Dietary avocado oil prevents the impairment in the complex III and ROS production in kidney mitochondria from diabetic rats by improving the electron flow through cytochrome c1

Abstract

ROS production at mitochondrial complex III (CIII) has been identified as one of the main factors leading to diabetic nephropathy. ROS production at CIII during diabetes occurs due to damage in some of its subunits, being oxidative damage a main contributor for this phenomenon. Thus, the attenuation of the damage in the CIII may serve as an approach to ameliorate diabetic renal dysfunction. Because its antioxidant content, we aimed to investigate whether avocado oil (AO) could decrease both the impairment in CIII and ROS production in kidney mitochondria from STZ‐induced diabetic rats. 90‐days of dietary AO administration prevented the impairment in CIII activity and decreased ROS production when the complex was further damaged by Fe‐induced oxidative stress. Impairment in electron flow through cytochrome c1 subunit was identified as the cause of CIII impairment and avocado oil also prevented this effect. Together, these results suggest that dietary avocado oil decrease the damage in the CIII by avoiding haem c1 destruction, which may be related with the high content of C18:1 and antioxidants in AO. This work was funded by CONACYT (130638 to CCR), and CIC‐UMSNH (CCR) grants.