Diagnostic Devices for Isothermal Nucleic Acid Amplification

Chang, Chin‐Chen; Chen, Chien‐Cheng; Wei, Shih-Chung; Lu, Hsin-Min; Liang, Yu; Lin, Chii-Wann

doi:10.3390/s120608319

Cited by 131 publications

(68 citation statements)

References 99 publications

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“…C. Guatelli et al described the way in vitro amplifying nucleic acids isothermally depent on T7 RNA polymerase, RNase H and AMV reverse transcriptase, which was called 3SR in 1990. They also detected HIV-1 and preliminarily confirmed 3SR could be used to quantify and qualify special nucleic acid [1] . In 1991, Canadian scholar J. Compton named the assay with nucleic acid sequence-based amplification (NASBA).…”

Section: Development Of Nasbamentioning

confidence: 98%

“…The most widely used NASBA kit at present is NucliSens EasyQ assay which could detect Listeria, vibrio cholera, salmonella and so on [11] . At the same time, NABSA combined with a variety of detection technology to diagnose pathogens, including agarose gel electrophoresis [3] , an enzyme-linked gel assay (ELGA) [5] , electro chemiluminescent (ECL) labeled probes [20] , biological fluorescence detection [7] , Northern blot [8] , enzyme-linked immunosorbent assay (ELISA) [1] , molecular beacon, etc. Invention of kits including basic items for diagnosis simplified pathogen detection with NASBA.…”

Section: Development Of Nasbamentioning

confidence: 99%

“…Targets range from HIV to all kinds of microorganism monitored by quantification or qualification of pathogens by real-time NASBA. Additionally, there are integrated microfluidic NASBA technology [34] , immune NASBA [1] , multiplex NASBA [18] and [32] , etc. During the period of 2001-2010, the research and application of NASBA reached a climax, such as detection of microbial species, integrated methods and the development of NASBA, such as multiplex NASBA.…”

Section: Development Of Nasbamentioning

confidence: 99%

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Nucleic Acid Sequence-Based Amplification in Diagnosis of Disease Pathogens

2017

JAAID

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Section: Development Of Nasbamentioning

confidence: 98%

Section: Development Of Nasbamentioning

confidence: 99%

Section: Development Of Nasbamentioning

confidence: 99%

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Nucleic Acid Sequence-Based Amplification in Diagnosis of Disease Pathogens

2017

JAAID

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“…The dual recognition in combination with a ligation reaction ensures specificity of detection. After that, the circular padlock probe serves as template for the polymerase, which continuously elongates the product and displaces the generated strand [35]. It is also possible to use further primers binding the generated product and thereby producing hyper branching structures.…”

Section: Rca: Rolling Circle Amplificationmentioning

confidence: 99%

“…The reaction requires a thermal cycling instrumentation, space and considerable expertise [35]. Therefore, isothermal reactions for the target specific amplification are alternative and valuable methods for the simplification of diagnostic devices.…”

Section: Introductionmentioning

confidence: 99%

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Tröger¹,

Niemann²,

Gärtig³

et al. 2015

J Nanomed Nanotechnol

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Nucleic acid amplification technologies (NAATs) offer the most sensitive tests in the clinical laboratory. These techniques are used as a powerful tool for screening and diagnosis of infectious diseases. Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results.However, current molecular diagnostic approaches generally need manual analysis by qualified and experienced personal which is a highly complex, time-consuming and labor-intensive task. Thus, the demand for simpler, miniaturized systems and assays for pathogen detection is steadily increasing. Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis.In this article, we review several isothermal amplification methods and their implementation in microsystems in relation to quantification of nucleic acids. miniaturized systems can be diverse. The majority of publications are focussed on clinical diagnostics including foodborne organisms for environmental monitoring [10][11][12]. In case of an infectious disease, cultivation and phenotypic characterization are still the standard methods of microbiologists which need days up to weeks to obtain a diagnostic result. Hence, scientists started to make use of nucleic acid amplification methods to accelerate the analysis. The most widespread and well known technique for this purpose is the polymerase chain reaction (PCR) [10,13], which exploits the activity of the DNA polymerase. The method is based on a thermal cycling process consisting of repeated cycles of heating and cooling steps allowing for DNA melting, sequence specific primer binding and enzymatic amplification of the target nucleic acid. The quantitative assessment of target copies is possible by using a real-time PCR approach, where a concentration dependent signal (e.g. fluorescence) increases over time [14]. The latter enables the user to easily assess the pathogen load of patient samples [15][16][17][18]. Since the first example of a PCR on a miniaturized system in 1994 [19,20], numerous devices were presented, which integrate not just the amplification, but also sample preparation and detection steps [9,[21][22][23][24][25][26][27][28]. Just a few of those microsystems have reached the market so far, but some have shown a fascinating potential to replace the classical laboratory-oriented state-of-the art [29][30][31][32][33]. setups has been shown to obtain a similar result [34]. A smaller heat capacity allows for rapid changes in temperature beneficial for the PCR time as well as a higher parallelism of multiple genetic samples [34].However, a major drawback of the integration of PCR to microsystems is the necessity of sophisticated instrumentation. The reaction requires a thermal cycling instrumentation, space and considerable expertise [35]. Therefore, isothermal reactions for the ta...

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Molecular Microbiology

Potula

Tang

2016

Manual of Commercial Methods in Clinical Microbiology

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N 2 -fixation by Rhizobium-legume symbionts is of major ecological and agricultural importance, responsible for producing a substantial fraction of the biosphere's nitrogen. On the basis of 15 N-labelling studies, it had been generally accepted that ammonium is the sole secretion product of N 2 -fixation by the bacteroid and that the plant is responsible for assimilating it into amino acids. However, this paradigm has been challenged in a recent 15 N-labelling study showing that soybean bacteroids only secrete alanine. Hitherto, nitrogen secretion has only been assessed from in vitro 15 N-labelling studies of isolated bacteroids. We show that both ammonium and alanine are secreted by pea bacteroids. The in vitro partitioning between them will depend on whether the system is open or closed, as well as the ammonium concentration and bacteroid density. To overcome these limitations we identified and mutated the gene for alanine dehydrogenase (aldA) and demonstrate that AldA is the primary route for alanine synthesis in isolated bacteroids. Bacteroids of the aldA mutant fix nitrogen but only secrete ammonium at a significant rate, resulting in lower total nitrogen secretion. Peas inoculated with the aldA mutant are green and healthy, demonstrating that ammonium secretion by bacteroids can provide sufficient nitrogen for plant growth. However, plants inoculated with the mutant are reduced in biomass compared with those inoculated with the wild type. The labelling and plant growth studies suggest that alanine synthesis and secretion contributes to the efficiency of N 2fixation and therefore biomass accumulation.

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Diagnostic Devices for Isothermal Nucleic Acid Amplification

Cited by 131 publications

References 99 publications

Nucleic Acid Sequence-Based Amplification in Diagnosis of Disease Pathogens

Nucleic Acid Sequence-Based Amplification in Diagnosis of Disease Pathogens

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Molecular Microbiology

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