Dietary fish oil up‐regulates cholesterol 7α‐hydroxylase mRNA in mouse liver leading to an increase in bile acid and cholesterol excretion

Bérard, Annie M.; Dumon, M.F.; Darmon, Michel

doi:10.1016/s0014-5793(04)00049-3

Cited by 38 publications

(33 citation statements)

References 28 publications

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“…CYP7A1 is induced by PPAR/RXR or LXR/RXR, but is inhibited by PPAR/LXR 39) . Fish oil stimulates RXR alpha expression markedly more than LXR 36) . This observation suggests that EPA induces RXR mRNA more than DHA and that the marked increase of CYP7A1 mRNA expression in the MO diet with fenofibrate group in this study was induced by formation of a PPAR/RXR or LXR/RXR combination from the induction of RXR and PPAR by EPA and fenofibrate, respectively.…”

Section: Discussionmentioning

confidence: 93%

“…The data presented here indicate that the increment of the plasma total cholesterol level in fenofibrate-supplemented groups is due to the increase of the plasma HDL-cholesterol level ( Table 2). Fish oil up-regulates CYP7A1 as well as increasing bile acid and cholesterol excretion 36) . Furthermore, fenofibrate induces CYP7A1 expression through PPAR 37,38) .…”

Section: Discussionmentioning

confidence: 99%

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Interaction of Fenofibrate and Fish Oil in Relation to Lipid Metabolism in Mice

Arai

Kim

Chiba

et al. 2009

JAT

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Aim:The aim of our study is to elucidate the interactive effects on lipid metabolism of fenofibrate and two fish oils with EPA and DHA contents in mice. Methods: Female C57BL/6J mice were fed purified experimental diets containing safflower oil (SO), EPA-rich menhaden oil (MO) or DHA-rich tuna oil (TO) with or without 0.1% fenofibrate for 8 weeks. At the end of the experiments, we measured plasma lipids and hepatic triglycerides and cholesterol, and the hepatic mRNA expression of lipogenic and lipidolytic genes. Results: Plasma TG levels fell in the group fed MO or TO alone and fell significantly in all fenofibrate-treated groups. Although plasma total cholesterol levels fell significantly in fish oil-fed groups, fenofibrate treatments increased significantly plasma total cholesterol levels in these fish oil groups, but not in the group fed SO alone; however, hepatic triglyceride and total cholesterol levels markedly decreased in MO-or TO-fed mice. In lipid synthesis, the hepatic mRNA level of SREBP-1c was not reduced in either fish oil group; however, Insig-1 mRNA decreased in MO and TO feeding groups by about half and FAS or SCD-1 mRNA decreased significantly in MO and TO feeding groups, compared with the SO feeding group. In both fish oil groups, SREBP-2 mRNA decreased significantly and HMG-CoA reductase mRNA also decreased with/without fenofibrate. On the other hand, fenofibrate supplementation significantly induced the mRNA expression of AOX and UCP-2, which play a role in lipid catabolism, in all diets. CYP7A1 mRNA increased markedly in mice fed MO diet with fenofibrate, compared with TO diet with fenofibrate. Conclusion: These data suggest that differences in dietary contents of EPA and DHA do not influence the inhibition of lipogenesis, and that fenofibrate supplementation stimulates fatty acid oxidation, regardless of the oil type; however, cholesterol catabolism was induced by a combination of EPA-rich fish oil and fenofibrate, which suggests that EPA has a greater synergistic ability for cholesterol catabolism induction by fenofibrate than DHA. J Atheroscler

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Interaction of Fenofibrate and Fish Oil in Relation to Lipid Metabolism in Mice

Arai

Kim

Chiba

et al. 2009

JAT

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“…The results suggest that 4.5% fish oil in the butter blend does not play an important role in acute cholesterol absorption. It has however been shown that n-3 PUFA via effects on transcription factors can upregulate 7a-hydroxylase mRNA in mouse liver leading to an increase in bile acid and cholesterol excretion [35]. Therefore it cannot be rule out that a long-term consumption of the butter blend containing fish oil may affect the cholesterol levels in plasma.…”

Section: Discussionmentioning

confidence: 99%

Postprandial lipid responses of butter blend containing fish oil in a single‐meal study in humans

Overgaard

Porsgaard

Guo

et al. 2008

Molecular Nutrition Food Res

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The postprandial effects of a butter product containing fish oil were investigated in a single-meal, randomized crossover study with a commercial butter product as the control. Twelve healthy males consumed two test meals with (13)C-labelled cholesterol (45 mg) and either an interesterified butter blend with fish oil (352 mg n-3 long-chain PUFA (LCPUFA)) or the commercial butter blend. Blood samples were collected after the meals and in the fasting condition on the test day and the following morning, and were analysed for cholesterol absorption, plasma lipid profile and fatty acid composition. No significant difference in the postprandial plasma fatty acid composition was observed between the groups, neither difference in cholesterol absorption, plasma cholesterol or the cholesterol contents of plasma lipoproteins. The incorporation of fish oil in the butter resulted in a significant lower concentration of triacylglycerols in the plasma 2 h after the meal in comparison with the commercial butter blend (p = 0.02); there was, however, no significant difference 24 h after the meal. In conclusion, fish oil-enriched butter blend provides a source to increase the intake of n-3 LCPUFA in the population, but has no acute effect on cholesterol absorption and plasma cholesterol concentration in human.

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“…The fecal excretion of bile acids in the CϩEX and DF/KBRϩEX groups was significantly higher than that in the C group. It has been reported that dietary fish oil increases the level of hepatic CYP7A1 mRNA in mice (35) and that the enzyme activity of CYP7A1 correlates with the increase in the mRNA level (36). Fish oil enhances bile acid synthesis in rats (37) suggesting that bile acid synthesis would be increased in the EX and DF/KBRϩEX groups in comparison to the C group.…”

Section: Discussionmentioning

confidence: 99%