Diagnóstico molecular e frequência de anticorpos anti-Leishmania infantum chagasi em cães do município de Belém, Pará

Schwanke, Katiane; Silva, Aryane M. M. da; Pacheco, Agenor Sarraf; Bahia, Michele; Sílveira, Fernando Tobias; Scofield, Alessandra; Góes-Cavalcante, Gustavo

doi:10.1590/s0100-736x2014000300010

Cited by 10 publications

(6 citation statements)

References 21 publications

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“…Corroborando com outros estudos realizados no Estado que também detectaram cães reagentes para anticorpos anti-Leishmania spp. (6,26,27) . É importante o desenvolvimento de novas pesquisas na região, pois o agente etiológico, o vetor e o principal reservatório urbano (cão) do parasita estão presentes no ambiente que é propício tanto para a manutenção do agente como do vetor.…”

Section: Discussionunclassified

INFECÇÃO POR Toxoplasma gondii, Neospora caninum, Leishmania major E Trypanosoma cruzi EM CÃES DO ESTADO DO PARÁ

et al. 2019

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Resumo Realizou-se inquérito sorológico para Toxoplasma gondii, Neospora caninum, Leishmania spp. e Trypanosoma cruzi em cães dos municípios de Castanhal e Belém, no Estado do Pará, e estudou-se a associação entre os animais sororeagentes e fatores como sexo, idade, acesso a rua e procedência dos animais. Foram utilizados 269 cães de diferentes idades, sadios, provenientes de abrigos de animais, canil comercial, Centro de Controle de Zoonoses e domiciliados. Utilizou-se a reação de imunofluorescência indireta (RIFI), com lâminas sensibilizadas de acordo com os seus respectivos antígenos. Das 263 amostras, 100 foram reagentes, com 38,0% de positividade para Toxoplasma gondii, sendo os títulos prevalentes 16UI e 64UI, 15,97% de sororreagentes para anticorpos anti-N. caninum com maior soroprevalência para os títulos 25UI e 50UI. Na pesquisa de anticorpos anti-L. major, 3,42% foram sororreagentes com o título 40UI, sendo o mais prevalente. A pesquisa de anticorpos anti-T. cruzi revelou negatividade em todas as amostras. As variáveis procedência e faixa etária mostraram ser um fator de risco para os animais. Os animais de Belém apresentaram 2,6 vezes mais chances de infecção do que os animais de Castanhal. Os animais na faixa etária de três a sete anos apresentaram 2,27 vezes mais chances de serem sororreagentes do que os cães com até três anos de idade. Não houve associação estatística para as demais variáveis e protozoários pesquisados.

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Section: Discussionunclassified

INFECÇÃO POR Toxoplasma gondii, Neospora caninum, Leishmania major E Trypanosoma cruzi EM CÃES DO ESTADO DO PARÁ

et al. 2019

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“…Segundo Schwanke et al (2014), além da sensibilidade, a especificidade da RIFI pode ser influenciada pela natureza do antígeno.…”

Section: Discussionunclassified

Aspectos epidemiológicos da leishmaniose visceral canina na zona rural do semiárido paraibano e análise de técnicas de diagnóstico

