Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

Electrochemical biosensor for the multiplexed detection of human papillomavirus genes

“…In this work, TMB was used as a chromogenic substrate for HRP. TMB is commonly used in ELISA assays and is oxidised to form a blue coloured product, which is known to be a single oxidised TMB complexed with a neutral TMB molecule (Kim et al, 2009). The oxidised TMB Fig.…”

Development of a sensitive SPR biosensor for C-reactive protein (CRP) using plasma-treated parylene-N film

“…Under the same conditions, the plasma-treated parylene-N film was observed to be far more effective for immobilization than the polystyrene surface used conventionally for the physical adsorption of proteins [13]. In this work, the optimal conditions of the plasma-treatment were estimated for the immobilization of proteins to the parylene-N film by using different plasma powers and treatment times using HRP with a neutral charge (pI ∼ = 7) as a model protein [20][21][22]. As shown in Fig.…”

“…After washing with 50 mM of phosphate-buffered saline (pH 7.0), the microplate was treated with 100 l of HRP solution in the concentration range from 1 to 100 g/ml for 1 h at 37 • C. After the incubation step, the microplate was washed with 1% Tween 20 in PBS using an automated washing machine from Molecular Devices Korea (Seoul, Korea). For the quantification of the immobilized HRP, the TMB solution was treated for 3 min and then the enzyme reaction of HRP with TMB was quenched by treatment of 100 l of 2 M sulfuric acid [20][21][22].…”

Improved detection limits of toxic biochemical species based on impedance measurements in electrochemical biosensors

“…Recently, the use of microelectrodes in electrochemical detection has also been receiving close attention in this field (Li et al, 2010;Bitziou et al, in press;Raina et al, 2010;Hu et al, 2010;Lupu et al, 2010;Antohe et al, 2009;Xu et al, 2009;Kim et al, 2009;Huffman and Venton, 2009;Lee et al, 2008;Zaouak et al, 2009;Qing et al, 2008;Xiang et al, 2007). Pioneering work was done on interdigitated array (IDA) electrodes by Sanderson and Anderson (1985) and several applications were reported (Chidsey et al, 1986;Fosdick et al, 1986;Feldman and Murray, 1986;Yang, 2008;Alocilja, 2008).…”