Diagnostic testing for SARS-CoV-2

Boodman, Carl; Lagacé-Wiens, Philippe; Bullard, Jared

doi:10.1503/cmaj.200858

Cited by 12 publications

(10 citation statements)

References 4 publications

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“…These assays measure acute infection, detecting viral RNA in respiratory samples, and are essential for individuals who require healthcare by tracing the infection in the population and reducing viral transmission. In contrast to molecular tests, serology should not be used to diagnose acute SARS-CoV-2 infection, and only on rare occasions might it be useful as an adjunct diagnostic test [1].…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

Matusali

Colavita

Lapa

et al. 2021

Viruses

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SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In the present study, 662 serum samples collected from February 2020 to January 2021 from acute and convalescent COVID-19 patients were tested to determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) for live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, and IgM directed against different viral antigens were measured by high-throughput automated platforms. We observed higher levels of NAbs in elderly (>60 years old) individuals and in patients presenting acute respiratory distress syndrome. SARS-CoV-2 NAbs develop as soon as five days from symptom onset and, despite a decline after the second month, persist for over 11 months, showing variable dynamics. Through correlation and receiver operating characteristic (ROC) curve analysis, we set up a testing algorithm, suitable for the laboratory workload, by establishing an optimal cutoff value of anti-SARS-CoV-2 IgG for convalescent plasma donors to exclude from MNT samples foreseen to have low/negative NAb titers and ineligible for plasma donation. Overall, MNT, although cumbersome and not suitable for routine testing of large sample sizes, remains the reference tool for the assessment of antibody-mediated immunity after SARS-CoV-2 infection. Smart testing algorithms may optimize the laboratory workflow to monitor antibody-mediated protection in COVID-19 patients, plasma donors, and vaccinated individuals.

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Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

Matusali

Colavita

Lapa

et al. 2021

Viruses

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“…The clinical performance of diagnostic tests largely depends on the specimen type, time of collection, and the circumstances in which they are used. Currently, the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) recommend the collection of upper respiratory tract samples for the diagnosis of SARS-CoV-2 [ 31 , 32 ]. General procedures for sampling collection should be followed as the accuracy of the test largely depends on pre-analytic variables related to the specimen quality and the sampling timing during the acute phase of the disease [ 13 , 33 ].…”

Section: Which Tests Should Be Used and In What Scenarios?mentioning

confidence: 99%

Molecular accuracy vs antigenic speed: SARS-CoV-2 testing strategies

Fajardo

Perbolianachis

Ferreiro

et al. 2022

Current Opinion in Pharmacology

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The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has hit every corner of the world faster than any infectious disease ever known. In this context, rapid and accurate testing of positive cases are essential to follow the test-trace-isolate strategy (TETRIS), which has proven to be a key approach to constrain viral spread. Here, we discuss how to interpret and combine molecular or/and antigen-based detection methods for SARS-CoV-2 as well as when they should be used. Their application can be cleverly designed as an algorithm to prevent viral dissemination according to distinct epidemiological contexts within surveillance programs.

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“…Worid Health Organization (WHO) telah menyatakannya sebagai darurat kesehatan global pada 30 Januari 2020. 1,2,3,4,5 Pada 22 Januari 2020, total 571 kasus virus corona baru 2019 (COVID- 19) dilaporkan di 25 provinsi (kabupaten dan kota) di China. 3,4 China National Health Commission melaporkan rincian 17 kematian pertama hingga 22 Januari 2020.…”

Section: Pendahuluanunclassified

“…18 Penelitian Boodman C et al tahun 2020 mendapati bahwa pengujian untuk SARSCoV-2 bergantung pada reaksi berantai polimerase transkripsi terbalik (RT-PCR). 19 Penelitian Habibzadeh P et al tahun 2020 menjelaskan bahwa uji RT-PCR adalah uji laboratorium terpenting yang saat ini kami andalkan untuk diagnosis penyakit Coronavirus 2019 (COVID-19). 20 Penelitian Caruana G et al tahun 2020 metode nucleic acid amplification tests (NAATs) tetap menjadi standard diagnosis COVID-19 karena memiliki sensitivitas yang tinggi pada tahap awal penyakit.…”

