Diagnostic methodologies for the dengue viral infection

Correlation of clinical outcome with laboratory parameters in children with dengue infection: An appraisal from a tertiary children’s hospital in India

“…Many commercial assays are currently available for the detection of dengue NS1 antigen. 8,9,10 Among these tests, the Panbio early dengue NS1 capture ELISA has been shown to have an overall sensitivity of 60.4 to 66% and a specificity of 97.9 to 99% 8 . It has also been shown that NS1 rapid antigen detection tests (SD Diagnostics, Bioline, South Korea) have a sensitivity of 81.6% and a specificity of 92% .…”

Dengue Reemergence: The Challenges Ahead

“…Timely, efficient and accurate diagnosis of Dengue is of primary importance for outbreak control, pathogenesis, academic research, vaccine development, surveillance activities, clinical trials and clinical care [47]. Clinical care involves early detection of dengue infection, case confirmation and differential diagnosis with other infectious diseases.…”

“…Clinical care involves early detection of dengue infection, case confirmation and differential diagnosis with other infectious diseases. The most commonly used diagnostic techniques include: virus in cell culture, viral nucleic acid (molecular methods), dengue virus antigens/specific antibodies (serological tests) and hematological test (platelet and hematocrit value) [47]. For early stages, virus isolation, genome detection or antigen identification may each be used for diagnosis verses serology at acute phase.…”

“…antibodies, most of them have been questioned or unknown because proper validation studies have not been performed and the results not yet publicly declared [47]. The NS1 antigen assay (serological diagnosis) and ELISA are the best new alternatives for antibody diagnosis due to their high degrees of specificity and sensitivity [47].…”

“…antibodies, most of them have been questioned or unknown because proper validation studies have not been performed and the results not yet publicly declared [47]. The NS1 antigen assay (serological diagnosis) and ELISA are the best new alternatives for antibody diagnosis due to their high degrees of specificity and sensitivity [47]. However, the clinical diagnosis in the absence of laboratory confirmation is often unreliable but the cross-sectional serologic surveys have the potential to shed light on the broader population burden of DENV without these biases though the serologic cross-reactions among antibody-based assays for Flaviviruses can limit the reliability of such studies in the absence of confirmatory testing [48].…”