Diastolic dysfunction in spontaneous type 2 diabetes rhesus monkeys: a study using echocardiography and magnetic resonance imaging

Qian, Can; Gong, Li; Yang, Zunyuan; Chen, Wei; Chen, Yushu; Xu, Ziqian; Wu, Bing; Chungui, Tang; Gao, Fabao; Zeng, Wen

doi:10.1186/s12872-015-0046-9

Cited by 27 publications

(24 citation statements)

References 54 publications

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“…In comparison with studies in rodent models (Table 1 ), obesity or metabolic syndrome (such as high fat diet alone or in combination with experimental diabetes) induced HFpEF has been sparsely studied in large animal models. Diastolic dysfunction, albeit in a variable degree, was however documented recently in type 2 DM rhesus monkeys (Table 2 ), although LV histology was performed in only one diseased animal [ 45 ]. Furthermore, several large animal models with features of metabolic syndrome have demonstrated alterations in coronary vascular function and structure.…”

Section: Animal Models Of Hfpefmentioning

confidence: 99%

Animal models of heart failure with preserved ejection fraction

et al. 2016

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Heart failure with preserved ejection fraction (HFpEF) constitutes a clinical syndrome in which the diagnostic criteria of heart failure are not accompanied by gross disturbances of systolic function, as assessed by ejection fraction. In turn, under most circumstances, diastolic function is impaired. Although it now represents over 50 % of all patients with heart failure, the mechanisms of HFpEF remain understood, precluding effective therapy. Understanding the pathophysiology of HFpEF has been restricted by both limited access to human myocardial biopsies and by the lack of animal models that fully mimic human pathology. Animal models are valuable research tools to clarify subcellular and molecular mechanisms under conditions where the comorbidities and other confounding factors can be precisely controlled. Although most of the heart failure animal models currently available represent heart failure with reduced ejection fraction, several HFpEF animal models have been proposed. However, few of these fulfil all the features present in human disease. In this review we will provide an overview of the currently available models to study HFpEF from rodents to large animals as well as present advantages and disadvantages of these models.

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Section: Animal Models Of Hfpefmentioning

confidence: 99%

Animal models of heart failure with preserved ejection fraction

et al. 2016

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“…The parameters to analyse these stages of DD include mitral inflow, pulmonary venous flow and valsalva maneuver. Of all, mitral E/A is widely used as marker for diastolic filling, which represents early to late filling velocities [18][19][20].…”

Section: Diastolic Dysfunctionmentioning

confidence: 99%

Diabetic Cardiomyopathy: A New Perspective of Mechanistic Approach

Muralidaran¹,

Viswanathan²

2015

J Diabetes Metab

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Diabetic cardiomyopathy (DCM), one among the macrovascular complications of diabetes is the leading cause of mortality around the globe. The emergence of DCM irrespective of other cardiovascular diseases (CVD) necessitates a deeper insight into its occurrence and mechanism of development. This review summarizes the different stages in DCM progression based on molecular, cellular and functional level. The early stage in DCM involves oxidative stress and endoplasmic reticulum stress concomitant with O-linked glycosylation of receptors, and extracellular matrix (ECM) proteins, and formation of advanced glycation end products (AGE). This further influences myocyte apoptosis and steatosis, which is followed by fibrosis and remodeling, thus altering the physiological functioning of heart. It has been postulated that, microbuminuria (MA) which is an indicator of renal failure is also an early predictor of DCM. Currently, focus on epigenetic modifications, as observed in diabetics with renal failure is of critical importance to unveil the mystery behind the association of DCM and MA. Albeit, we examine DCM in a mechanistic approach, the major reason that remains unnoticed is glycemic excursion (GE). ACCORD, DCCT and EDIC studies have reported that the persistent effect in CVD is due to transient hyperglycemia. Hence it is predicted that, treatment strategies focusing on genetic level, along with maintaining persistent blood glucose would be more effectual. Besides, designing treatment regimen for patients with renal failure would be a preventive strategy for diabetic cardiomyopathy when their state towards DCM incidence is also considered and monitored precisely.

