Biodiesel plays an important role in reducing the dependency of petroleum fuels and reduce environmental pollution. Biodiesel has attracted attention as a renewable, non-toxic, and biodegradable fuel. In the past years, researchers have expanded their work to new methods of obtaining biodiesel. In this work, biodiesel production using soybean oil from the industry is described. Biodiesel was further analyzed and compared with the EN biodiesel specifications. The characterization of biodiesel was performed in order to obtain density, viscosity and flash point. Moreover, the study was focused in optimized the biodiesel yield, obtain from soybean oil using Artificial Neural Networks (ANN). The variable parameters were molar ratio between methanol and oil, reaction temperature and catalyst quantity. The paper concludes that the ANN can be successfully used to optimize the biodiesel yield.