Innervation of Primate Lymph Nodes: Mechanisms and Implications for Viral Pathogenesis

Sloan¹,

et al. 2007

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“…The weight, length and height of the pathologically changed ovaries were diminished, what may results from the presence of partially luteinized follicular cysts, the reduction in the number of small ovarian follicles (1-3 mm in diameter) and a total lack of both the medium-sized follicles and CL. Similar results were previously found in the pigs after adrenocorticotropic hormone (ACTH) or DXM treatment [9,11,20,21].…”

Section: Discussionsupporting

confidence: 88%

“…Furthermore, our pilot study showed that dexamethasone (DXM) injections in gilts from day 16 of the first studied estrous cycle until day 9 of the second studied cycle, lead to changes in the of P450 scc , 3β-HSD and P450 arom protein expression, as well as in the content of progesterone (P 4 ), androgens and estrogens in ovarian structures [9]. It is generally known that the morphological and functional changes of polycystic ovaries are closely dependent on the phase of estrous cycle, in which the administration of cyst-induced hormones was started [10][11][12].…”

Section: Introductionmentioning

confidence: 96%

“…It is generally known that the morphological and functional changes of polycystic ovaries are closely dependent on the phase of estrous cycle, in which the administration of cyst-induced hormones was started [10][11][12]. Thus, it may be hypothesized that the beginning of DXM treatment in the luteal phase of the estrous cycle may induce the changes in the ovarian morphology and steroidogenic activity, different from those found after induction of cysts formation during the follicular phase of the estrous cycle [9].…”

Section: Introductionmentioning

confidence: 99%

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Expression of steroidogenic enzymes in porcine polycystic ovaries.

Kozłowska

Majewski²,

Jana³

2009

Folia Histochem Cytobiol.

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Abstract:In the present study the expression pattern of the cholesterol side-chain cleavage cytochrome (P450 scc ), 3β-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (P450 arom ) was analyzed in the health and polycystic ovaries of gilts by means of the Western blot and immunohistochemistry. The polycystic status of ovaries was induced by i.m. dexamethasone (DXM) injections on days 7-21 of the estrous cycle. Macroscopic observation of ovaries of DXM-treated gilts revealed the presence of cysts (1-2 cm in diameter, with a mean number of 7.0±1.2 per ovary), a decrease (P<0.05) in number of small follicles (1-3 mm in diameter), as well as the lack of medium-sized follicles (4-6 mm in diameter) and corpora lutea, as compared to the control animals. The expression of P450 scc (P<0.01), 3β-HSD (P<0.05) and P450 arom (P<0.001) proteins in the cysts was higher than in the medium-sized follicles of the control gilts. Moreover, DXM injections resulted also in an enhancement (P<0.05) in the level of P450 scc protein in the walls of small follicles as compared to the control gilts. Following DXM administration the immunoreactivity (IR) of P450 scc in the primordial follicles was lower than in the control group. Comparing to the control gilts, the reaction for this enzyme in DXM-treated animals was observed in secondary follicles, while for 3β-HSD, in primordial, primary, as well as secondary follicles. The immunostaining for P450 scc (theca cells) and P450 arom (granulosa cells) in the small follicles of the DXM-treated gilts were more prominent than those found in the gonads of control animals. However, IR for P450 scc was not found in the granulosa cells of small follicles in the gilts receiving DXM. The intensity of P450 scc and P450 arom labelling was distinctly enhanced in the cysts as compared to the medium follicles of the control animals. Furthermore, in contrary to the medium follicles of the control animals, faint IR for 3β -HSD was found in the granulosa cell layer of cysts. Our data revealed that both the expression of P450 scc , 3β-HSD and P450 arom and localization of these enzymes in polycystic ovaries were different from those, found under physiological conditions. These results suggested that above-mentioned enzymes may, by influencing the ovarian steroid synthesis, play an essential role in the creation and/or course of cystic ovarian disease.

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“…It has recently been shown that not only is the density of sympathetic nerve fibers increased in the cystic ovaries of women [4], rats [5,6] and pigs [7], but also the cholinergic innervation undergoes a profound remodeling in porcine ovaries treated with dexamethasone phosphate disodium salt (DXM) [8]. Furthermore, it has been found that laparoscopic resection or cauterization of the medullar part of cystic ovaries -i.e.…”

Section: Introductionmentioning

confidence: 99%

Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

Kozłowska

Wojtkiewicz²,

Majewski³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:In a previous study, we showed that both the noradrenergic and cholinergic component of ovarian innervation is markedly changed in porcine cystic ovaries. The present study is aimed at elucidating the distribution pattern of substance P-(SP), calcitonin gene related peptide CGRP-and/or galanin (GAL)-containing nerve fibers within porcine cystic ovaries. The status polycysticus was induced by dexamethasone phosphate disodium salt i.m. injections performed from the 7 th until the 21 st day of the first studied estrous cycle. During the same period of time, gilts of the control group received saline. All animals were slaughtered on the expected 11 th day of the second studied estrous cycle, and their ovaries were collected. When compared to control gonad, a distinct difference in the distribution pattern and the density of SP-, CGRP-and/or GAL-immunoreactive (GAL-IR) nerve fibers was observed. Thus, unlike in the control gonad, SP-and/or CGRP-IR perivascular nerve fibers were found to supply medullar blood vessels of polycystic ovary. Furthermore, the number of GAL-IR nerve fibers contributing to the ground plexus in polycystic ovaries was higher than that observed in the control gonads. Thus, as may be judged from the profound changes in the distribution pattern of differently chemically coded afferent terminals within polycystic gonads, it appears possible that neuropeptides released from these terminals may take part in the etiopathogenesis of this disorder.

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Dexamethasone-induced Changes in Sympathetic Innervation of Porcine Ovaries and in Their Steroidogenic Activity

Cited by 16 publications

References 30 publications

Social Stress Enhances Sympathetic Innervation of Primate Lymph Nodes: Mechanisms and Implications for Viral Pathogenesis

Social Stress Enhances Sympathetic Innervation of Primate Lymph Nodes: Mechanisms and Implications for Viral Pathogenesis

Expression of steroidogenic enzymes in porcine polycystic ovaries.

Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

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