Die Amitose der Tierischen und Menschlichen Zelle

Bucher, Otto

doi:10.1007/978-3-7091-5467-0

Cited by 18 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Levi (1934) in his review of the Lewis's work did not agree with their conclusions but seemed to prefer the interpretation that amitosis produced multinucleation. Bucher (1959) in his excellent review of evidence for and against the occurrence of amitosis concluded that this method of nuclear division is used in many cell types including chick skeletal muscle. He cautiously adds, however, that critical evidence is lacking in most cases.…”

Section: Introductionmentioning

confidence: 99%

Multinucleation of Skeletal Muscle in vitro

Capers

1960

The Journal of Cell Biology

164

View full text Add to dashboard Cite

Healthy, mature, spontaneously contracting muscle was cultivated from explants of 13-day chick embryos for periods up to 4 months in the multipurpose chamber (Rose, 1954) using cellophane-strlp technique (Rose eta/., 1958) with silicone gaskets, Eagle's medium including 10 per cent horse serum reinforced with 300 mg-per cent of glucose, and the teased type of explant. This method provided optically ideal conditions for the study of muscle fibers with oil immersion, phase contrast time-lapse cinematography at 1 frame per minute without apparent damage for periods as long as 10 days.In no case was mitosis, amitosis, or nuclear "budding" observed in the course of muscle development. Multinuclear muscle fibers have been shown with cine technique to result from both myoblast fusion and polar extension of preformed (explanted) muscle tissue. Myoblast fusion was the only demonstrable way of giving rise to multinucleation.Nuclear membrane "wrinkling" was shown to be merely a temporary distortion that occurred during nuclear migration and rotation. It is suggested that this phenomenon may be responsible for numerous reports of amitosis in the genesis of muscle fibers.The histological development of new straps resulted from an orderly sequence of events. Included in these were polar extension, nuclear migration, rotation, and fixation. Following these events there was increased mitochondrial activity, myofibril formation, and cross-banding. Spontaneous contractions were seen throughout the entire course of differentiation in vitro but became more regular and stronger in the later stages.

show abstract

Section: Introductionmentioning

confidence: 99%

Multinucleation of Skeletal Muscle in vitro

Capers

1960

The Journal of Cell Biology

164

View full text Add to dashboard Cite

show abstract

“…We are now able to combine our observations on progenitor cells thus varifying branch B of the model, but adding the open endomitosis (D) and the nuclear amitosis (E). Amitosis may be proved only in observations of liv ing cells [13]. Probably chromosome dou bling takes place before nuclear amitosis, if nuclear growth occurs but this cannot be confirmed in living cell preparations.…”

Section: Resultsmentioning

confidence: 99%

“…The nuclear lobes may facili tate the exchange of necessary materials in these massively nucleated cells [2,13].…”

Section: Resultsmentioning

confidence: 99%

Origin of the Early Megakaryopoietic Progenitor Cell and Further Polyploidization in the Thrombopoietic Cell Series. Phase-Contrast Observations of Human Bone Marrow

Boll¹

1981

Acta Haematol

View full text Add to dashboard Cite

Continuous observation of living cells by phase-contrast time lapse microcinematography revealed different origins of multinuclear cells and cells with lobed nuclei in the hematopoietic progenitor cell series, which must be classified as megakaryoblasts, the determinated progenitor cells of the megakaryopoietic series. Hematopoietic progenitor cells with single nuclei of nuclear diameter 5–13 μm, the promegakaryoblasts, may become polyploid by various means: by postmitotic cytoplasmic fusion, by consecutive nuclear fusion, by endomitosis, or by nuclear amitosis or lobe formation presumably after endoreduplication and nuclear growth. Including amitotic nuclear division in the mitotic rate of normal progenitor cells nearly the half of all these cells in G₂ phase (0.4) produce in vitro octoploid megakaryoblasts, respectively osteoclasts. Thrombopoietin-rich sera in the medium enhance the mitotic index of the hematopoietic progenitor cell line from 0.1 to 0.25, but do not change the proportion of polyploid mitoses.

show abstract

“…Кроме того, появление гантелевидных пере тяжек ядра также относили к морфологическим признакам амитоза -прямому способу деления клеток, при котором компоненты ядра распреде ляются между дочерними ядрами неравномерно [19,35]. Однако позднее было продемонстри ровано, что гантелевидные ядра могут форми роваться в интенсивно делящихся клетках при низкой температуре окружающей среды и в усло виях недостатка питательных веществ [13]. Таким образом, механизм возникновения такой формы ядра находится под вопросом.…”

Section: Cytological Anomalies After Ionizing Radiation Exposureunclassified

“…In addition, the appearance of dumbbelllike nuclei was also attributed to the morphologi cal features of amitosis, the direct method of cell division, in which the components of the nucleus are distributed unevenly between the daughter nuclei [19,35]. However, later it was demonstrated that dumbbell nuclei can be formed in intensively dividing cells at low temperature and in conditions of nutrients starvation [13]. Thus, the mechanism for the appearance of such a form of the nucleus is not clear.…”

Section: Cytological Anomalies After Ionizing Radiation Exposurementioning

confidence: 99%