Diamond microelectrode arrays for in vitro neuronal recordings

McDonald, Matthew; Monaco, Antonina; Vahidpour, Farnoosh; Haenen, Ken; Giugliano, Michèle; Nesládek, Miloš

doi:10.1557/mrc.2017.62

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…Here carbon nanomaterials can offer a solution. Carbon allotropes used for transparent microelectrodes include boron‐doped diamond (2.1 Ω cm 2 , 50% transparent), carbon nanostructures (<0.7 Ω cm 2 , 40% transparent), and graphene (30–150 Ω cm 2 , >90% transparent). High transparency consistently comes at a cost to impedance.…”

Section: Introductionmentioning

confidence: 99%

Transparent Graphene/PEDOT:PSS Microelectrodes for Electro‐ and Optophysiology

Kshirsagar

Dickreuter

Mierzejewski

et al. 2018

Adv Materials Technologies

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Conventional opaque electrodes in microelectrode array (MEA) technology obstruct the view of cells in their immediate vicinity (e.g., ≈50 µm) from which the strongest extracellular action potentials are recorded. This limitation has been overcome by transparent graphene electrodes which allow for optical access essential for novel applications such as optogenetics and calcium imaging. Downscaling, necessary for high resolution single‐unit electrophysiological recordings, has been a significant challenge due to inferior electrochemical impedance and correspondingly lower signal‐to‐noise ratio. Here, the combination of graphene with the conductive polymer poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) as a transparent microelectrode material for in vitro MEAs is presented and their application with optical imaging and electrophysiology is demonstrated. Optimal graphene/PEDOT:PSS microelectrodes display transparencies of 84% over the visible spectrum and impedance magnitude of (166 ± 13) kΩ at 1 kHz. The balance of transparency and 1 kHz impedance can be tuned from ≈90% and 700 kΩ to 50% and 42 kΩ.

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Section: Introductionmentioning

confidence: 99%

Transparent Graphene/PEDOT:PSS Microelectrodes for Electro‐ and Optophysiology

Kshirsagar

Dickreuter

Mierzejewski

et al. 2018

Adv Materials Technologies

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“…A widely adopted strategy for the fabrication of multi-electrode arrays is based on growing doped diamond layers, in which, by varying the doping level, a diamond can be tuned from insulator (10 −6 S m −1 ) to a metal-like conductive material (10 4 S m −1 ). In diamond, dopant elements employed are nitrogen [ 116 ] or more often boron [ 32 , 42 , 52 , 65 , 66 ] in concentration up to 10 21 cm −3 . Both of them are working as acceptors.…”

Section: Detection Of Neurotransmitter Release and Action Potentials mentioning

confidence: 99%

Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes

Kühn¹,

Picollo

Carabelli

et al. 2020

Pflugers Arch - Eur J Physiol

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To understand the working principles of the nervous system is key to figure out its electrical activity and how this activity spreads along the neuronal network. It is therefore crucial to develop advanced techniques aimed to record in real time the electrical activity, from compartments of single neurons to populations of neurons, to understand how higher functions emerge from coordinated activity. To record from single neurons, a technique will be presented to fabricate patch pipettes able to seal on any membrane with a single glass type and whose shanks can be widened as desired. This dramatically reduces access resistance during whole-cell recording allowing fast intracellular and, if required, extracellular perfusion. To simultaneously record from many neurons, biocompatible probes will be described employing multi-electrodes made with novel technologies, based on diamond substrates. These probes also allow to synchronously record exocytosis and neuronal excitability and to stimulate neurons. Finally, to achieve even higher spatial resolution, it will be shown how voltage imaging, employing fast voltage-sensitive dyes and two-photon microscopy, is able to sample voltage oscillations in the brain spatially resolved and voltage changes in dendrites of single neurons at millisecond and micrometre resolution in awake animals.

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“…Even though diamond offers many benefits over other materials used for the fabrication of neuroelectrodes, the overall performance of flat electrodes made of nanocrystalline diamond does not exceed the one of conventional materials . This problem can be addressed by increasing the surface specific area.…”

Section: Introductionmentioning

confidence: 99%

Molecular Functionalization of Planar Nanocrystalline and Porous Nanostructured Diamond to Form an Interface with Newborn and Adult Neurons

Krůšek

Dittert

Smejkalová

et al. 2018

Physica Status Solidi (b)

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This article examines the ability of newly developed nanostructured porous boron‐doped diamond (BDD) to form an interface with neural cells and the role of molecular functionalization by a polymer on this interface. Due to its high stability, biocompatibility, and electrical properties, BDD is a promising material for construction of neuroelectrodes. Nanostructuring and an increase in the surface specific area can further improve the sensitivity and performance of such electrodes. Here, porous BDD prepared in a multistep diamond deposition on a porous template consisting of a polymer and electrospun SiO2 fibers is examined. This work shows that this new material is biocompatible and does not exhibit any cytotoxicity on fibroblast cell lines. Further, this work shows that porous BDD supports regeneration of newborn and adult neurons when functionalized with poly‐L‐lysine.

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Diamond microelectrode arrays for in vitro neuronal recordings

Abstract: Abstract

Cited by 17 publications

References 29 publications

Transparent Graphene/PEDOT:PSS Microelectrodes for Electro‐ and Optophysiology

Transparent Graphene/PEDOT:PSS Microelectrodes for Electro‐ and Optophysiology

Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes

Molecular Functionalization of Planar Nanocrystalline and Porous Nanostructured Diamond to Form an Interface with Newborn and Adult Neurons

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