Diabetes mellitus: Polymorphonuclear leukocyte (PMN) filtration parameters and PMN membrane fluidity after chemotactic activation

LoPresti, Rosalia; Montana, M.; Canino, Baldassare; Ventimiglia, G.; Catania, Anna; Caimi, Gregorio

doi:10.1016/s0026-0495(99)90006-9

Cited by 5 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant association has been reported between WBC count and insulin resistance in subjects without diabetes. 9 Filtration parameters and membrane fluidity of polymorphonuclear leukocytes are reported to differ between normal subjects and patients with diabetes, 38 however, in the present study, fasting serum insulin levels and insulin resistance were not found to be associated with WBC count. This discrepancy may be explained by differences in study populations, as subjects in the present study were relatively young, with a mean age of 24.1 years, and the mean age of subjects in one of the published studies was 49.5 years.…”

Section: Discussioncontrasting

confidence: 78%

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

et al. 2016

View full text Add to dashboard Cite

ObjectivesTo evaluate fasting serum insulin levels and insulin resistance, and their association with blood rheology, in Japanese young adults without diabetes.MethodsBlood samples were analysed and blood rheology was estimated using haematological parameters. Whole blood passage time was measured using a Hitachi MC-FAN© microchannel array flow analyser.ResultsOut of 151 subjects (mean age, 24.1 ± 1.5 years), fasting serum insulin levels and insulin resistance (using homeostasis model assessment-estimated insulin resistance [HOMA-IR]), were positively correlated with longer whole blood passage times and higher values for haematocrit (Hct), haemoglobin (Hb), fibrinogen, body weight, body mass index (BMI), triglycerides, and low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein cholesterol (HDL-C) ratio, and were negatively correlated with HDL-C. Whole blood passage time correlated with body weight, BMI, LDL-C/HDL-C ratio, Hct, Hb, white blood cell (WBC) count, platelet count, fibrinogen, fasting serum insulin levels, and HOMA-IR. Multiple regression analysis revealed that whole blood passage time was independently associated with Hct, fibrinogen levels, and WBC count.ConclusionsFasting serum insulin levels and insulin resistance were associated with blood rheology, and may influence blood rheology by modulating haematological parameters and lipid parameters in young adults without diabetes.

show abstract

Section: Discussioncontrasting

confidence: 78%

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The principal parameters measured in this group were: blood urea nitrogen (40.3 ± 29.4 mmol/L, 112.9 ± 82.3 mg/dL), serum creatinine (343.9 ± 246.6 μmol/L, 3.9 ± 2.8 mg/dL; range 159.1–680.7 μmol/L, 1.8–7.7 mg/dL), potassium (4.6 ± 0.6 mequiv./l) sodium (140.7 ± 5.9 mequiv./l), calcium (2.3 ± 0.2 mmol/L, 9.2 ± 0.8 mg/dL) and phosphate (1.36 ± 0.35 mmol/L, 4.2 ± 1.1 mg/dL). Diabetic and hypertensive patients were excluded from this group because these patients, as described previously, 20,21 exhibited altered PMN rheology and metabolism. No patient was treated with calcium channel blockers or active vitamin D metabolites.…”

Section: Methodsmentioning

confidence: 99%

Polymorphonuclear leucocyte rheology and cytosolic Ca²⁺ content after activation in chronic renal failure

et al. 2001

View full text Add to dashboard Cite

SUMMARY: We evaluated polymorphonuclear leucocyte (PMN) flow properties in patients with clinically stable chronic renal failure (CRF) and in control subjects at baseline and after activation with 4‐phorbol 12‐myristate 13‐acetate (PMA) and N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP). Initial relative flow rate (IRFR) and clogging particles (CPs) were obtained using the St. George's Filtrometer, and PMN membrane fluidity was assessed by marking PMNs with 1‐(4‐(trimethylamino)phenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH). PMN cytosolic Ca2+ concentration was determined by marking PMNs with Fura 2‐AM. At baseline, CRF patients showed a significant increase only in PMN cytosolic Ca2+ content. After activation with PMA and fMLP, a decrease in IRFR and an increase in CP were observed in both control subjects and CRF patients, although the variation in IRFR present in the group of CRF patients was greater than in the control group. After activation with PMA and fMLP, we found a decrease in PMN membrane fluidity only in CRF patients, but no variation in PMN cytosolic Ca2+ concentration in either group was observed. These results provide evidence for PMN dysfunction in chronic renal failure.

show abstract

“…Ca i mi et al 24 re por ted that PNL flu i dity was dec re a sed in pati ents with di a be tes mel li tus and the dec re a se was mo re pro no un ced in pa ti ents with type 2 di a be tes. In another study con duc ted by LoP res ti et al 25 it was fo und that PNL flu i dity was dif fe rent bet we en pa ti ents with di a be tes mel li tus and con trols.…”

Section: Fi Gu Rementioning

confidence: 87%

Alterations in Lymphocyte Membrane Protein Content and Increased Lymphocyte Rigidity in Cats with Diabetes Mellitus

