Diagnostic performance study of an antigen microarray for the detection of antiphospholipid antibodies in human serum

Schindler, Alan M.; Bleher, Oliver; Thaler, Markus; Kocot, Carmen; Steigerwald, Udo; Proll, Günther; Gauglitz, Günter; Luppa, Peter B.

doi:10.1515/cclm-2014-0569

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Sensitive and accurate biosensing systems for these biomarkers can not only significantly aid in the early diagnosis and clinical management of AIDs but also help to establish therapeutic strategies [ 4 ]. Biosensors for the detection of autoantibodies specific to AIDs, including antiphospholipid syndrome (APS) [ 91 , 92 , 93 , 94 ], rheumatoid arthritis (RA) [ 95 , 96 , 97 , 98 , 99 ], systemic lupus erythematosus (SLE) [ 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ], multiple sclerosis (MS) [ 108 , 109 , 110 , 111 ], and celiac disease (CD) [ 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 ], are now constructed based on diverse techniques with many advantages, including high sensitivity and easy operation.…”

Section: Potential Applications Of Antibody Biosensing For Clinicamentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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Section: Potential Applications Of Antibody Biosensing For Clinicamentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Immunosensors for Biomarker Detection in Autoimmune Diseases

Zhang

Zambrano

Lin

et al. 2016

Arch. Immunol. Ther. Exp.

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Autoimmune diseases occur when the immune system generates proinflammatory molecules and autoantibodies that mistakenly attack their own body. Traditional diagnosis of autoimmune disease is primarily based on physician assessment combined with core laboratory tests. However, these tests are not sensitive enough to detect early molecular events, and quite often, it is too late to control these autoimmune diseases and reverse tissue damage when conventional tests show positivity for disease. It is fortunate that during the past decade, research in nanotechnology has provided enormous opportunities for the development of ultrasensitive biosensors in detecting early biomarkers with high sensitivity. Biosensors consist of a biorecognition element and a transducer which are able to facilitate an accurate detection of proinflammatory molecules, autoantibodies and other disease-causing molecules. Apparently, novel biosensors could be superior to traditional metrics in assessing the drug efficacy in clinical trials, especially when specific biomarkers are indicative of the pathogenesis of disease. Furthermore, the portability of a biosensor enables the development of point-of-care devices. In this review, various types of biomolecule sensing systems, including electrochemical, optical and mechanical sensors, and their applications and future potentials in autoimmune disease treatment were discussed.

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Through the looking-glass - Recent developments in reflectometry open new possibilities for biosensor applications

Fechner

Gauglitz²,

Proll³

2022

TrAC Trends in Analytical Chemistry

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Diagnostic performance study of an antigen microarray for the detection of antiphospholipid antibodies in human serum

Abstract: Multiplexed determination of serological parameters has a great potential. We have shown that our biosensor is capable of detecting four different APS relevant antibodies in parallel exhibiting a sensitivity and specificity comparable to existing ELISA methods.

Cited by 3 publications

References 22 publications

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Immunosensors for Biomarker Detection in Autoimmune Diseases

Through the looking-glass - Recent developments in reflectometry open new possibilities for biosensor applications

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