Dieckol, isolated from Ecklonia stolonifera, induces apoptosis in human hepatocellular carcinoma Hep3B cells

Abstract

Phlorotannins have been reported to demonstrate several biological properties, including antioxidant activity, and activities useful in the treatment of diabetic complications and in chemoprevention of several vascular diseases. In this study, we focused on the apoptosis induced by dieckol, a marine algal phlorotannin isolated from Ecklonia stolonifera, on human hepatocellular carcinoma (HCC) Hep3B cells. Dieckol reduced the numbers of viable cells and increased the numbers of apoptotic cells in a dose-dependent manner. Immunoblotting analysis revealed that dieckol increased the expression levels of cleaved caspases-3, 7, 8, and 9, and cleaved poly(ADP-ribose) polymerase. Dieckol increased the permeability of mitochondrial membranes and the release of cytochrome c from mitochondria into the cytosol with apoptosis-inducing factor. In addition, dieckol induced increased expression of truncated Bid and Bim. The results indicate that dieckol induces apoptosis via the activation of both death receptor and mitochondrial-dependent pathways in HCC Hep3B cells.

Involvement of NF-ΚB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens

“…In addition, it also performs a crucial role in the induction of cell death initiated by a variety of stimuli 64. The proteolytic degradation of PARP that takes place at the beginning of apoptosis is caused by the activated caspase-3 65. As demonstrated by the immunoblot analysis, AM treatment significantly increased thee cleaved PARP protein.…”

Brown Algae <i>Himantothallus grandifolius</i> (Desmarestiales, Phaeophyceae) Suppresses Proliferation and Promotes Apoptosis-Mediated Cell Death in Tumor Cells

“…has shown cytocompatible effects to human fetal lung fibroblasts cell line (MRC-5) [28] and macrophage-like form cell (THP-1 cell) [15]. A recent report using dieckol, an isolated compound from Ecklonia stolonifera Okamura algae extracts, also presented more selective effects to Hek-293 compared to a tumor cell line Hep3B [29].…”

Dieckol, a major phlorotannin in Ecklonia cava, suppresses lipid accumulation in the adipocytes of high‐fat diet‐fed zebrafish and mice: Inhibition of early adipogenesis via cell‐cycle arrest and AMPKα activation

“…Dieckol is known to be a major phlorotannin of both Seapolynoland Ecklonia cava itself . Various biological studies of dieckol to date have been reported . Lee et al.…”