Diagnostic application of KRAS mutation testing in uterine microglandular proliferations

Hong, Wei; Abi‐Raad, Rita; Alomari, Ahmed K.; Hui, Pei; Buza, Natália

doi:10.1016/j.humpath.2015.03.010

Cited by 11 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…various human cancers. Activated K-Ras signaling contributed to promoting tumor initiation and cell proliferation (17,18). Previous studies supported the idea that simultaneous activation of phosphatidylinositol 3-kinase (PI3K) was essential for K-Ras-induced tumorigenesis (19)(20)(21).…”

Section: Aspirin Inhibits the Proliferation Of Human Uterine Leiomyommentioning

confidence: 85%

Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K-Ras-p110α interaction

et al. 2017

View full text Add to dashboard Cite

Aspirin has been confirmed as an effective antitumor drug in various cancers. However, the relationship between aspirin and uterine leiomyoma is still underexplored. Here, we explored the effects of aspirin on human uterine leiomyoma cells and provide insights into the underlying mechanisms. Cell Counting Kit-8 (CCK-8) and flow cytometry analysis showed that aspirin treatment inhibited cell proliferation and promoted cell cycle arrest at G0/G1 phase in a dose- and time‑dependent manner of human uterine leiomyoma cells. Further studies revealed that aspirin blocked the interaction between K-Ras and p110α by co-immunoprecipitation and immunofluorescence. Western blotting demonstrated K‑Ras‑p110α interaction was required for the effects of aspirin‑induced inhibition on cell growth and cell cycle transition via cell cycle regulators, including cyclin D1 and cyclin-dependent kinase 2 (CDK2). PI3K/Akt/caspase signaling pathway was involved in human uterine leiomyoma cell growth under aspirin treatment. Taken together, these results suggest that aspirin inhibited human uterine leiomyoma cell growth by regulating K‑Ras‑p110α interaction. Aspirin which targeting on interaction between K-Ras and p110α may serve as a new therapeutic drug for uterine leiomyoma treatment.

show abstract

Section: Aspirin Inhibits the Proliferation Of Human Uterine Leiomyommentioning

confidence: 85%

Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K-Ras-p110α interaction

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Nonetheless, factors that have been found to favor endometrial carcinoma or AMGP over MGH include (1) postmenopausal age group125; (2) morphologic continuity between the microglandular areas and normal endometrium; (3) solid, nonsquamous morphology; (4) stromal cells with weak to moderate positivity for CD34 and strong positivity for CD1063; (5) diffuse p16 positivity63,131; (6) loss of expression of PAX2131; (7) presence of a KRAS mutation134; (8) foam cells and luminal squamous metaplasia64; (9) loss of expression of PTEN; (10) clinical/imaging features that suggest the presence of an endometrial mass or lesion. Factors that favor MGH over endometrial carcinoma or AMGP include (1) premenopausal age group; (2) stromal cells with weak to moderate positivity for CD10 and strong positivity for CD3463; (3) minimal or no expression of p1663,131; (4) retained expression of PAX2131; (5) absence of a KRAS mutation135; (6) subnuclear vacuoles and exclusively abluminal squamous metaplasia64; (7) morphologic continuity between microglandular areas and normal endocervical epithelium.…”

Section: Mucinous Carcinomamentioning

confidence: 99%

Mucinous Proliferations of the Uterine Corpus: Comprehensive Appraisal of an Evolving Spectrum of Neoplasms

