Diagnosis of Nasopharyngeal Carcinoma by DNA Amplification of Tissue Obtained by Fine-Needle Aspiration

Abstract

In patients with suspected nasopharyngeal carcinoma, fine-needle aspiration can provide tissue for diagnosis by DNA amplification of EBV genomes. The presence of EBV in metastases from an occult primary tumor is predictive of the development of overt nasopharyngeal carcinoma.

Genomic alterations in nasopharyngeal carcinoma: loss of heterozygosity and Epstein-Barr virus infection

“…DNA was extracted from the tumour tissues and blood leucocytes by methods previously described (Maniatis et al, 1989). EBV detection and typing by PCR For the detection and typing of EBV DNA in the tumour tissues, three previously described polymerase chain reaction (PCR) protocols were used with some modifications (Sample et al, 1990;Feinmesser et al, 1992;Lin et al, 1993). DNA from cell line B958, EBV-transformed human lymphocytes (American TIype Culture Collection), was used as positive control and double-distilled water as negative control.…”

“…The second amplified a human ,-actin genomic sequence, generating an approximately 318-bp DNA fragment. The oligonucleotide sequences for both sets of PCR primers were identical to the ones previously reported (Feinmesser et al, 1992).…”

Imaging in nasopharyngeal carcinoma: the value of 18-Florine Fluorodeoxyglucose PET/CT in comparison to conventional imaging modalities CT and MRI

“…The fusion of both imaging modalities in an integrated PET/CT machine should give better results. 14,[23][24][25] Furthermore, useful information can be obtained during a single seating and also time saving for the patient's convenience.…”

“…A fused integrated morphological and functional imaging modality of positron emission tomography / computed tomography (PET/CT) is another possible useful new tool to be utilized in the assessment of NPC patients like in other oncological patients. 14 This study was conducted in view to evaluate the role of fused PET/CT in the management of NPC patients.…”

Sustained Expression of the Novel EBV-Induced Zinc Finger Gene, ZNF EB, Is Critical for the Transition of B Lymphocyte Activation to Oncogenic Growth Transformation

“…RT-PCR products were electrophoretically separated. Where indicated, gels were blotted to Hybond-N ϩ membranes (Amersham Pharmacia Biotech) and hybridized with the following ␥-32 Pend-labeled reporter probes (23,24): ZNF EB and ZNF EB sv (ZNF EB 1858, 5Ј-CCATTGTGGAGAAGACAGTC-3Ј); EBNA1 (109194, 5Ј-TGAAT ACCACCAAGAAGGTG-3Ј); and ␤-glucuronidase (GUS 1405, 5Ј-CTACTACTTGAAGATGGTGATCG-3Ј). Photography of gels/blots was performed using a Chemi Imager (Alpha Innotech, San Leandro, CA).…”