Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Liu, Zhen Qi; Zhao, Wei; Zhu, Xv Long; Huang, Guangsu; Xu, Feng; Yang, Jianhai; Osada, Yoshihito; Zrı́nyi, Miklós; Li, Jian Hui; Chen, Yong Mei

doi:10.1016/j.colsurfb.2015.02.005

Cited by 84 publications

(74 citation statements)

References 57 publications

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“…In contrast, F(P1/D) (tri-PCG micelle entrapping DPMP: solution (A) and F(PA) (tri-PCG-Acryl micelle solution: Solution (B) showed weak cytotoxicity at concentrations above 0.01 wt % and 0.1 wt %, respectively. Liu et al reported that oligo-thiols such as dithiothreitol (DTT) are cytotoxic towards NIH/3T3 fibroblasts and rat bone marrow mesenchymal stem cells (BMSCs) [24]. Klouda et al reported that polymers with multivalent acryloyl groups showed cytotoxicity [25].…”

Section: Resultsmentioning

confidence: 99%

Peptide Drug Release Behavior from Biodegradable Temperature-Responsive Injectable Hydrogels Exhibiting Irreversible Gelation

Takata¹,

Takai²,

Yoshizaki

et al. 2017

Gels

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Abstract:We investigated the release behavior of glucagon-like peptide-1 (GLP-1) from a biodegradable injectable polymer (IP) hydrogel. This hydrogel shows temperature-responsive irreversible gelation due to the covalent bond formation through a thiol-ene reaction. In vitro sustained release of GLP-1 from an irreversible IP formulation (F(P1/D+PA 40 )) was observed compared with a reversible (physical gelation) IP formulation (F(P1)). Moreover, pharmaceutically active levels of GLP-1 were maintained in blood after subcutaneous injection of the irreversible IP formulation into rats. This system should be useful for the minimally invasive sustained drug release of peptide drugs and other water-soluble bioactive reagents.

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Section: Resultsmentioning

confidence: 99%

Peptide Drug Release Behavior from Biodegradable Temperature-Responsive Injectable Hydrogels Exhibiting Irreversible Gelation

Takata¹,

Takai²,

Yoshizaki

et al. 2017

Gels

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“…Besides the polysaccharides discussed so far, which have widely been combined with gelatin, in this section, less‐explored polysaccharides for 3D cellular engineering and therapeutic applications will be highlighted. Table presents these polysaccharides with their structure and applications …”

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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“…Soft and biocompatible hydrogel, possessing three-dimensional (3D) polymer networks and physicochemical properties similar to the extracellular matrices of tissues891011, can provide the 3D microenvironment for the differentiation and engraftment of transplanted NSCs. Injectable hydrogel, delivering cells into localized lesion site within any defect shape via minimally invasive manner, has been extensively applied in the delivery of NSCs121314.…”

mentioning

confidence: 99%

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

Zhao¹,

Zhao²,

Chen³

et al. 2016

Sci Rep

Self Cite

137

103

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Self-healing injectable hydrogels can be formulated as three-dimensional carriers for the treatment of neurological diseases with desirable advantages, such as avoiding the potential risks of cell loss during injection, protecting cells from the shearing force of injection. However, the demands for biocompatible self-healing injectable hydrogels to meet above requirements and to promote the differentiation of neural stem cells (NSCs) into neurons remain a challenge. Herein, we developed a biocompatible self-healing polysaccharide-based hydrogel system as a novel injectable carrier for the delivery of NSCs. N-carboxyethyl chitosan (CEC) and oxidized sodium alginate (OSA) are the main backbones of the hydrogel networks, denoted as CEC-l-OSA hydrogel (“l” means “linked-by”). Owing to the dynamic imine cross-links formed by a Schiff reaction between amino groups on CEC and aldehyde groups on OSA, the hydrogel possesses the ability to self-heal into a integrity after being injected from needles under physiological conditions. The CEC-l-OSA hydrogel in which the stiffness mimicking nature brain tissues (100~1000 Pa) can be finely tuned to support the proliferation and neuronal differentiation of NSCs. The multi-functional, injectable, and self-healing CEC-l-OSA hydrogels hold great promises for NSC transplantation and further treatment of neurological diseases.

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Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Cited by 84 publications

References 57 publications

Peptide Drug Release Behavior from Biodegradable Temperature-Responsive Injectable Hydrogels Exhibiting Irreversible Gelation

Peptide Drug Release Behavior from Biodegradable Temperature-Responsive Injectable Hydrogels Exhibiting Irreversible Gelation

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

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