Fadare¹

2022

Advances in Anatomic Pathology

View full text Add to dashboard Cite

A variety of endometrial lesions may contain mucinous cells. Herein, the author reviews the literature on the classification and clinicopathologic significance of uterine corpus proliferations with a significant mucinous component, assesses the 2020 World Health Organization classification of such lesions, and presents a diagnostic framework. The key epithelial mucinous lesions include mucinous metaplasia, atypical mucinous glandular proliferation and mucinous carcinoma. Each of these categories are classifiable into "usual" and gastrointestinal subtypes, the latter being indicative of intestinal (presence of goblet cells) and/or gastric-type (abundant, pale eosinophilic or clear cytoplasm and well-defined cell borders) morphology. It has been proposed that at least focal expression of gastrointestinal immunohistochemical markers be required for all gastrointestinal type lesions, and for gastrointestinal type atypical mucinous glandular proliferation and carcinoma, minimality or absence of estrogen receptor expression, and the absence of an endometrioid component. Mucinous carcinomas of the usual type, in which > 50% of the tumor is comprised of a mucinous component, are the most common. Morphologic subtypes include mucinous carcinoma with microglandular features and mucinous carcinoma with signet rings (signet ring carcinoma). Endometrioid carcinomas with a less than a 50% mucinous component are classified as endometrioid carcinoma with mucinous differentiation. Several studies have directly compared endometrioid and mucinous carcinomas, the latter presumably of the usual type, with respect to patient outcomes after treatment. All have found no difference in overall and disease free survival between these groups. However, three major studies have found mucinous carcinomas to be associated with a higher risk of lymph node metastases. Nineteen cases of mucinous carcinoma of the gastrointestinal type have been reported, and the limited data on their follow-up after primary treatment suggests that this subtype is more clinically aggressive and should accordingly be classified separately from mucinous carcinomas of the usual type. The morphologic spectrum of mucinous carcinoma of the gastrointestinal type is unclear and continues to evolve. Mucinous change, which may sometimes be extensive, may also be associated with papillary proliferation of the endometrium, adenomyoma of the endocervical type, atypical, and typical adenomyomas. In a curettage or biopsy, intestinal type mucinous epithelium may be indicative of any of the gastrointestinal lesions mentioned above, but may also represent samplings of uterine teratomas, yolk sac tumors, genital and extragenital adenocarcinomas with intestinal differentiation, or lowgrade appendiceal mucinous neoplasms that secondarily involve the endometrium.

show abstract

“…Prostate Cancer [107] Perianal Paget's Disease [108] Myxoid Liposarcoma [109] Malignant Fibrous Histiocytoma [110] Hemangiopericytoma [111] Cauda Equina Syndrome [112] Carcinoma of the Cervix [113] Carcinoma of the Vagina [114] Epidermoid Cysts [115] Prader-Willi Syndrome [116] Colorectal Carcinoma [117] Benign Prostate Hyperplasia [118] Microglandular Hyperplasia [119] Adenocarcinoma of Cervix [120] Adenoma Malignum [121] Adenosquamous Carcinoma of Cervix [122] Glassy Cell Carcinoma of Cervix [123] Adenoid Basal Carcinoma of Cervix [124] Adenomyosis [125] Uterine Fibroids [126] Endometrial Polyps [127] Hematoma [128] Fournier's Gangrene [129] Malakoplakia [130] Aneurysmal bone cyst [131] Ewing Sarcoma [132] Osteosarcoma [133] Chondrosarcoma [134] Neurofibroma [135] Neuroblastoma [136] Schwannoma [137] Chordoma [138] Malignant Schwannoma [139] Hemangioma [140] Myelolipoma [141] Leiomyoma [142] Villous Adenoma [143] Solitary Fibrous tumor [144] Castleman disease [145] Lymphoma [146] Gastrointestinal Stromal Tumor [147] Epithielial Malignancies …”

Section: Genetic Origins Of Uro-rectal Diseasesmentioning

confidence: 99%

Current Concepts and Evaluation of Uro-Rectal Diseases by Transrectal Ultrasound: A Review

Gautam¹,

Kelly²,

Reese³

2015

Adv Genet Eng

View full text Add to dashboard Cite

This article specifically discusses the clinical importance and current role of transrectal ultrasound (TRUS) to index the clinical suspicion of uro-rectal diseases. The traditional digital rectal examination is an inadequate method with low sensitivity and specificity, and confidence level for uro-rectal diseases. TRUS provides further imaging modalities, computerized tomography, magnetic resonance imaging, and three-dimensional reconstruction. Prostate cancer can be diagnosed in patient with refractory prostate-specific antigen to increase to increase diagnostic modalities with fusion biospies. The outcome of uro-rectal diseases can be improved by utilization of initial TRUS, a non-invasive and radiation free, extension of physical diagnosis.

show abstract

Diagnostic application of KRAS mutation testing in uterine microglandular proliferations

Cited by 11 publications

References 27 publications

Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K-Ras-p110α interaction

Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K-Ras-p110α interaction

Mucinous Proliferations of the Uterine Corpus: Comprehensive Appraisal of an Evolving Spectrum of Neoplasms

Current Concepts and Evaluation of Uro-Rectal Diseases by Transrectal Ultrasound: A Review

Contact Info

Product

Resources

About