Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior

Komoto, Miki; Asada, Ayumi; Ohshima, Yasuyuki; Miyanaga, Kayo; Morimoto, Hirotoshi; Yasukawa, Takeshi; Morito, Katsuya; Takayama, Kentaro; Uozumi, Yoshinobu; Nagasawa, Kohei

doi:10.1016/j.lfs.2021.120217

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…According to our previous study, 13) to induce experimental colitis in C57BL/6J mice, we used DSS (dextran sulfate sodium salt, colitis grade, Cat. No.…”

Section: Induction Of Colitismentioning

confidence: 99%

“…Daily evaluation of the colitis severity was performed with disease activity index (DAI) scores, which are the sums of the scores for body weight loss (0 ≦ 5%, 1 = 5-10%, 2 = 11-15%, 3 = 16-20%), stool consistency (0 = normal, 1 = soft but still formed, 2 = very soft, 3 = diarrhea), and rectal bleeding (0 = no blood, 1 = occult blood in stool, 2 = trace of blood visible in stool, 3 = rectal bleeding), and scoring was performed blindly by at least two independent investigators. 9,13) In the following experiments, 10 mice in each group were divided equally (5 mice/group) into histological and biochemical examinations, and their DAI scores were almost the same between the two examinations (DAI scores on Day 11 were 0.6 ± 0.894 and 0.4 ± 0.548 in control group, 5 ± 0.707 and 3.6 ± 1.14 in DSS group, 0.6 ± 0.548 and 0.2 ± 0.447 in MgO group, and 3.6 ± 1.67 and 3.4 ± 1.52 in MgO + DSS group, respectively).…”

Section: Induction Of Colitismentioning

confidence: 99%

“…The expression levels of mRNAs were corrected as to that of β-actin and are given as % of the control. 13,16) 16S Ribosomal RNA (rRNA) Metagenome Analysis Total DNA was obtained from cecum feces, and the V3-V4 hypervariable region of 16S rRNA was amplified, followed by sequencing on an Illumina MiSeq platform (Illumina, Inc., CA, U.S.A.). Bioinformatic sequence analysis was carried out using a QIIME II pipeline (ver.…”

Section: Induction Of Colitismentioning

confidence: 99%

“…2021.2) with SILVA data base release 13_8. 13,17) Statistical Analysis Each experimental result is given as the mean ± standard deviation (S.D. ), and statistical significance was evaluated by two-way repeated measures ANOVA (Fig.…”

Section: Induction Of Colitismentioning

confidence: 99%

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Prophylactic Administration of Magnesium Oxide Prevents Dextran Sulfate Sodium-Induced Colonic Injury in Mice

Miyanaga

Asada

Komoto

et al. 2022

Biological & Pharmaceutical Bulletin

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We previously demonstrated that per os administration and ad libitum ingestion of a magnesium chloride (MgCl 2 ) solution had a prophylactic effect on dextran sulfate sodium (DSS)-induced colitis in mice, magnesium being considered to play a role in this preferable action. Magnesium oxide (MgO) is a commercially available magnesium formulation, but whether or not it prevents development of colitis is unknown. In this study, we investigated the effect of MgO administration on development of colitis in DSS-treated male C57BL/6J mice. Experimental colitis was induced by ad libitum ingestion of 1% (w/v) DSS, and the colitis severity was evaluated by disease activity index (DAI) scores, histological assessment and colonic expression of inflammatory cytokines. A 1 mg/mL MgO solution was administered to mice through ad libitum ingestion from a day before DSS treatment to the end of the experimental period of 12 d. In addition, the effects of DSS, MgO and their combination on the gut microbiota were investigated by 16S ribosomal RNA metagenome analysis. DSS-induced elevation of DAI scores was partially but significantly decreased by MgO administration, while MgO administration had no apparent effect on the shortened colonic length, elevated mRNA expression of colonic interleukin-1β and tumor necrosis factor-α, increased accumulation of colonic mast cells, or altered features of the gut microbiota in DSS-treated mice. Overall, we demonstrated that MgO had a prophylactic effect on the development of colitis in DSS-treated mice by preventing histological colonic damage, but not colonic inflammation or alteration of the gut microbiota.

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“…According to our previous study, 13) to induce experimental colitis in C57BL/6J mice, we used DSS (dextran sulfate sodium salt, colitis grade, Cat. No.…”

Section: Induction Of Colitismentioning

confidence: 99%

Section: Induction Of Colitismentioning

confidence: 99%

Section: Induction Of Colitismentioning

confidence: 99%

Section: Induction Of Colitismentioning

confidence: 99%

See 2 more Smart Citations

Prophylactic Administration of Magnesium Oxide Prevents Dextran Sulfate Sodium-Induced Colonic Injury in Mice

Miyanaga

Asada

Komoto

et al. 2022

Biological & Pharmaceutical Bulletin

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“…Mice models have been employed to understand the aftereffects of intestinal inflammation ( 6 ). For example, stress-associated behavior of chemically-induced colitis model ( 7 , 8 ) and compromised cognitive ability after the consumption of high-fat inflammatory diet ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Developmental defects and behavioral changes in a diet-induced inflammation model of zebrafish

Rehman

Gora²,

Varshney³

et al. 2022

Front. Immunol.

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Soybean meal evokes diet-induced intestinal inflammation in certain fishes. Although the molecular aspects of soybean-induced intestinal inflammation in zebrafish are known, the impact of the inflammatory diet on fish behavior remain largely underexplored. We fed zebrafish larvae with three diets - control, soybean meal and soybean meal with β-glucan to gain deeper insight into the behavioral changes associated with the soybean meal-induced inflammation model. We assessed the effect of the diets on the locomotor behavior, morphological development, oxygen consumption and larval transcriptome. Our study revealed that dietary soybean meal can reduce the locomotor activity, induce developmental defects and increase the oxygen demand in zebrafish larvae. Transcriptomic analysis pointed to the suppression of genes linked to visual perception, organ development, phototransduction pathway and activation of genes linked to the steroid biosynthesis pathway. On the contrary, β-glucan, an anti-inflammatory feed additive, counteracted the behavioral and phenotypic changes linked to dietary soybean. Although we did not identify any differentially expressed genes from the soybean meal alone fed group vs soybean meal + β-glucan-fed group comparison, the unique genes from the comparisons of the two groups with the control likely indicate reduction in inflammatory cytokine signaling, inhibition of proteolysis and induction of epigenetic modifications by the dietary glucan. Furthermore, we found that feeding an inflammatory diet at the larval stage can lead to long-lasting developmental defects. In conclusion, our study reveals the extra-intestinal manifestations associated with soybean meal-induced inflammation model.

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Sub-chronic and mild social defeat stress exposure to C57BL/6J mice increases visceral fat mass and causes accumulation of cholesterol and bile acids in the liver

Morito,

Yamagata,

Naka

et al. 2024

Biochemical and Biophysical Research Communications

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Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior

Cited by 8 publications

References 42 publications

Prophylactic Administration of Magnesium Oxide Prevents Dextran Sulfate Sodium-Induced Colonic Injury in Mice

Prophylactic Administration of Magnesium Oxide Prevents Dextran Sulfate Sodium-Induced Colonic Injury in Mice

Developmental defects and behavioral changes in a diet-induced inflammation model of zebrafish

Sub-chronic and mild social defeat stress exposure to C57BL/6J mice increases visceral fat mass and causes accumulation of cholesterol and bile acids in the liver

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