Diapause Induction in the Stem Corn Borer, Sesamia nonagrioides (Lepidoptera: Noctuidae)

Eizaguirre, Matilde; Albajes, R.

doi:10.1127/entom.gen/17/1992/277

Cited by 67 publications

(57 citation statements)

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“…Plots were overplanted and thinned, obtaining a final density of ~70,000 plant ha −1 . The evaluations were performed under artificial infestation with MCB eggs obtained at the Misión Biológica de Galicia by rearing the insects as described by Eizaguirre and Albajes (1992), and Khan and Saxena (1997). Before flowering, five normal and competitive plants per plot were infested with ~40 MCB eggs placed between the stem and the sheath of a basal leaf.…”

Section: Methodsmentioning

confidence: 99%

QTL Mapping for Yield and Resistance against Mediterranean Corn Borer in Maize

et al. 2017

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Introduction: The Mediterranean corn borer (MCB), Sesamia nonagrioides, is a major pest of maize, Zea mays, in Mediterranean countries, inflicting significant kernel yield losses. For that reason, it necessary to know the genetic mechanisms that regulate the agronomic and resistance traits. A quantitative trait loci (QTL) mapping study for yield, resistance against MCB attack, and other relevant agronomic traits was performed using a recombinant inbred line (RIL) population derived from the cross A637 × A509 that is expected to segregate for yield, and ear, and stalk resistance to MCB. 171 RILs were evaluated in 2014 and 2015 at Pontevedra, Spain, along with the two parental inbreds A637 and A509 using a 13 × 14 single lattice design with two replications. A genetic map with 285 SNP markers was used for QTL analysis. Our objectives were to detect QTL for resistance to MCB and tolerance-related agronomic traits, to gain insights on the genetic relationship between resistance to MCB attack and yield, and to establish the best way for simultaneously improving yield and resistance to MCB.Results: Twelve significant QTL were detected for agronomic and resistance traits. QTL at bins 1.10 and 5.04 were especially interesting because the same allelic variant at these QTL simultaneously improved yield and insect resistance. In contrast, in the region 8.04–8.05, QTL showed opposite effects for yield and resistance. Several QTL for indexes which combine yield and resistance traits were found especially in the region 10.02–10.03.Conclusions: Selecting genotypes with the favorable allele of QTL on chromosome 5 (bin 5.01) will decrease tunnel length without affect yield, silking and plant height and QTL on the region 5.04 could be used to improve stalk resistance and yield simultaneously. An allele of QTL on bin 9.07 will increase ear resistance to MCB attack but it could produce later varieties while favorable allele in region 1.10 could improve ear and stalk resistance and yield without secondary negative effects. The region 8.03–8.05 mainly but also the region 10.02–10.03 and 5.04 may play an important role to elucidate the association between yield, other agronomic traits and MCB resistance.

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Section: Methodsmentioning

confidence: 99%

QTL Mapping for Yield and Resistance against Mediterranean Corn Borer in Maize

et al. 2017

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“…Larvae were reared on a semi-artificial diet [27]. The adults were supplied with a sugar solution (10%) and maintained at 21°C and high humidity (>60%) under a 16:8 h light: dark photoperiod.…”

Section: Methodsmentioning

confidence: 99%

Response of Last Instar Helicoverpa armígera Larvae to Bt Toxin Ingestion: Changes in the Development and in the CYP6AE14, CYP6B2 and CYP9A12 Gene Expression

et al. 2014

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Bt crops are able to produce Cry proteins, which were originally present in Bacillus thuringiensis bacteria. Although Bt maize is very efficient against corn borers, Spanish crops are also attacked by the earworm H. armigera, which is less susceptible to Bt maize. Many mechanisms could be involved in this low susceptibility to the toxin, including the insect's metabolic resistance to toxins due to cytochrome P450 monooxygenases. This paper examines the response of last instar H. armigera larvae to feeding on a diet with Bt and non-Bt maize leaves in larval development and in the gene expression of three P450 cytochromes: CYP6AE14, CYP6B2 and CYP9A12. Larvae fed on sublethal amounts of the Bt toxin showed reduced food ingestion and reduced growth and weight, preventing most of them from achieving the critical weight and pupating; additionally, after feeding for one day on the Bt diet the larvae showed a slight increase in juvenile hormone II in the hemolymp. Larvae fed on the non-Bt diet showed the highest CYP6AE14, CYP6B2 and CYP9A12 expression one day after feeding on the non-Bt diet, and just two days later the expression decreased abruptly, a finding probably related to the developmental programme of the last instar. Moreover, although the response of P450 genes to plant allelochemicals and xenobiotics has been related in general to overexpression in the resistant insect, or induction of the genes when feeding takes place, the expression of the three genes studied was suppressed in the larvae feeding on the Bt toxin. The unexpected inhibitory effect of the Cry1Ab toxin in the P450 genes of H. armigera larvae should be thoroughly studied to determine whether this response is somehow related to the low susceptibility of the species to the Bt toxin.

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“…S. nonagrioides cultures were established from insects collected in central Catalonia and reared on semi-artificial diets at 25°C [48]. Neonate larvae were divided into two groups, submitted to long-day (LD: 16 h light, 8 h dark) or short-day (SD: 12 h light, 12 h dark) photoperiod conditions and checked periodically.…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

2012

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BackgroundSesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter diapause and undergo up to 12 molts before dying or pupating. To better understand the mechanism of larval development and diapause, we identified proteins with different expressions in the sixth instar of diapausing and non-diapausing larvae.ResultsA total of 52 differentially regulated proteins were detected in the hemolymph of the diapausing or non-diapausing larvae at the beginning or end of the sixth instar. From these proteins, 11 were identified by mass spectrometry (MALDI-TOF MS or MALDI-TOF/TOF MS/MS): 5 were upregulated in the hemolymph of non-diapausing larvae and 6 in the hemolymph of the diapausing larvae. Interestingly, some proteins were expressed only in non-diapausing larvae but none was expressed only in the hemolymph of diapausing larvae. The possible functions of some of these proteins related to diapause maintenance or to larval-pupal metamorphosis are discussed.ConclusionsThe 2-DE proteomic map of S. nonagrioides hemolymph shows differential protein expression in diapausing and non-diapausing larvae. Some proteins that showed higher expression in the diapausing larvae at the end of the sixth instar could be involved in JH level maintenance thus in the diapause status maintenance. On the contrary, other proteins that showed the highest expression or that were expressed only in the non-diapausing larvae could be involved in larval-pupal metamorphosis.

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Diapause Induction in the Stem Corn Borer, Sesamia nonagrioides (Lepidoptera: Noctuidae)

Cited by 67 publications

References 0 publications

QTL Mapping for Yield and Resistance against Mediterranean Corn Borer in Maize

QTL Mapping for Yield and Resistance against Mediterranean Corn Borer in Maize

Response of Last Instar Helicoverpa armígera Larvae to Bt Toxin Ingestion: Changes in the Development and in the CYP6AE14, CYP6B2 and CYP9A12 Gene Expression

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

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