Diallyl trisulfide (DATS) effectively induced apoptosis of postharvest diseasePenicillium expansum of citrus

Liu, Pu; Guo, Junhong; Liu, Huimin; Cheng, Yunjiang; Wang, Baoquan; Long, Chen; Deng, Boxun

doi:10.1007/bf03179207

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…4B, marked with arrows and letter v, respectively). Cell shrinkage and vacuole formation have been reported as characteristic features of apoptotic cell death in various studies of yeast, P. expansum, and A. nidulans (16,(21)(22)(23). Our observations are congruent with the hypothesis that starvation conditions, effected in this study by a low concentration of SPAF, induce apoptosis in R. oryzae.…”

Section: Discussionsupporting

confidence: 95%

Induction of Rhizopus oryzae Germination Under Starvation Using Host Metabolites Increases Spore Susceptibility to Heat Stress

Turgeman¹,

Kakongi²,

Schneider³

et al. 2014

Phytopathology®

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Sweetpotato is a nutritional source worldwide. Soft rot caused by Rhizopus spp. is a major limiting factor in the storage of produce, rendering it potentially unsafe for human consumption. In this study, Rhizopus oryzae was used to develop a concept of postharvest disease control by weakening the pathogen through induction of spore germination under starvation conditions. We isolated the sweetpotato active fractions (SPAFs) that induce spore germination and used them at a low dose to enhance spore weakening caused by starvation. Germination in SPAF at 1 mg/ml weakened the pathogen spores by delaying their ability to form colonies on rich media and by increasing their sensitivity to heat stress. The weakening effect was also supported by reduced metabolic activity, as detected by Alarmar Blue fluorescent dye assays. Spores incubated with SPAF at 1 mg/ml showed DNA fragmentation in some of their nuclei, as observed by TUNEL assay. In addition, these spores exhibited changes in ultrastructural morphology (i.e., shrinkage of germ tubes, nucleus deformation, and vacuole formation) which are hallmarks of programmed cell death. We suggest that induction of spore germination under starvation conditions increases their susceptibility to stress and, therefore, might be considered a new strategy for pathogen control.

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Section: Discussionsupporting

confidence: 95%

Induction of Rhizopus oryzae Germination Under Starvation Using Host Metabolites Increases Spore Susceptibility to Heat Stress

Turgeman¹,

Kakongi²,

Schneider³

et al. 2014

Phytopathology®

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“…DATS also has effective control of postharvest disease Penicillium expansum of citrus, the destructive stored-product pest ( Sitotroga cerealella ), the important foodborne pathogen ( Campylobacter jejuni ), etc. [ 19 , 23 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…DATS is considered to be a biological antibacterial agent that has inhibitory effects on Penicillium spp., Botrytis cinerea , Pseudomonas aeruginosa , Trametes hirsuta , Laetiporus sulphureus , Klebsiella pneumoniae , Campylobacter jejuni , etc. [ 19 , 20 , 21 , 22 , 23 , 24 ]. There were some reports that garlic juice was able to reduce disease severity in potato tuber late blight [ 25 ], and garlic crude extracts were found to have inhibitive effects on the growth of P. infestans [ 26 ], while the underlying mechanism of garlic extracts, juice, or its bioingredients against P. infestans is very limited.…”

Section: Introductionmentioning

confidence: 99%

Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight

Jian

Feng

Huang

et al. 2023

IJMS

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Potato late blight, caused by Phytophthora infestans, leads to a significant reduction in the yield and value of potato. Biocontrol displays great potential in the suppression of plant diseases. Diallyl trisulfide (DATS) is a well-known natural compound for biocontrol, although there is little information about it against potato late blight. In this study, DATS was found to be able to inhibit the hyphae growth of P. infestans, reduce its pathogenicity on detached potato leaves and tubers, and induce the overall resistance of potato tubers. DATS significantly increases catalase (CAT) activity of potato tubers, and it does not affect the levels of peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA). The transcriptome datasets show that totals of 607 and 60 significantly differentially expressed genes (DEGs) and miRNAs (DEMs) are detected. Twenty-one negatively regulated miRNA-mRNA interaction pairs are observed in the co-expression regulatory network, which are mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, and starch and sucrose metabolism based on the KEGG pathway. Our observations provide new insight into the role of DATS in biocontrol of potato late blight.

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“…Apart from their potential application in medicine for the production of antiradiation drugs [54], polysulfanes are of tremendous importance in agriculture owing to their possession of potent antimicrobial, insecticidal and larvicidal [55] properties that aid both actively growing plants and post-harvest crops in their defence against pests and diseases. In particular, diallyltrisulfide (DATS) and diallyltetrasulfide (DATTS) formed directly as breakdown products of allicin from garlic and also as steam distillates of garlic oil have been shown to be active against various pathogens like Penicillium expansum [56], which causes post harvest decay of many fruits and produces the carcinogenic mycotoxin, 'patulin'.…”

Section: F Polysulfanesmentioning

confidence: 99%

Sulfur and Sulfur Compounds in Plant Defence

Nwachukwu

Slusarenko

Gruhlke

2012

Natural Product Communications

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The multiplicity of chemical structures of sulfur containing compounds, influenced in part by the element's several oxidation states, directly results in diverse modes of action for sulfur-containing natural products synthesized as secondary metabolites in plants. Sulfur-containing natural products constitute a formidable wall of defence against a wide range of pathogens and pests. Steady progress in the development of new technologies have advanced research in this area, helping to uncover the role of such important plant defence molecules like endogenously-released elemental sulphur, but also deepening current understanding of other better-studied compounds like the glucosinolates. As studies continue in this area, it is becoming increasingly evident that sulfur and sulfur compounds play far more important roles in plant defence than perhaps previously suspected.

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Diallyl trisulfide (DATS) effectively induced apoptosis of postharvest diseasePenicillium expansum of citrus

Cited by 8 publications

References 23 publications

Induction of Rhizopus oryzae Germination Under Starvation Using Host Metabolites Increases Spore Susceptibility to Heat Stress

Induction of Rhizopus oryzae Germination Under Starvation Using Host Metabolites Increases Spore Susceptibility to Heat Stress

Integrative mRNA and microRNA Analysis Exploring the Inducing Effect and Mechanism of Diallyl Trisulfide (DATS) on Potato against Late Blight

Sulfur and Sulfur Compounds in Plant Defence

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