Çefle¹,

Tamer²,

Albeniz³

et al. 2011

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : In a pre vi o us study we sho wed that red cell de for ma bi lity was dec re a sed in cats with nonin su lin de pen dent di a be tes mel li tus (NIDDM) and it was as so ci a ted with ab nor ma li ti es in va ri o us rhe o lo gi cal para me ters (blo od and plas ma vis co sity, eryt hrocy te de for ma bi lity, h e mo to lo gi cal pa ra me ters) as well as al te ra ti ons in red cell mem bra ne pro te ins. On the ot her hand, the re is li mi ted da ta con cer ning the con tri bu ti on of lymphocy te mem bra ne pro te in ab nor ma li ti es and in cre a sed lymphocy te ri gi dity to im pa i red blo od rhe o logy in di a be tes. In the pre sent study, we ai med to in ves ti ga te lymphocy te de for ma bi lity and al te ra ti ons in lymphocy te mem brane pro te ins in cats with NIDDM. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : In this re gard, we analy zed lymphocy te de for ma bi lity in 10 cats with non-in su lin de pen dent di a be tes mel li tus (ma le/fe ma le: 5/5, me an age: 3.08 ± 1.39 ye ars, me an weight: 3.26 ± 1.25 kg.) and 10 he althy con trols (ma le/fe ma le: 6/4, me an age 5.7 ± 1.91 ye ars, me an we ight: 4.56 ± 1.16 kg.) by using the mic ro fil ter tech ni qu e. We al so as ses sed mem bra ne pro te in con tent by so di um do decyl sulfa te-pol yacr yla mi de gel elec trop ho re sis (SDS-PA GE). In ad di ti on, blo od le vels of glu co se, to tal cho les te rol, highden sity li pop ro te in (HDL), low-den sity li pop ro te in (LDL), very low-den sity li pop ro te in (VLDL) and trygli ce ri de we re de ter mi ned. R Re e s su ul lt ts s: : We fo und that lymphocy te ri gi dity () was sig ni fi cantly in cre a sed in cats with NIDDM com pa red to con trols (1.215 ± 0.532 vs. 0.265 ± 0.42; p<0.01). Blo od (3.8 ± 0.1 vs 4.4 ± 0.2; p< 0.001) and plas ma vis co sity (1.2 ± 0.1 vs. 1.4 ± 0.1; p< 0.01) we re sig ni fi cantly hig her in the di a be tic gro up. Blo od le vels of glu co se (99.8 ± 21 mg/dL vs. 177.9 ± 53.2 mg/dL; p< 0.001), to tal cho les te rol (106.4 ± 15.6 mg/dL vs. 183.4 ± 57.8 mg/dL; p< 0.001), LDL (12.1 ± 5.3 mg/dL vs. 24.7 ± 14.6 mg/dL; p< 0.02) and HDL (78.7 ± 9.5 mg/dL vs. 133.4 ± 44.9 mg/dL; p< 0.001) we re sig ni fi cantly hig her in the di a be tic gro up. SDS-PA GE re ve a led that the band which corres ponds to the pro te in with a we ight of 37 kDA had di sap pe a red in cats with NIDDM. C Co on nc c l lu u s si i o on n: : We sug gest that the ob ser ved ab nor ma li ti es in mem bra ne pro te ins may play a ro le in re du ced lymphocy te de for ma bi lity asso ci a ted with di a be tes mel li tus and may ha ve a ro le in in cre a sed blo od vis co sity. K Ke ey y W Wo or rd ds s: : Di a be tes mel li tus; lymphocy tes; he mor he o logy; elec trop ho re sis, pol yacr yla mi de gel Ö ÖZ ZE ET T A Am ma aç ç: : Da ha ön ce ki bir ça lış ma da, in sü lin den ba ğım sız di a be tes mel li tus lu (NIDDM) ke di ler de erit ro sit şekil de ğiş ti re bi lir li ğin de ki azal ma nın mem bran pro te in le ri...

show abstract

Diabetes mellitus: Polymorphonuclear leukocyte (PMN) filtration parameters and PMN membrane fluidity after chemotactic activation

Cited by 5 publications

References 26 publications

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

Polymorphonuclear leucocyte rheology and cytosolic Ca²⁺ content after activation in chronic renal failure

Alterations in Lymphocyte Membrane Protein Content and Increased Lymphocyte Rigidity in Cats with Diabetes Mellitus

Contact Info

Product

Resources

About

Diabetes mellitus: Polymorphonuclear leukocyte (PMN) filtration parameters and PMN membrane fluidity after chemotactic activation

Cited by 5 publications

References 26 publications

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes

Polymorphonuclear leucocyte rheology and cytosolic Ca2+ content after activation in chronic renal failure

Alterations in Lymphocyte Membrane Protein Content and Increased Lymphocyte Rigidity in Cats with Diabetes Mellitus

Contact Info

Product

Resources

About

Polymorphonuclear leucocyte rheology and cytosolic Ca²⁺ content after activation in chronic renal failure