Dietary manganese source does not affect Mn, Zn and Cu tissue deposition and the activity of manganese-containing enzymes in lambs

Grešaková, Ľubomíra; Venglovská, Katarína; Čobanová, Klaudia

doi:10.1016/j.jtemb.2016.05.003

Cited by 26 publications

(17 citation statements)

References 27 publications

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“…The mineral content in the diet substrates and the serum concentrations of zinc, iron and copper in the lambs were determined by flame atomic absorption spectrometry in an air-acetylene flame, with deuterium background correction [ 26 ], using an AA-7000 atomic absorption spectrophotometer (Shimadzu Co., Kyoto, Japan). Certified lyophilized human plasma, ClinCheck Control (Recipe, Munich, Germany), was used to determine the precision of the analysis.…”

Section: Methodsmentioning

confidence: 99%

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

et al. 2018

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BackgroundThe gastrointestinal parasitic nematode Haemonchus contortus is a pathogenic organism resistant to several anthelmintics. This study assessed the efficacy of a medicinal herbal mixture (Herbmix) and organic zinc, as an essential trace element for the proper functioning of both unspecific and specific immune defensive mechanisms, against experimental infections with H. contortus in lambs. All lambs were infected orally with approximately 5000 third-stage larvae of a strain of H. contortus susceptible to anthelmintics (MHco1). Twenty-four female lambs 3–4 months of age were divided into four groups: unsupplemented animals (control), animals supplemented with Herbmix (Hmix), animals supplemented with organic zinc (Zn) and animals supplemented with Herbmix and organic zinc (Hmix+Zn). Eggs per gram (EPG) of faeces were quantified 20, 28, 35, 42, 49, 56, 62 and 70 d post-infection and mean abomasal worm counts were assessed 70 d post-infection. Samples of blood were collected from each animal 7, 35, 49 and 70 d post-infection.ResultsQuantitative analyses of the bioactive compounds in Herbmix identified three main groups: flavonoids (9964.7 μg/g), diterpenes (4886.1 μg/g) and phenolic acids (3549.2 μg/g). Egg counts in the lambs treated with Hmix, Zn and Hmix+Zn decreased after 49 d. The EPGs in the Zn and Hmix+Zn groups were significantly lower on day 56 (P < 0.05 and P < 0.01, respectively), and the EPGs and mean worm counts were significantly lower on day 70 in all supplemented groups (P < 0.05 and P < 0.01). Hemograms of complete red blood cells of each animal identified clinical signs of haemonchosis after day 35. Serum calprotectin concentrations and IgA levels were significantly affected by treatment. The treatment influenced serum malondialdehyde concentrations (P < 0.05) and sulfhydryl groups (P < 0.01) of antioxidant status. The mineral status was unaltered in all lambs.ConclusionA direct anthelmintic impact on the viability of nematodes was not fully demonstrated, but the treatments with herbal nutraceuticals and zinc likely indirectly contributed to the increase in the resistance of the lambs to nematode infection.

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Section: Methodsmentioning

confidence: 99%

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

et al. 2018

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“…All samples except the plasma were dried (105°C for 48 h) and ground for subsequent wet digestion with a concentrated nitric acid and hydrogen peroxide mixture (3:1) in a microwave digestion system (MWS 4, Berghof Co., Germany). The plasma samples were only diluted (1:10) with 0.05% Triton X-100 solution (Gresakova et al, 2016). The concentration of Mn in plasma as well as the Mn and Cu content in The vitamin/mineral premix provided per kg of complete diet: Vitamin A 6000 IU; Vitamin D3 1000 IU; Vitamin E, 50 mg; Vitamin B1, 1.7 mg; Vitamin B2, 8.0 mg; Vitamin B6, 3.0 mg; Vitamin B12, 0.01 mg; Vitamin K3, 0.5 mg; biotin, 0.2 mg; folic acid, 0.5 mg; nicotinic acid, 70 mg; choline chloride, 700 mg; Mn, 50 mg; Fe, 40 mg; Cu, 30 mg; Se, 0.2 mg. c Metabolisable energy was calculated by the equation of Wiseman et al (1992).…”

