Diagnostic Techniques for COVID-19: A Mini-review of Early Diagnostic Methods

Dong, Gaopan; Guo, Xin; Sun, Yingai; Zhang, Zheng; Du, Lüpei; Li, Minyong

doi:10.1007/s41664-021-00198-5

Cited by 14 publications

(10 citation statements)

References 109 publications

(161 reference statements)

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“…In addition, enzyme-linked immunosorbent assay (ELISA) is another immunological detection technique for the detection of SARS-CoV-2 virus based on the specifical combination between viral antigen and antibody [ 51 ]. ELISA includes three detection methods, namely direct ELISA, indirect ELISA, and sandwich ELISA.…”

Section: Sars-cov-2 Infection Mechanism and Diagnostic Methodsmentioning

confidence: 99%

Visual Detection of COVID-19 from Materials Aspect

Wang

Meng

et al. 2022

Adv. Fiber Mater.

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In the recent COVID-19 pandemic, World Health Organization emphasized that early detection is an effective strategy to reduce the spread of SARS-CoV-2 viruses. Several diagnostic methods, such as reverse transcription-polymerase chain reaction (RT-PCR) and lateral flow immunoassay (LFIA), have been applied based on the mechanism of specific recognition and binding of the probes to viruses or viral antigens. Although the remarkable progress, these methods still suffer from inadequate cellular materials or errors in the detection and sampling procedure of nasopharyngeal/oropharyngeal swab collection. Therefore, developing accurate, ultrafast, and visualized detection calls for more advanced materials and technology urgently to fight against the epidemic. In this review, we first summarize the current methodologies for SARS-CoV-2 diagnosis. Then, recent representative examples are introduced based on various output signals (e.g., colorimetric, fluorometric, electronic, acoustic). Finally, we discuss the limitations of the methods and provide our perspectives on priorities for future test development. Graphical Abstract

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Section: Sars-cov-2 Infection Mechanism and Diagnostic Methodsmentioning

confidence: 99%

Visual Detection of COVID-19 from Materials Aspect

Wang

Meng

et al. 2022

Adv. Fiber Mater.

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“…The leaves of both Brassica napus L. infected and normal plants were cut for mRNA measurement. Total RNA was isolated using TRIzol (Invitrogen, USA) and then treated with DNase I to eliminate DNA contamination . One microgram of total RNA was then used for cDNA synthesis using the SuperScript III First-Strand Synthesis System (Invitrogen, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated using TRIzol (Invitrogen, USA) and then treated with DNase I to eliminate DNA contamination. 26 One microgram of total RNA was then used for cDNA synthesis using the SuperScript III First-Strand Synthesis System (Invitrogen, USA). qRT-PCR was performed with an ABI 7500 Real-Time PCR System (Bio-Rad, USA) using the iTaq Universal SYBR Green supermix (Bio-Rad, USA) and the primers (F, GAGGCCACCTTGTTACGCA; R, AGCCTGCGGTCATCAACTG).…”

Section: ■ Introductionmentioning

confidence: 99%

Diagnosis of Bacterial Plant Diseases via a Nitroreductase-Activated Fluorescent Sensor

Guo

Wang

et al. 2022

Anal. Chem.

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Plant diseases caused by bacteria have become one of the serious problems that threaten human food security, which led to the remarkable reduction of agricultural yields and economic loss. Nitroreductase (NTR), as an important biomarker, is highly expressed in bacteria, and the level of NTR is closely related to the progression of pathogen infection. Therefore, the design of smallmolecule fluorescent sensors targeting NTR is of great significance for the detection and diagnosis of plant pathogenic bacteria. In this study, a new fluorescent sensor targeting NTR was discovered and then successfully applied to the imaging of zebraf ish and pathogenic bacteria. Most importantly, the developed sensor achieved the real-time diagnosis of Brassica napus L. infected with bacteria, which provides a promising tool for examining the temporal and spatial infection of plant pathogens in precision agriculture.

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“…To reduce the transmission of SARS‐CoV‐2, accurate viral detection is an indispensable step before the isolation and treatment of positive individuals. At present, the main principle of detection is to analyze the nucleic acid of the virus, 2 by which quantitative real‐time polymerase chain reaction with reverse transcription (RT‐qPCR) is leveraged as the gold‐standard method for SARS‐CoV‐2 detection due to its high accuracy and fidelity 2 . But it is time‐consuming, expensive, and relies on laboratory instruments and specialists, limiting its wide use in low‐resource areas.…”

Section: Figurementioning

confidence: 99%