DFF45 expression in ovarian endometriomas

Banaś, Tomasz; Skotniczny, Krzysztof; Basta, Antoni

doi:10.1016/j.ejogrb.2009.05.024

Cited by 8 publications

(14 citation statements)

References 20 publications

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“…Since the cleavage of DFF45 by caspase-3 is an important step in the apoptotic pathway, down-regulation or loss of DFF45 expression in neoplastic tissues might result in the blockage of the caspase-3 mediated apoptotic pathway and consequently lead to tumor progression. Thus, our findings are in agreement with the tumor suppressor role of DFF45 [4,11,26].…”

Section: Discussionsupporting

confidence: 82%

“…DNA fragmentation factor 45 forms a heterodimer with DFF40, a 40 kDa endonuclease, and acts both as its specific inhibitor and as a chaperone in its appropriate folding [7,8]. When caspase-3 is activated by apoptotic stimuli, it cleaves DFF45 at two sites, which causes the release and activation of DFF40, leading to the generation of double-stranded breaks in internucleosomal chromatin regions and chromatin condensation [11]. Thus, aberrant DFF45 expression might influence tumor development and progression and DFF45 may play a role in malignant transformation and metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…Recent clinical studies have convincingly linked DFF45 with tumor progression and poor clinical outcomes in many cancer types, including neuroblastoma, esophageal carcinoma, ovarian endometriomas [9][10][11], and endometrial and ovarian carcinoma [10,[12][13][14][15]. However, the involvement of DFF45 in human GBC has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Clinicopathological significance of DNA fragmentation factor 45 and thyroid transcription factor 1 expression in benign and malignant lesions of the gallbladder

Yuan

Yang²,

Zou³

et al. 2013

pjp

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Gallbladder cancer (GBC) is one of the most aggressive tumors; we examined the expression level of DNA fragmentation factor 45 (DFF45) and thyroid transcription factor 1 (TTF-1) in benign and malignant lesions of the gallbladder by immunohistochemistry. The results were correlated with clinicopathological features and prognosis. DNA fragmentation factor 45 and TTF-1 expression was significantly higher in gallbladder adenocarcinomas than in the corresponding peritumoral tissues (χ 2 DFF45 = 6.92, χ 2 TTF-1 = 8.68, ps < 0.01), polyps (χ 2 DFF45 = 4.49, χ 2 TTF-1 = 5.35, ps < 0.05), and chronic cholecystitis (χ 2 DFF45 = 12.98, χ 2 TTF-1 = 17.74, ps < 0.01). Negative expression of DFF45 and TTF-1 was significantly associated with tumor differentiation, tumor mass, lymph node metastasis and invasion of adenocarcinomas (p < 0.05). Univariate Kaplan-Meier analysis showed that elevated expression levels of DFF45 and TTF-1 (p < 0.05) were closely associated with increased overall survival. In addition, the average survival time of patients with DFF45(+) TTF-1(+) tumors was significantly higher than those with DFF45(-) TTF-1(-) tumors (p < 0.05). Finally, multivariate Cox regression analysis showed that negative expression of DFF45 and TTF-1 was an independent prognostic predictor in gallbladder adenocarcinoma (p < 0.05). The expression of DFF45 and/or TTF-1 is closely related to the carcinogenesis, progression, clinical behavior and prognosis of gallbladder adenocarcinomas. DNA fragmentation factor 45 and TTF-1 could be progression-associated genes correlating with good prognosis in GBC.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Clinicopathological significance of DNA fragmentation factor 45 and thyroid transcription factor 1 expression in benign and malignant lesions of the gallbladder

Yuan

Yang²,

Zou³

et al. 2013

pjp

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“…To identify DFF45-positive cells and to assess the DFF45 level, the immunohistochemistry and Western blot technique were applied, respectively, as described in our previous studies [15,16,17]. DFF45-positive cells were counted independently by two histopathologists in 10 high-power fields of each slide.…”

Section: Methodsmentioning

confidence: 99%

“…DFF45 expression has been already investigated in several pathologies of the female genital tract including endometriosis, endometrial hyperplasia and ovarian cancer [14,15,16,17,18,19]. However, for a better understanding, it seems reasonable to determine DFF45 expression in normal human endometrium as well.…”

Section: Introductionmentioning

confidence: 99%

DFF45 Expression in Human Endometrium Is Associated with Menstrual Cycle Phases and Decreases after Menopause

Banaś

Basta²,

Knafel³

et al. 2012

Gynecol Obstet Invest

Self Cite

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Background: Human endometrium undergoes cyclic structural and functional modifications, and if no conception occurs menstruation is observed as the result of endometrial cell apoptosis via DFF40/DFF45 complex activation. In postmenopausal endometrium, the proliferative potential of endometrial cells is decreased, while their susceptibility to apoptosis increases. Methods: The study group comprised 104 nonpregnant adult women (78 of reproductive age and 36 after menopause) with no neoplasm or hormonal treatment during the past 6 months. Immunohistochemistry and Western blot methods were used for DFF45 identification and semiquantitative assessment of its amount. Results: Significantly more DFF45-positive cells were detected in the endometrial glands compared to stroma, and this pattern was constant throughout the whole menstrual cycle and also present in postmenopausal endometrial species. The lowest mean relative amount of DFF45 was detected in postmenopausal endometrial samples. In women of reproductive age, the highest mean relative amount of DFF45 was identified in an early secretory phase of the menstrual cycle, the lowest median value of the relative amount of DFF45 was observed in the late proliferative phase, and the difference was significant. Conclusion: The DFF45 level in human endometrium corresponds to the respective phase of the menstrual cycle and decreases significantly after menopause.

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Differential expression of DNA fragmentation factor 45 in leukoplakia and oral squamous cell carcinoma and its correlation with TNF-α levels

Balakrishnan,

Ramadoss,

Padmanaban

et al. 2024

Oral Oncology Reports

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DFF45 expression in ovarian endometriomas

Cited by 8 publications

References 20 publications

Clinicopathological significance of DNA fragmentation factor 45 and thyroid transcription factor 1 expression in benign and malignant lesions of the gallbladder

Clinicopathological significance of DNA fragmentation factor 45 and thyroid transcription factor 1 expression in benign and malignant lesions of the gallbladder

DFF45 Expression in Human Endometrium Is Associated with Menstrual Cycle Phases and Decreases after Menopause

Differential expression of DNA fragmentation factor 45 in leukoplakia and oral squamous cell carcinoma and its correlation with TNF-α levels

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