Diallyl Trisulfide Protects Rat Brain Tissue against the Damage Induced by Ischemia-Reperfusion through the Nrf2 Pathway

Abstract

Stroke is a public health problem due to its high mortality and disability rates; despite these, the pharmacological treatments are limited. Oxidative stress plays an important role in cerebral damage in stroke and the activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) confers protection against oxidative stress. Different compounds, such as diallyl trisulfide (DATS), have the ability to activate Nrf2. DATS protects against the damage induced in oxygen-glucose deprivation in neuronal cells; however, in in vivo models of cerebral ischemia, DATS has not been evaluated. Male Wistar rats were subjected to 1 h of ischemia and seven days of reperfusion and the protective effect of DATS was evaluated. DATS administration (IR + DATS) decreased the infarct area and brain damage in the striatum and cortex; improved neurological function; decreased malondialdehyde and metalloproteinase-9 levels; increased Nrf2 activation in the cortex and the expression of superoxide dismutase 1 (SOD1) in the nucleus, SOD2 and glutathione S-transferase (GST) in the striatum and cortex; and increased the activity of catalase (CAT) in the striatum and glutathione peroxidase (GPx) in the cortex. Our results demonstrate the protective effect of DATS in an in vivo model of cerebral ischemia that involves Nrf2 activation.

Effect of anhydrous betaine and hydrochloride betaine on growth performance, meat quality, postmortem glycolysis, and antioxidant capacity of broilers

“…To investigate the molecular mechanism by which muscle antioxidant capacity was regulated by betaine, the mRNA expressions of antioxidant genes were measured in the present study. The Nrf2 is an important transcription factor that plays a fundamental role in cellular defense against reactive free radicals and other oxidant species ( Silva-Islas et al., 2019 ). HO-1 , a downstream antioxidant gene of Nrf2 signaling pathway, is involved in the oxidative degradation of heme ( Loboda et al., 2016 ).…”

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps

“…Diallyl trisulfide (DATS), one of the major and biologically active components of garlic oil, exhibits a beneficial effect on the recovery of I/R injury. For example, it is suggested that DATS could be used for the treatment of I/R injury in brain tissues for its inhibitory effects on oxidative stress through Nrf2 activation ( Silva-Islas et al, 2019 ). Besides, DATS is also believed to own the ability for the promotion of angiogenesis ( Hayashida et al, 2017 ), which can be utilized for the protection of ischemic tissue in diabetic mice ( Yang et al, 2018 ) as well as the salvation of the ischemic limb ( Hsieh et al, 2019 ).…”

Activation of Nrf2 by Natural Bioactive Compounds: A Promising Approach for Stroke?

“…In rats receiving DATS decreased MDA levels were reported. Interestingly, Nrf2 induction increased in the cortex as well as SOD1 in the nucleus, SOD2 and glutathione S-transferase (GST) at both striatum and cortex levels [56]. SAC was able to exert cytoprotective effects, inducing Nrf2 and its downstream targets and suppressing JNK and p38 phosphorylation in cortical neurons exposed to OGD.…”

“…In rats receiving DATS decreased MDA levels were reported. Interestingly, Nrf2 induction increased in the cortex as well as SOD1 in the nucleus, SOD2 and glutathione S-transferase (GST) at both striatum and cortex levels [ 56 ].…”

“…Rhodiola plants possess both edible and medicinal value and are considered functional food [118]. Salidroside (C 14 H 20 O 7 ; CAS number 10338-51-9; Figure 11 (56)) is a constituent of Rhodiola. Salidroside exerted neuroprotection in a MCAO model.…”