Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)

Abstract

Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya , previously the Pectobacterium chrysanthemi complex ( Erwinia chrysanthemi ), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi , showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya . The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii , these isolates represent a novel species within the genus Dickeya , for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.

Dickeya fangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (Pyrus pyrifolia)

“…Subsequent revision of this group led to the further reclassification of D. dieffenbachiae as a subspecies of D. dadantii (Brady et al, 2012). Recently, isolates from European potatoes were classified in the genus Dickeya (Slawiak et al, 2009), and were considered to represent a novel species, Dickeya solani (van der Wolf et al, 2014). Furthermore, three isolates (174/2 T , 181/ 2 and Dw054) from different waterways in the UK and Finland have been classified as representatives of a novel species, Dickeya aquatica .…”

“…Plates were inoculated according to the manufacturer's recommendations and incubated at 28 C. The biochemical tests (Table 1) used were as previously selected for differential identification of Dickeya species (Samson et al, 2005;Slawiak et al, 2009;van der Wolf et al, 2014;Parkinson et al, 2014). Biochemical reactions were carried out for all three strains (JS5 T , LN1 and QZH3).…”

A review on bacterial stalk rot disease of maize caused by Dickeya zeae

“…The nucleotide sequence of the genomes of several phytopathogenic bacteria, such as Agrobacterium tumifaciens, Pseudomonas syringae, Ralstonia solanacearum, Xylella fastidiosa and two Xanthomonas oryzae and many species of soft rot Erwinia recently determined (Simpson et al, 2000;Buell et al, 2003;Lee et al, 2005;Salanoubat et al, 2002;Wood et al, 2001;Pritchard et al (2013). The Dickeya genus is recentely described six species: dianthicola, dadantii, zeae, chrysanthemi, paradisiacal and solani (Samson et al, 2005;Brady et al, 2012;Van der et al, 2013). Draft genome sequences of eight D. dianthicola and D. solani isolates were recently described , and four complete sequences of Dickeya strains, D. paradisiaca (Ech703), D. zeae (Ech586), D. chrysanthemi (Ech1591) and D. dadantii (Ech3937) have been deposited in GenBank (Glasner et al (2011).…”

Biocontrol of the Potato Blackleg and Soft Rot Diseases Caused by Dickeya dianthicola

“…In Europe, the pectinolytic pathogens on potato crops include Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, Dickeya dianthicola, and Dickeya solani (4)(5)(6)(7)(8). Recently, new Pectobacterium taxa, i.e., P. wasabiae and P. carotovorum subsp.…”