Die Wirkung von Actidion auf die Synthese des Herpes-simplex-Virus in Kulturen verschiedener Zellarten

Falke, D.; Kohlhage, H.; Niessing, Klaus

doi:10.1515/znb-1966-0512

Cited by 11 publications

(4 citation statements)

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“…FFWI com mences 3-4 h after infection in both cell types, whereas FFWO is complete after 45-60 min. In addition, FFWI induced by strains JES and HFF.M is detectable at 4 h postinfection, as shown previously for strain JES [15].…”

Section: Discussionsupporting

confidence: 86%

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Fusion from Without Induced by Herpes Simplex Virus Type 1

Falke

Knoblich

Müller³

1985

Intervirology

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Strains ANG and ANG path of herpes simplex virus type 1 (HSV1) produced fusion from without (FFWO) of cells in culture. FFWO required 45min to become complete. In contrast, fusion from within (FFWI) was not detected until 3–4 h after infection, depending on the cell type. FFWO was temperature dependent: at 0° no fusion could be observed, but increase of temperature increased the degree of fusion. The pH optimum for FFWO was 7.8–8.5. The FFWO activity of the virus was found to be slightly more heat stable at 46° than was infectivity. FFWO was produced in Vero, CV-1 and BSCl cells, but not in BHK clone 13 or in primary or secondary rabbit kidney cells. FFWO was linked to the presence of virus particles and perhaps to other sedimentable, infected-cell material but not to soluble factors. Actinomycin D, cycloheximide, and UV irradiation did not block this activity, indicating no direct activity of the HSV1 genome for FFWO.

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Section: Discussionsupporting

confidence: 86%

“…AM and CH did not inhibit FFWO produced by strain ANG. In contrast, FFWI (produced comparably by strain JES in Vero cells) was susceptible to the action of AM and CH as shown earlier [15,16]. Cpd 48/80 only partially inhibited FFWO, in con trast to its selective and complete blockade of FFWI [5].…”

Section: Ffwo Produced By Hsvisupporting

confidence: 62%

Fusion from Without Induced by Herpes Simplex Virus Type 1

Falke

Knoblich

Müller³

1985

Intervirology

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“…To obtain solitary localized giant cells, Leighton tubes were infected with about 200 P F U (low MOI). U~der standard conditions with high MOI, all cells of a Leighton tube developed into large polycaryocytes at 7--8 hours after infection (7). Titrations were done as described earlier (8).…”

Section: Cellsmentioning

confidence: 99%

Microtubules and microfilaments in HSV-infected rabbit-kidney cells

Heeg

Haase

Brauer

et al. 1981

Archives of Virology

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In rabbit kidney cells infected with strains of Herpes simplex virus producing either cell-rounding or polycaryocytosis. Vinblastine induced paracrystals. This could be shown by phase-contrast- and electron-microscopy. Infections were done under one-step-growth conditions or at low MOI. 90 per cent noninfected cells contained stress fibers as detected by Servablue R250-staining. Shortly after recruitment into polycaryocytes, stress fibres of normal length appearing in criss-cross arrangement can be seen in the periphery of these cells. Later they polymerize to very long fibers and finally they are partially destroyed. The time of destruction depends on the MOI employed. By using Actinomycin D and/or Cycloheximide as blocking agents, it could be shown that polymerization of microfilaments correlates in time with giant cell formation. In view of the fact that the virus synthesis is accompanied in parallel by a special rearrangement of microfilaments as well as polycaryocytosis, both these processes have to be considered as caused by early (and late ?) protein-synthesis (beta-/gamma-proteins) but not as induced by "very-early" proteins (alpha-proteins).

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“…Die Angaben iiber die Zellziichtung, die Kulturmedien und die Virussti~mme sind in friiheren Ver6ffentlichungen niedergelegt (8,11 Immunadharenz. Die IAR und IAH wurde wie besehrieben durchgefiihrt (9,10).…”

Section: Materials Und Methoden Aunclassified