DIA (Data Independent Acquisition) proteomic based study on maize filling-kernel stage drought stress-responsive proteins and metabolic pathways

Yang, Yatong; Dong, Anyi; Zenda, Tinashe; Liu, Songtao; Li, Jiao; Duan, Huijun

doi:10.1080/13102818.2020.1827981

Cited by 2 publications

(2 citation statements)

References 95 publications

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“…The Orbitrap Lumos mass spectrometer was set to automatically switch between MS and MS/MS acquisition in data-dependent acquisition (DDA) mode for library generation, the parameters were as previously described [27]. The data-independent acquisition (DIA) mode for quantitative samples with 60 variable isolation windows overlapped at 1 m/z.…”

Section: Nano-hplc-ms/ms Analysismentioning

confidence: 99%

Proteomics Analysis and Identification of Proteins Related to Isoprenoid Biosynthesis in Cinnamomum camphora (L.) Presl

Chang-san¹,

Zhang

Chen

et al. 2022

Forests

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Cinnamomum camphora is an evergreen tree that contains essential oils and the leaf is the main organ used to extract essential oils. Isoprenoid is a key component contributing to the essential oils of C. camphora. Still, the molecular mechanisms and regulatory pathways underlying isoprenoid biosynthesis remain to be explored. In our study, we found that the content of linalool was higher in 65-day-old leaves than that in 15-day-old leaves of linalool-type C. camphora. The leaf proteome of these two materials was then analyzed based on data-independent acquisition, respectively, and 11,503 proteins were identified, of which 11,076 were annotated. Analysis of differentially expressed proteins revealed that the expression levels of MCT, MDS, and AACT increased significantly in 65-day-old leaves. Further analysis of the protein interaction network indicated that 15 differentially expressed proteins related to isoprenoid biosynthesis were co-expressed, and also suggested that the transcription factor families of BES1, C3H, MYB, NF-YC, Nin-like, WRKY, ZF-HD, and bHLH may act as candidate regulators of leaf development in C. camphora. Our study verified that the process of isoprenoid biosynthesis in C. camphora is regulated by a complicated network consisting of conserved synthetases, and provides proteomic information for further investigation of isoprenoid metabolic.

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Section: Nano-hplc-ms/ms Analysismentioning

confidence: 99%

Proteomics Analysis and Identification of Proteins Related to Isoprenoid Biosynthesis in Cinnamomum camphora (L.) Presl

Chang-san¹,

Zhang

Chen

et al. 2022

Forests

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“…Active also during mesocotyl growth, as well as in photosynthetic carbon metabolism reprogramming (particularly under high CO 2 conditions), they are also accumulated in ABA-deficient mutants. In some QTL studies, SmallHSPs have already been associated with physiological characteristics related to seed longevity (Arif et al 2022 ), senescence (Caicedo et al 2021 ), grain filling (Yang et al 2020 ) and other quantitative traits (Zhou et al 2021 a ; Zhou et al 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Maize heat shock proteins—prospection, validation, categorization and in silico analysis of the different ZmHSP families

Diogo-,

de Resende Von Pinho,

Pinto

et al. 2023

Stress Biology

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Among the plant molecular mechanisms capable of effectively mitigating the effects of adverse weather conditions, the heat shock proteins (HSPs), a group of chaperones with multiple functions, stand out. At a time of full progress on the omic sciences, they look very promising in the genetic engineering field, especially in order to conceive superior genotypes, potentially tolerant to abiotic stresses (AbSts). Recently, some works concerning certain families of maize HSPs (ZmHSPs) were published. However, there was still a lack of a study that, with a high degree of criteria, would fully conglomerate them. Using distinct but complementary strategies, we have prospected as many ZmHSPs candidates as possible, gathering more than a thousand accessions. After detailed data mining, we accounted for 182 validated ones, belonging to seven families, which were subcategorized into classes with potential for functional parity. In them, we identified dozens of motifs with some degree of similarity with proteins from different kingdoms, which may help explain some of their still poorly understood means of action. Through in silico and in vitro approaches, we compared their expression levels after controlled exposure to several AbSts' sources, applied at diverse tissues, on varied phenological stages. Based on gene ontology concepts, we still analyzed them from different perspectives of term enrichment. We have also searched, in model plants and close species, for potentially orthologous genes. With all these new insights, which culminated in a plentiful supplementary material, rich in tables, we aim to constitute a fertile consultation source for those maize researchers attracted by these interesting stress proteins.

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DIA (Data Independent Acquisition) proteomic based study on maize filling-kernel stage drought stress-responsive proteins and metabolic pathways

Abstract: Duan (2020) DIA (Data Independent Acquisition) proteomic based study on maize filling-kernel stage drought stress-responsive proteins and metabolic pathways,

Cited by 2 publications

References 95 publications

Proteomics Analysis and Identification of Proteins Related to Isoprenoid Biosynthesis in Cinnamomum camphora (L.) Presl

Proteomics Analysis and Identification of Proteins Related to Isoprenoid Biosynthesis in Cinnamomum camphora (L.) Presl

Maize heat shock proteins—prospection, validation, categorization and in silico analysis of the different ZmHSP families

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