et al. 2016

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ABSTRACT.-Silva R.B.S., Mendes R.S., Santana V.L., Souza H.C., Ramos C.P.S., Souza A.P., Andrade P. Visceral leishmaniasis is caused by Leishmania infantum in Brazil. It is a chronic disease and often a fatal zoonosis and considered as a serious worldwide public health problem and recognized by the World Health Organization as one of seventeen "Neglected Tropical Diseases". This study was conducted in 2012 in the county of Patos, in Paraíba State semiarid region. The main purposes were to estimate the prevalence and the risk factors of canine visceral leishmaniasis (CVL), and to evaluate the performance of serological tests. Serological prevalence was determined using three techniques (ELISA, IFA and DPP chromatographic immunoassay -Dual Path Platform). ELISA was the golden standard, since it is the confirmatory test for CVL as established by the Brazilian Ministry of Health. In total, 362 blood samples were collected and were considered positive if positive by two serological assays. Positive samples were tested by PCR and qPCR. An epidemiological questionnaire was applied to the dog owner to identify risk factors associated with CVL. The seroprevalence was 11.33% (41/362). Chromatographic immunoassay -DPP showed a sensitivity of 58% (95%, CI: 0.43 to 0.72) and specificity of 96% (95%, CI: 0.93-0.98) , with a Kappa index of 0.5. The IFA had a sensitivity of 85% (95%, CI: 0.72 to 0.94), specificity of 99% (95%, CI: 0.98 to 1) and Kappa value of 0.8. The risk factor identified in the multivariate analysis was gender (OR=2.15 [1.09 to 4.21]), male dogs having 2.15 times more chance of being infected. The increased likelihood can possibly be explained by the use of male dogs for hunting and as guard dogs, two factors that increase dog contact to infected sandflies. Due to the prevalence rates, it should be recommended control measures against canine visceral leishmaniasis. The results demonstrated that the Chromatographic immunoassay (DPP-Dual Path Platform) is not the best technique for screening positive animals due to its low sensitivity, suggesting its replacement by ELISA.

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“…Several studies in other Brazilian cities have described autochthonous cases of CL (Tolezano et al, 2007;Silva et al, 2011;Faria et al, 2017, Silva et al, 2018Rocha et al, 2018, Pinto et al, 2021. Furthermore, although animals from Belém have been diagnosed with leishmaniasis (antibody detection) in two other studies (Campos et al, 2017, Schwanke et al, 2014 neither study indicated that the animals were born in Belém and never lived or traveled to endemic areas, as described for the animals in this study. The study by Schwanke et al (2014) failed to amplify the L. infantum DNA in the samples of dogs tested, and its results are based on serological data.…”

Section: Discussionmentioning

confidence: 46%

First molecular description of autochthonous urban cases of canine visceral leishmaniasis in the city of Belém, Pará, Brazil

et al. 2023

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Leishmaniasis is an anthropozoonosis transmitted by vectors, with dogs being the main domestic reservoirs. Brazil is one of the countries most affected by this disease, and it has been described in humans and dogs in every region in the country. In the northern region leishmaniasis cases in humans have been described in more than 100 municipalities in the State, including the capital, Belém. This study involves two cases of canine visceral leishmaniasis in which the animals developed clinical signs compatible with the disease in urban areas in Belém, the Pará state capital. The diagnosis was confirmed via polymerase chain reaction (PCR) to detect SSUr-rDNA and kDNA of Leishmania sp. and Leishmania infantum, respectively. In one of the cases the animal died and in the other the animal underwent treatment with medicines prescribed for dogs. Through this treatment, parasitemia in the second animal has been kept under control and is being monitored through molecular tests. Previously, no canine cases had been notified from urban neighborhoods in the city of Belém, but only on the island of Cotijuba, at a distance of 29 kilometers from the city. Cases of canine and human leishmaniasis have been recorded close to the capital, Belém, which has areas of conserved vegetation and where the presence of disease vectors has been described. Thus, as has been done in several other Brazilian cities, this study uses clinical and laboratory findings to confirm the presence of autochthonous cases of canine visceral leishmaniasis in the city of Belém.

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Diagnóstico molecular e frequência de anticorpos anti-Leishmania infantum chagasi em cães do município de Belém, Pará

Cited by 10 publications

References 21 publications

INFECÇÃO POR Toxoplasma gondii, Neospora caninum, Leishmania major E Trypanosoma cruzi EM CÃES DO ESTADO DO PARÁ

INFECÇÃO POR Toxoplasma gondii, Neospora caninum, Leishmania major E Trypanosoma cruzi EM CÃES DO ESTADO DO PARÁ

Aspectos epidemiológicos da leishmaniose visceral canina na zona rural do semiárido paraibano e análise de técnicas de diagnóstico

First molecular description of autochthonous urban cases of canine visceral leishmaniasis in the city of Belém, Pará, Brazil

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