Dietary Emulsifiers Alter Composition and Activity of the Human Gut Microbiota in vitro, Irrespective of Chemical or Natural Emulsifier Origin

Miclotte, Lisa; Paepe, Kim De; Rymenans, Leen; Callewaert, Chris; Raes, Jeroen; Rajković, Andreja; Camp, John Van; Wiele, Tom Van de

doi:10.3389/fmicb.2020.577474

Cited by 43 publications

(48 citation statements)

References 121 publications

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“…Additives such as: -Dietary emulsifiers: sodium CMC, P80, soy lecithin, sophorolipids, and rhamnolipids. [34,50,[54][55][56][57][58][59] To assay the gut microbiota metabolism in response to food components.…”

Section: Main Objective Study Subject Referencesmentioning

confidence: 99%

“…After the collection, an individual faecal sample or faecal pool is mixed with a solution (e.g., phosphate buffered saline or sodium chloride) to obtain a faecal suspension or faecal slurry (e.g., 10% w/v or 20% w/v). Then, the suspension is manually homogenised or is homogenised using a stomacher [31,36,43,56,69]. In addition to the previous homogenisation stage, some protocols include filtering [27,30,54], or centrifugation steps [37,65,80] so that the supernatant is used as faecal inoculum in the fermentation process.…”

Section: Faecal Inoculum Collection and Preparationmentioning

confidence: 99%

“…In parallel, other methodologies can be used to target or quantify specific bacteria genera or species or total bacteria, depending on the specificity of the primer used (e.g., qPCR, PCR-DGGE gel or FISH) [26,53,80,102]. When it comes to metabolite analysis, SCFA or BCFA production are typically analysed using gas or liquid chromatography (GC or LC) coupled with either different detectors (e.g., FID or UV) or MS [43,54,56], but when other metabolites, such as phenolic compounds, are being considered in the studies, LC techniques coupled with MS [44,102] or antioxidant tests are commonly used when studying antioxidant capacity [33]. Finally, ammonium, pH, lactic acid, and gas volume measurements are also analysed in most of the studies [36,65,71].…”

Section: Changes In the Microbiota Populations And Their Metabolic Responsementioning

confidence: 99%

“…[ 56,75] To assay sample processing (extrusion, and diverse thermal treatments) on gut microbiota -Gut microbiota contents: 16S rDNA Sequencing, qPCR, or FISH.…”

Section: Main Objective Analytical Determinations Referencesmentioning

confidence: 99%

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In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

et al. 2021

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The human colonic microbiota plays an important role in the food digestion process and has a key role in maintaining health status. This community of microbes is inter-individually different due to several factors that modulate its composition. Among them, diet is one of the most relevant, which, in turn, is affected by environmental, economic, and cultural considerations. These pieces of evidence have promoted the study of the influence of diet on gut microbiota and the development of in vitro models that simulate the colonic digestion of foods. This narrative review aims to present a technical approach of the in vitro gut models available to evaluate the impact of diet on human colonic microbiota. A description and comments on the main characteristics, parameters, applicability, faecal inoculum preparation, and analytical tools are made. Despite the progress of in vitro colonic digestion models and metaomic applicability in this research field, there are still some challenges to face due to the lack of a consensus on the methodologies to conduct in vitro colonic digestions and the need to integrate the metaomic data to fully understand the influence of food in human colonic microbiota.

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Section: Main Objective Study Subject Referencesmentioning

confidence: 99%

Section: Faecal Inoculum Collection and Preparationmentioning

confidence: 99%

Section: Changes In the Microbiota Populations And Their Metabolic Responsementioning

confidence: 99%

“…[ 56,75] To assay sample processing (extrusion, and diverse thermal treatments) on gut microbiota -Gut microbiota contents: 16S rDNA Sequencing, qPCR, or FISH.…”

Section: Main Objective Analytical Determinations Referencesmentioning

confidence: 99%

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In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

et al. 2021

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“…henodeoksiholna i deoksiholna kiselina), nastale crijevnom mikrobiotom posredovanom dehidroksilacijom žučnih kiselina (gubitak 7a-hidro-droksilna grupa na jezgri žučne soli), povećavaju unos bakterija u debelom crijevu čoveka. 37 Značajno povećanje bakterijske dezoksiribonukleinske kiseline u krvi verifikovano je kod oboljelih od kardiovaskularnih bolesti, dijabetesa tipa 2 i Kronove bolesti. 37 Koncentracija bakterijske dezoksiribonukleinske kiseline u krvi oboljelih od Kronove bolesti je važan prognostički faktor kasnijih recidiva.…”

Section: Toksikološki Efekti Prehrambenih Aditivaunclassified

Real and perceived risk: Food additives

Jandrić-Kočić¹

2021

Med gl Spec bol štit Zlatibor

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Food additive is any substance of known chemical composition that is not normally used as food in itself or is a typical food ingredient, regardless of nutritional value, and is added specifically for technological and organoleptic properties of food in the technological process of production, during preparation, processing, finishing, processing, shaping, packaging, transport and storage, which leads or can be expected to lead to the fact that he or his by-product directly or indirectly becomes an ingredient of that food. Food additives are a necessity in food production technology. Their use causes consumer distrust and controversy among the professional public. The negative attitude of a significant number of consumers due to the lack of awareness of the legislation that precedes the approval of food additives can be removed through open, transparent, independent, responsible / timely communication on risk. On the other hand, research on inadequate labeling, toxicity and the use of illicit food additives requires continuous monitoring, improvement of the control system and elimination of deficiencies. The safe use of food additives is a joint responsibility of the state, producers, distributors, the profession, ie laboratories, but also the consumers themselves.

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A comprehensive review on pleiotropic effects and therapeutic potential of soy lecithin

Mangrulkar,

Kulkarni,

Nanepag

et al. 2024

ADV TRADIT MED (ADTM)

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Dietary Emulsifiers Alter Composition and Activity of the Human Gut Microbiota in vitro, Irrespective of Chemical or Natural Emulsifier Origin

Cited by 43 publications

References 121 publications

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

Real and perceived risk: Food additives

A comprehensive review on pleiotropic effects and therapeutic potential of soy lecithin

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