Diagnostic strategy for occult hepatitis B virus infection

Ocaña, Sara

doi:10.3748/wjg.v17.i12.1553

Cited by 76 publications

(69 citation statements)

References 21 publications

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“…The prevalence of chronic HBV infection and availability of resources are major determinants of the screening methods used for HBV. For example, HBsAg with anti-HBc (total) and/or NAT is used for screening in resource-rich areas with a low prevalence of chronic HBV infection (208,233). A good proportion of blood donors with occult hepatitis B virus infection have anti-HBc as the only serological marker of HBV infection (129,242).…”

Section: Methods For Diagnosis Of Occult Hbv Infectionmentioning

confidence: 99%

Molecular Mechanisms Underlying Occult Hepatitis B Virus Infection

2012

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SUMMARY Chronic hepatitis B virus (HBV) infection is a complex clinical entity frequently associated with cirrhosis and hepatocellular carcinoma (HCC). The persistence of HBV genomes in the absence of detectable surface antigenemia is termed occult HBV infection. Mutations in the surface gene rendering HBsAg undetectable by commercial assays and inhibition of HBV by suppression of viral replication and viral proteins represent two fundamentally different mechanisms that lead to occult HBV infections. The molecular mechanisms underlying occult HBV infections, including recently identified mechanisms associated with the suppression of HBV replication and inhibition of HBV proteins, are reviewed in detail. The availability of highly sensitive molecular methods has led to increased detection of occult HBV infections in various clinical settings. The clinical relevance of occult HBV infection and the utility of appropriate diagnostic methods to detect occult HBV infection are discussed. The need for specific guidelines on the diagnosis and management of occult HBV infection is being increasingly recognized; the aspects of mechanistic studies that warrant further investigation are discussed in the final section.

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Section: Methods For Diagnosis Of Occult Hbv Infectionmentioning

confidence: 99%

Molecular Mechanisms Underlying Occult Hepatitis B Virus Infection

2012

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“…A prevalência do VHB é definida pelos testes realizados entre os pacientes; entretanto, a aproximação diagnóstica apresenta-se inadequada, pois a descoberta da IOB requer ensaios de alta sensibilidade e especificidade, com um limite de detecção < 10 UI/mL para VHB-DNA e < 0,1 mg/mL para HBsAg 69 .…”

Section: Introductionunclassified

Infecção oculta pelo vírus da hepatite B em comunidade amazônica submetida a intenso fluxo migratório, estado do Pará, Brasil

Nunes¹,

Soares²,

Sarmento³

et al. 2017

Revista Pan-Amazônica de Saúde

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The ones anti-HBc total+ isolated were selected, and from these the HBV DNA was investigated to identify OBI presence. RESULTS: In relation to HBV, the study showed 0.5% of carriers, 8.3% with a previous infection profile, 1.1% with a previous or current infection profile, and 33.6% with a vaccine response, characterizing that municipality as low endemicity for this virus. HBV DNA was detected in 66.7% of the HBsAg+ samples, with viral load between < 55 IU/mL and > 38,000 IU/mL; 69.2% of these samples were A1 subgenotype, 15.4% F2, and 15.4% F4. Of the total, 1.1% were samples from individuals anti-HBc total+ isolated whose molecular biology tests showed that 9.3% had HBV DNA, with viral load between < 15 IU/mL and 48 IU/mL. CONCLUSION: The findings of this study indicated the presence of OBI carriers in 0.1% of the Juruti population. The low prevalence of OBI found is probably related to the low endemicity of HBV in this population.

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“…However, a qualitative assay was optimized for the detection of HBV DNA, which can be very useful for the identification of HBV occult infection, genotypes and mutants (Ocana et al, 2011;Said, 2011;Chakravarty, 2011;Guirgis et al, 2010;Valsamakis, 2007). For these reasons, the present study will be very useful for clinical studies regarding HBV diagnosis and the monitoring of HBV treatment.…”

Section: Discussionmentioning

confidence: 99%

A comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples

Portilho

Martins

Lampe

et al. 2012

Journal of Medical Microbiology

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The objective of the present study was to evaluate four commercial DNA extraction methods and three PCR protocols for hepatitis B virus (HBV) detection in artificially contaminated oral fluid samples. The extraction protocols were selected based on ease of use and cost, and were also compared with respect to sensitivity and cost. Prior PCR optimization was conducted, in which the sample volume for DNA extraction and the concentrations of DNA and Taq DNA polymerase in the PCR were adjusted. One-round PCR, used to amplify the core region of the HBV genome, achieved high levels of sensitivity in comparison with nested and semi-nested PCR experiments that were designed for the amplification of HBV surface protein genes. Of the four extraction protocols evaluated, the RTP DNA/RNA Virus Mini kit and the QIAamp DNA Mini kit gave the highest recovery rates, presenting 20 copies of HBV DNA ml "1 as the limit of detection. These results suggest that HBV DNA can be detected from oral fluid samples but that the optimization of the PCR assays and the choice of extraction methods must be determined by laboratories before the implementation of this method in routine diagnostics.

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Diagnostic strategy for occult hepatitis B virus infection

Cited by 76 publications

References 21 publications

Molecular Mechanisms Underlying Occult Hepatitis B Virus Infection

Molecular Mechanisms Underlying Occult Hepatitis B Virus Infection

Infecção oculta pelo vírus da hepatite B em comunidade amazônica submetida a intenso fluxo migratório, estado do Pará, Brasil

A comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples

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