Section: Bahasanunclassified

“…25 Hal ini juga sejalan dengan penelitian Zafar H tahun 2020 diantara beberapa pemeriksaan yang ada untuk diagnostik, pemeriksaan secara molekuler yaitu teknik polymerase chain reaction (PCR) yang paling banyak digunakan dan bisa diandalkan. 26 Sesuai dengan penelitian literaturliteratur yang direview, pemeriksaan PCR direkomendasikan dan dijadikan sebagai "gold standard" dalam mendiagnostik Coronavirus Disease 2019 (COVID- 19) dikarenakan sensitivitas dan spesifisitas yang tinggi dari pemeriksaan PCR, sejalan dengan penelitian Uhteg K et al tahun 2020 metode deteksi asam nukleat lebih spesifik dalam penegakkan diagnosis dibandingkan metode tradisional termasuk kultur sel. 13 Penelitian Tang YW et al tahun 2020 mendapatkan PCR menjadi pilihan utama karena memiliki keunggulan dalam pemeriksaan amplifikasi dan analisisnya.…”

Section: Bahasanunclassified

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Diagnostik Coronavirus Disease 2019 (COVID-19) dengan Pemeriksaan Laboratorium Mikrobiologi Klinik

Damo

Porotu’o

Rambert

et al. 2021

eBM

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The world is in fear of the pandemic Coronavirus Disease (COVID-19) which is so deadly, the World Health Organization (WHO) declared the world a global health emergency. The number of confirmed cases is still very high until now, even in Indonesia this pandemic is still not over. The transmission is so fast and wide, plus the current clinical manifestations of COVID-19 have varied, ranging from asymptomatic to severe. This literature review aims to determine the Diagnostic of COVID-19 with the most appropriate Clinical Microbiology Laboratory Examination and to find out the importance of Clinical Microbiology Laboratory examinations in COVID-19 Diagnostics. The method used is in the form of literature studies from several scientific sources that are accurate and valid regarding the Diagnostic of COVID-19 with Clinical Microbiology Laboratory Examination. The results showed that Polymerase Chain Reaction (PCR) is the gold standard in detecting COVID-19 although there are several factors that can affect the results of the PCR examination which then cause false negatives / positives. Conclusion Clinical Microbiology Laboratory Examination is very important and very helpful. Diagnostic COVID-19 in the Microbiology Laboratory can be done by using Rapid Diagnostic Test (RDT) Antigen, RDT Antibody, PCR, and Virus Culture. Diagnostic COVID-19 with the recommended Clinical Microbiology Laboratory Examination and the gold standard is PCR examination. However, there are also several factors that can affect the results of the PCR examination.Keywords: diagnostic, COVID-19, Clinical Microbiology Laboratory Abstrak: Dunia sedang dalam ketakutan dengan pandemi Coronavirus Disease (COVID-19) yang begitu mematikan, Organisasi Kesehatan Dunia (WHO) menyatakan dunia sebagai darurat kesehatan global. Jumlah kasus yang terkonfirmasi masih sangat tinggi hingga saat ini, bahkan di Indonesia pandemi ini masih belum berakhir. Penularannya begitu cepat dan luas, sertta manifestasi klinis COVID-19 yang bervariasi, mulai dari asimtomatik hingga parah. Tinjauan pustaka ini bertujuan untuk mengetahui Diagnostik COVID-19 dengan Pemeriksaan Laboratorium Mikrobiologi Klinik yang paling sesuai dan untuk mengetahui pentingnya pemeriksaan Laboratorium Mikrobiologi Klinik dalam Diagnostik COVID-19. Metode yang digunakan berupa studi pustaka dari beberapa sumber ilmiah yang akurat dan valid mengenai Diagnostik COVID-19 dengan Pemeriksaan Laboratorium Mikrobiologi Klinik. Hasil penelitian menunjukkan bahwa Polymerase Chain Reaction (PCR) merupakan gold standard dalam mendeteksi COVID-19 meskipun terdapat beberapa faktor yang dapat mempengaruhi hasil pemeriksaan PCR yang kemudian menimbulkan false negatif/positif. Kesimpulan Pemeriksaan Laboratorium Mikrobiologi Klinik sangat penting dan sangat membantu. Diagnostik COVID-19 di Laboratorium Mikrobiologi dapat dilakukan dengan menggunakan Antigen Rapid Diagnostic Test (RDT), Antibodi RDT, PCR, dan Kultur Virus. Diagnostik COVID-19 dengan Pemeriksaan Laboratorium Mikrobiologi Klinik yang direkomendasikan dan menjadi gold standard adalah PCR. Namun, ada juga beberapa faktor yang dapat mempengaruhi hasil pemeriksaan PCR.Kata Kunci: diagnostik, COVID-19, Laboratorium Mikrobiologi Klinik

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Diagnostic testing for SARS-CoV-2

Cited by 12 publications

References 4 publications

SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

Molecular accuracy vs antigenic speed: SARS-CoV-2 testing strategies

Diagnostik Coronavirus Disease 2019 (COVID-19) dengan Pemeriksaan Laboratorium Mikrobiologi Klinik

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