Diarylureas: New Promising Small Molecules against Streptococcus mutans for the Treatment of Dental Caries

Iacopetta, Domenico; Ceramella, Jessica; Catalano, Alessia; D’Amato, Assunta; Lauria, Graziantonio; Saturnino, Carmela; Andreu, Inmaculada; Longo, Pasquale; Sinicropi, Maria Stefania

doi:10.3390/antibiotics12010112

Cited by 13 publications

(5 citation statements)

References 102 publications

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“…S. mutans is a bacterium found in the oral cavity that is strongly linked to the development of dental caries. The bacterium can adhere to and form a biofilm on the surfaces of teeth, acidifying the environment and leading to enamel demineralization [ 21 ]. In order to check if the studied tannins—PGG, dGVG, and b-dGVG—have antibacterial potential against S. mutans , two parameters were investigated: MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Plant Polyphenols Belonging to the Tannins against Streptococcus mutans—Potential against Dental Caries

Czerkas,

Olchowik-Grabarek,

Łomanowska

et al. 2024

Molecules

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Dental caries (DC) is the most common oral pathology. The main bacteria responsible for DC is Streptococcus mutans. One of the strategies that can decrease or eliminate the risk of DC development is using compounds that will inhibit both the growth and virulence factors of S. mutans. Tannins are plant polyphenols that have strong antibacterial activity. The purpose of this study was to assess the antibacterial activity of three tannins against S. mutans. In this investigation, microbiological tests (MIC and MBC) and physicochemical techniques like the fluorescence measurements of tannins’ interaction with S. mutans cell membrane and membrane proteins, zeta potential, and thermodynamic analyses were used to obtain knowledge about the antibacterial potential of the investigated compounds against S. mutans as well as about the mechanisms associated with antibacterial activity. The obtained results demonstrate that the used compounds exhibit high antibacterial activity against S. mutans. The mechanisms of their antibacterial activity are linked to the strong change in the S. mutans membrane fluidity and potential, and to their interaction with membrane proteins that can result in great disturbance of bacterial physiology and ultimately the inhibition of bacterial growth, triggering their death. Therefore, it can be concluded that the investigated compounds can be potentially used as natural factors in the prevention of dental caries.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Plant Polyphenols Belonging to the Tannins against Streptococcus mutans—Potential against Dental Caries

Czerkas,

Olchowik-Grabarek,

Łomanowska

et al. 2024

Molecules

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“…Streptococcus mutans is a facultative anaerobic Gram-positive recognized as the one of the main bacteria that can cause caries. 5,6 Streptococcus mutans is able to metabolize sucrose to produce acid and, through the enzyme glucosyltransferase (Gtf), convert sucrose into extracellular polymer glucan, which promotes biofilm formation through cellular attachment to the tooth surface and other oral microorganisms. Streptococcus mutans can metabolize polysaccharides into organic acids (acidogenicity) and can survive in acidic environments with low pH conditions (acidurity).…”

Section: Introductionmentioning

confidence: 99%

The effect of silver-ion water on the growth of Streptococcus mutans

Wicaksono,

Tedjosasongko,

Dewi

et al. 2024

IJDM

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Background: Caries is a multifactorial disease that often occurs in the oral cavity and can be caused by Streptococcus mutans. Various ways have been done to reduce the number of Streptococcus mutans, one of them is by exposing them to a material agent such as silver-ion water. Silver-ion water has been recognized as an effective material for inhibiting several pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli. Thus, there is a possibility that silver-ion water can be used to reduce the number of bacteria that cause dental caries, such as Streptococcus mutans. Purpose: To determine the effect of silver ion water on the growth of Streptococcus mutans. Methods: This research is an experimental laboratory study (in vitro) with a control group post-test-only design. Streptococcus mutans were exposed to 0-15 ppm silver-ion water on Brain Heart Infusion Broth (BHIB) media, then Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were observed. Results: Silver-ion water with a concentration of 12.5 ppm was determined as MIC, and silver-ion water with a concentration of 15 ppm as MBC. Conclusion: Silver ion water is adequate as an antibacterial material in inhibiting (12.5 ppm) and killing (15 ppm) Streptococcus mutans.

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“…Thus, there is a growing need to develop new anticaries agents that can effectively inhibit bacterial growth and biofilm formation without causing resistance. Small molecule drugs, due to their stability, membrane permeability, and low toxicity, are promising candidates . Many naturally occurring drugs such as terpenoids, essential oils, and flavonoids have been shown to disrupt biofilm assembly and/or reduce EPS synthesis. − Trans- cinnamaldehyde (CA), an α,β-unsaturated aromatic aldehyde, is the main component of cinnamon essential oil in China .…”

Section: Introductionmentioning

confidence: 99%

Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Zhang,

Mu,

Wang

et al. 2024

J. Proteome Res.

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Dental caries is a chronic oral infectious disease, and Streptococcus mutans (S. mutans) plays an important role in the formation of dental caries. Trans-cinnamaldehyde (CA) exhibits broad-spectrum antibacterial activity; however, its target and mechanism of action of CA on S. mutans needs to be further explored. In this study, it was verified that CA could inhibit the growth and biofilm formation of S. mutans. Further proteomic analysis identified 33, 55, and 78 differentially expressed proteins (DEPs) in S. mutans treated with CA for 1, 2, and 4 h, respectively. Bioinformatics analysis showed that CA interfered with carbohydrate metabolism, glycolysis, pyruvate metabolism, and the TCA cycle, as well as amino acid metabolism of S. mutans. Protein interactions suggested that pyruvate dehydrogenase (PDH) plays an important role in the antibacterial effect of CA. Moreover, the upstream and downstream pathways related to PDH were verified by various assays, and the results proved that CA not only suppressed the glucose and sucrose consumption and inhibited glucosyltransferase (GTF) and lactate dehydrogenase (LDH) activities but also decreased the ATP production. Interestingly, the protein interaction, qRT-PCR, and molecular docking analysis showed that PDH might be the target of CA to fight S. mutans. In summary, the study shows that CA interferes with the carbohydrate metabolism of bacteria by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle via binding to PDH, which verifies that PDH is a potential target for the development of new drugs against S. mutans.

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Diarylureas: New Promising Small Molecules against Streptococcus mutans for the Treatment of Dental Caries

Cited by 13 publications

References 102 publications

Antibacterial Activity of Plant Polyphenols Belonging to the Tannins against Streptococcus mutans—Potential against Dental Caries

Antibacterial Activity of Plant Polyphenols Belonging to the Tannins against Streptococcus mutans—Potential against Dental Caries

The effect of silver-ion water on the growth of Streptococcus mutans

Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

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