Diagnostic tools in the evaluation of patients with viral hepatitis undergoing liver transplantation

Leon, Roberto; Medina, Maria de; Schiff, Eugene R.

doi:10.1002/lt.500040114

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…The problem is known as the so-called “window period”. In addition, some patients receive immunosuppressive therapy and the effective antibody generation may not occur [ 43 , 44 ].…”

Section: Aptamer In the Diagnostics Of Viral Infectionsmentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

114

109

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Aptamers are in vitro selected DNA or RNA molecules that are capable of binding a wide range of nucleic and non-nucleic acid molecules with high affinity and specificity. They have been conducted through the process known as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). It serves to reach specificity and considerable affinity to target molecules, including those of viral origin, both proteins and nucleic acids. Properties of aptamers allow detecting virus infected cells or viruses themselves and make them competitive to monoclonal antibodies. Specific aptamers can be used to interfere in each stage of the viral replication cycle and also inhibit its penetration into cells. Many current studies have reported possible application of aptamers as a treatment or diagnostic tool in viral infections, e.g., HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus), HCV (Hepatitis C Virus), SARS (Severe Acute Respiratory Syndrome), H5N1 avian influenza and recently spread Ebola. This review presents current developments of using aptamers in the diagnostics and treatment of viral diseases.

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“…The problem is known as the so-called “window period”. In addition, some patients receive immunosuppressive therapy and the effective antibody generation may not occur [ 43 , 44 ].…”

Section: Aptamer In the Diagnostics Of Viral Infectionsmentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

114

109

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“…The ease of automation and cost-effectiveness made serologic assays the most practical tool in HCV diagnosis [21] . However, antibody detection exhibits many disadvantages including that; detection is limited during the early stages of infection, poor sensitivity (false negative) in hemodialysis patients, immunocompromised patients [22][23][24][25] , an abundance of false-positives [26] (because recovered patients may stay anti-HCV positive for years) and variability in accuracy between deferent commercial kits.…”

Section: Advantages and Limitation Of Serologic Assaysmentioning

confidence: 99%

Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis

Zaghloul

El‐Shahat

2014

WJH

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Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.

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“…Although clearance of HBeAg is typically followed by the appearance of antibody to HBeAg (anti-HBe), its development is not invariable, may not persist and adds little diagnostically. A variety of tests, qualita-tive and quantitative, are available for HBV DNA detection (3).…”

Section: Hepatitis B Acute Hepatitis Bmentioning

confidence: 99%