Diagnostic Challenges for the Detection of Emerging Pathogens: A Case Study Involving the Incursion of Pseudomonas syringae pv. actinidiae in New Zealand

Taylor, Robert K.; Chapman, J. R.; Romberg, Megan K.; Weir, Bevan S.; Vanneste, J. L.; Everett, K.R.; Ward, L. I.; Liefting, L. W.; Lebas, B. S. M.; Alexander, B. J. R.

doi:10.1007/978-94-017-9020-8_5

Cited by 2 publications

(2 citation statements)

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“…To address this question, all Pseudomonas cultures in the ICMP isolated from kiwifruit were screened with the diagnostic primers, cultures that dated back to the 1970s. None of these isolates tested positive for Psa, suggesting the pathogen was not historically present in New Zealand [22]. However, the deposits of Pseudomonas spp.…”

Section: The Role Of Collections In Monitoring Plant Pathogenic Bacteriamentioning

confidence: 96%

“…This revealed four global populations [23] (Table 1). Multigene sequencing of Psa isolates revealed that there were two variants in New Zealand: the outbreak-virulent strain 'Psa-V' (=Psa3) and a less virulent strain 'Psa-LV' (=Psa4) [22]. With Psa-LV, the symptoms did not progress beyond leaf spotting.…”

Section: The Role Of Collections In Monitoring Plant Pathogenic Bacteriamentioning

confidence: 99%

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Building More Resilient Culture Collections: A Call for Increased Deposits of Plant-Associated Bacteria

et al. 2022

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Biological collections preserve our past, while helping protect our future and increase future knowledge. Plant bacterial culture collections are our security for domestic and global biosecurity. This feature article will provide an introduction to the global position of plant bacterial collections. The role of collections in monitoring plant pathogenic bacteria will be explored through the presentation of five cases studies. These case studies demonstrate why culture collections were imperative for the outcome in each situation. We discuss what we believe should be the best practices to improve microbial preservation and accessioning rates, and why plant bacterial culture collections must increase deposits to be prepared for future emerging pathogens. This is not only the case for global culture collections, but on a much bigger scale, our future scientific successes, our biosecurity decisions and responses, and our knowledge are contingent upon preserving our valuable bacterial strains. It is hoped that once you read this article, you will see the need to deposit your strains in registered public collections and make a concerted effort to build better bacterial culture collections with us.

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Section: The Role Of Collections In Monitoring Plant Pathogenic Bacteriamentioning

confidence: 96%

Section: The Role Of Collections In Monitoring Plant Pathogenic Bacteriamentioning

confidence: 99%

Building More Resilient Culture Collections: A Call for Increased Deposits of Plant-Associated Bacteria

et al. 2022

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Real-Time PCR Assays for the Detection of Puccinia psidii

Baskarathevan

Taylor

et al. 2016

Plant Disease

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Puccinia psidii (Myrtle rust) is an emerging pathogen that has a wide host range in the Myrtaceae family; it continues to show an increase in geographic range and is considered to be a significant threat to Myrtaceae plants worldwide. In this study, we describe the development and validation of three novel real-time polymerase reaction (qPCR) assays using ribosomal DNA and β-tubulin gene sequences to detect P. psidii. All qPCR assays were able to detect P. psidii DNA extracted from urediniospores and from infected plants, including asymptomatic leaf tissues. Depending on the gene target, qPCR was able to detect down to 0.011 pg of P. psidii DNA. The most optimum qPCR assay was shown to be highly specific, repeatable, and reproducible following testing using different qPCR reagents and real-time PCR platforms in different laboratories. In addition, a duplex qPCR assay was developed to allow coamplification of the cytochrome oxidase gene from host plants for use as an internal PCR control. The most optimum qPCR assay proved to be faster and more sensitive than the previously published nested PCR assay and will be particularly useful for high-throughput testing and to detect P. psidii at the early stages of infection, before the development of sporulating rust pustules.

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Diagnostic Challenges for the Detection of Emerging Pathogens: A Case Study Involving the Incursion of Pseudomonas syringae pv. actinidiae in New Zealand

Cited by 2 publications

References 28 publications

Building More Resilient Culture Collections: A Call for Increased Deposits of Plant-Associated Bacteria

Building More Resilient Culture Collections: A Call for Increased Deposits of Plant-Associated Bacteria

Real-Time PCR Assays for the Detection of Puccinia psidii

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