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“…Magnetic resonance imaging (MRI) is currently one of the main imaging techniques for the assessment of cardiac function and morphology in human. Recently, cardiovascular MRI has also been successfully used in macaques [12][13][14]. For instance, cardiac effects of spontaneous type 2 diabetes [13] and the effects of myocardial infarction [14] on the cardiac performance have been studied in adult cynomolgus and rhesus macaques.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, cardiovascular MRI has also been successfully used in macaques [12][13][14]. For instance, cardiac effects of spontaneous type 2 diabetes [13] and the effects of myocardial infarction [14] on the cardiac performance have been studied in adult cynomolgus and rhesus macaques. At present, however, there is a lack of MR imaging protocols and reference values of cardiovascular MRI in marmosets.…”

Section: Introductionmentioning

confidence: 99%

Cardiac MRI in common marmosets revealing age-dependency of cardiac function

Moussavi

Mietsch

Drummer

et al. 2020

Preprint

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The aim of this study was to establish a feasible and robust magnetic resonance imaging protocol for the quantitative assessment of cardiac function in marmosets and to present normal values of cardiac function across different ages from young adult, middle-aged, to very old clinically healthy animals.Cardiac MRI of 33 anesthetized marmosets at the age of 2-15 years was performed at 9.4 T using IntraGate-FLASH that operates without any ECG-triggering and breath holding. Normalized to post-mortem heart weight, the left ventricular end-diastolic volume (LV-EDV) was significantly reduced in older marmosets. The LV end-systolic volume (LV-ESV) and the LV stroke volume (LV-SV) showed a similar trend while the LV ejection fraction (LV-EF) and wall thickening remained unchanged. Similar observations were made for the right ventricle. Moreover, the total ventricular myocardial volume was lower in older monkeys while no significant difference in heart weight was found.In conclusion, IntraGate-FLASH allowed for quantification of left ventricular cardiac function but seems to underestimate the volumes of the right ventricle. Although less strong and without significant sex differences, the observed age related changes were similar to previously reported findings in humans supporting marmosets as a model system for age related cardiovascular human diseases.Changes of the signal intensity related to respiratory movement could be detected on all navigator signal curves. Due to the respiratory frequency given by the artificial ventilation frequency analyses revealed always a sharp peak around 35 bpm. In addition, in most of the cases harmonics of the respiration were visible at multiples of 35 bpm. Figure 2 exemplarily shows diastolic and systolic images of two animals (Animals F and G) with good image quality throughout all acquired slices. In particular, papillary muscles and trabecularization were well resolved. Although residual flow artifacts and fuzziness may still be visible on these images, the achieved quality was sufficient for quantitative analysis of cardiac function. Cardiac Mass and FunctionAll marmosets were grouped by age into three groups for further analyses (young adult (< 5 years), middle-aged (5 -9 years), and senescent (>9 years). The respective group compositions as well as mean values of heart rate, body weight, and post-mortem heart weight are shown in Table 1. Marmosets, in contrast to most other primates, exhibit no somatic sex-dimorphism. Moreover, a two-way ANOVA (with age group and sex as independent variable) revealed no significant differences between the sexes in none of the analyzed parameters. For these reasons cardiac parameters obtained from male and female marmosets where pooled together (Table 2). Cardiac MassThe weight of the hearts, measured post-mortem and including ventricles and atria, ranged from 1.85 g to 3.13 g (2.6 ± 0.4 g, mean ± standard deviation). This weight was positively correlated with the body weight of the animal (Pearson's r = 0.54, p < 0.01) and the ventricle myocardia...

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Diastolic dysfunction in spontaneous type 2 diabetes rhesus monkeys: a study using echocardiography and magnetic resonance imaging

Cited by 27 publications

References 54 publications

Animal models of heart failure with preserved ejection fraction

Animal models of heart failure with preserved ejection fraction

Diabetic Cardiomyopathy: A New Perspective of Mechanistic Approach

Cardiac MRI in common marmosets revealing age-dependency of cardiac function

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