Section: Chemical Analysis and Measurementsmentioning

confidence: 99%

The effect of different dietary zinc sources on mineral deposition and antioxidant indices in rabbit tissues

et al. 2018

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The purpose of this study was to compare the effect of dietary zinc from inorganic and organic sources on the concentration of Zn, Cu, Mn and Fe in plasma, tissues and faeces of rabbits. Simultaneously, the activities of total superoxide dismutase (SOD), specific Cu/Zn SOD, glutathione peroxidase (GPx), lipid peroxidation and total antioxidant capacity (TAC) in liver and kidney were also determined. Ninety-six 49-day-old broiler rabbits were allocated to 4 dietary treatments, each replicated 6 times with 4 animals per replicate. For the subsequent 6 wk, the rabbits were fed an identical basal diet (BD) supplemented with an equivalent dose of Zn (100 mg/kg) from different sources. Group 1 (control) received the unsupplemented BD, while the BD for groups 2, 3 and 4 was supplemented with Zn from Zn sulphate, Zn chelate of glycine hydrate (Zn-Gly) and Zn chelate of protein hydrolysate (Zn-Pro), respectively. The intake of dietary Zn sulphate resulted in an increase in Zn plasma concentration (1.85 vs. 1.48 mg/L; P<0.05) compared to the control group. Feeding the diets enriched with Zn increased the deposition of Zn in the liver (P<0.05), irrespective of the Zn source. The addition of Zn-Pro resulted in significantly higher Cu uptake in liver (P<0.05) than in the control and Zn sulphate group (56.0 vs. 35.0 and 36.7 mg/kg dry matter (DM), respectively). Neither Mn nor Fe concentration in plasma and tissues were affected by dietary Zn supplementation, with the exception of Fe deposition in muscle, which was significantly decreased (P<0.05) in rabbits supplemented with inorganic Zn sulphate compared to control and Zn-Gly group (9.8 vs. 13.3 and 12.2 mg/kg DM, respectively). Intake of organic Zn-Gly significantly increased the activities of total SOD (43.9 vs. 35.9 U/mg protein; P<0.05) and Cu/Zn SOD (31.1 vs. 23.8 U/mg protein; P<0.01) as well as TAC (37.8 vs. 31.2 μmol/g protein; P<0.05) in the kidney when compared to that of the control group. The presented results did not indicate any differences between dietary Zn sources in Zn deposition and measured antioxidant indices in rabbit tissues. Higher dietary Zn intake did not cause any interactions with respect to Mn, Cu and Fe deposition in liver and kidney tissues, but did increase the faecal mineral concentrations. Dietary organic Zn-Gly improved the antioxidant status in rabbit kidney.

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“…The addition of these compounds at the permitted level does not threaten either the safety of consumers or the environment [ 5 ]. The organic forms of microelements are considered to be more available to animals [ 6 , 7 ]. Relative bioavailability of manganese from manganese methionine was 120% of that present in the sulfate form in experiments conducted with lambs [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem

et al. 2018

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Little is known about the effects of the high dose and types of manganese supplements on rumen environment at manganese intake level close above the limit of 150 mg/kg of dry feed matter. The effects of high dose of two manganese supplements (organic and inorganic) on rumen microbial ecosystem after four months of treatment of 18 lambs divided into three treatment groups were studied. We examined the enzyme activities (α-amylase, xylanase, and carboxymethyl cellulase), total and differential microscopic counts of rumen ciliates, total microscopic counts of bacteria, and fingerprinting pattern of the eubacterial and ciliates population analyzed by PCR-DGGE. Lambs were fed a basal diet with a basal Mn content (34.3 mg/kg dry matter; control) and supplemented either with inorganic manganous sulfate or organic Mn-chelate hydrate (daily 182.7, 184 mg/kg dry matter of feed, respectively). Basal diet, offered twice daily, consisted of ground barley and hay (268 and 732 g/kg dry matter per animal and day). The rumens of the lambs harbored ciliates of the genera of Entodinium, Epidinium, Diplodinium, Eudiplodinium, Dasytricha, and Isotricha. No significant differences between treatment groups were observed in the total ciliate number, the number of ciliates at the genus level, as well as the total number of bacteria. Organic Mn did decrease the species richness and diversity of the eubacterial population examined by PCR-DGGE. No effects of type of Mn supplement on the enzyme activities were observed. In comparison to the control, α-amylase specific activities were decreased and carboxymethyl-cellulase specific activities were increased by the Mn supplements. Xylanase activities were not influenced. In conclusion, our results suggested that the intake of tested inorganic and organic manganese supplements in excess may affect the specific groups of eubacteria. More studies on intake of Mn supplements at a level close to the limit can reveal if the changes in microbial population impact remarkably the other rumen enzymatic activities.

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Dietary manganese source does not affect Mn, Zn and Cu tissue deposition and the activity of manganese-containing enzymes in lambs

Cited by 26 publications

References 27 publications

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

The effect of different dietary zinc sources on mineral deposition and antioxidant indices in rabbit tissues

The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem

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