Dietary Plasma Protein Supplements Prevent the Release of Mucosal Proinflammatory Mediators in Intestinal Inflammation in Rats

Pérez-Bosque, Anna; Miró, Lluïsa; Polo, Javier; Russell, Louis; Campbell, Joy; Weaver, Eric; Crenshaw, J. D.; Moretó, Miquel

doi:10.3945/jn.109.112466

Cited by 62 publications

(78 citation statements)

References 32 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SEB stimulates lymphocytes to secrete interferon-g and TNFa (37) and this is correlated with reduced tight-junction protein expression (21) and increased permeability (28) . In our model, SEB administration also increased the release of TNFa and interferon-g, IL-6 and leukotriene B 4 in both mucosa and PP (38) . In addition, the stimulation of mucosal GALT by SEB led the mucosal immune response to a T-helper 1 response, since IFN-g and TNFa enhanced activation and proliferation of T-helper 1 cells (39) .…”

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 80%

“…In pigs challenged with E. coli K88, Bosi et al (49) observed that the expression of proinflammatory cytokines was lower in animals fed SDP. Results from our laboratory indicate that plasma supplements reduce the expression of mucosal pro-inflammatory cytokines (50,38) and the activation of T-helper subsets in the lamina propria and in the epithelium (32) . These effects on mucosal cytokine profile can explain the reduction of mucosal inflammation by plasma supplements and the preventive effects of SDP and IC on changes in the mucosal permeability (31) , tight-junctional protein expression (35) and nutrient absorption (36) following SEB administration.…”

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

“…Experimental groups were: Control group; rats administered with SEB (SEB group) and rats challenged with SEB and supplemented with spray-dried plasma (SEB-SDP group) or with Ig concentrate (SEB-IC group). The statistical analysis (one-way ANOVA followed by Bonferroni post-hoc test) showed that the SEB group has a higher TNFa concentration in the three tissues than controls and that both dietary supplements can reduce the expression of TNFa and stimulate the release of IL-10 (38) .…”

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

“…3, show that both SDP and IC reduce the SEB-induced release of pro-inflammatory cytokines, while increasing IL-10 concentration in GALT as well as at the systemic level. Interestingly, SDP can increase mucosal IL-10 concentration either in the presence or absence of an SEB challenge (38) . There are several mechanisms by which SDP and IC can modulate the intestinal immune response.…”

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

See 3 more Smart Citations

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

Pérez-Bosque

Moretó

2010

Proc. Nutr. Soc.

View full text Add to dashboard Cite

The epithelial barrier of the intestine and the gut-associated lymphoid tissue (GALT) protects the host against luminal pathogenic micro-organisms. This is important at weaning, when animals are exposed to infectious agents and stresses. We have developed a rat model of intestinal inflammation post weaning, based on the systemic administration ofStaphylococcus aureusenterotoxin B (SEB). Since the inflammatory response obtained is mild, the food intake pattern is not affected, which makes this model useful for studies of nutritional therapies for intestinal inflammatory disease. SEB increased T-lymphocytes in Peyer's patches and the number of activated T-lymphocytes in mesenteric lymph nodes (organized GALT). In the lamina propria, SEB increased activated T-lymphocytes as well as cytotoxic and natural killer-cell populations of the diffuse GALT. It also increased pro-inflammatory cytokines and inflammatory mediators in both Peyer's patches and mucosa. Rats given SEB had higher paracellular permeability to macromolecules, which was associated with a reduction in epithelial tightness. This model was used to examine whether dietary supplementation with spray-dried animal plasma proteins affects intestinal inflammation. Results showed that dietary plasma proteins can attenuate the mucosal immune response in both organized and diffuse GALT and that these effects are mediated by a reduction in the production of pro-inflammatory cytokines.

show abstract

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 80%

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

Section: Fig 1 Characteristics Of the Staphylococcus Aureus Enterotmentioning

confidence: 99%

See 2 more Smart Citations

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

Pérez-Bosque

Moretó

2010

Proc. Nutr. Soc.

View full text Add to dashboard Cite

show abstract

“…The extraction and retrotranscription of RNA were carried out as previously described (21). Real-time PCR was determined using a template of cDNA in a 20-l reaction containing 0.2 mol/l of each primer and 2 SyBr GreenER SuperMix (Life Technologies).…”

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability

Miró

Pérez-Bosque

Maijó

et al. 2013

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Miró L, Pérez-Bosque A, Maijó M, Amat C, Naftalin RJ, Moretó M. Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability. Am J Physiol Cell Physiol 304: C918-C926, 2013. First published March 6, 2013; doi:10.1152 doi:10. /ajpcell.00292.2012 show that raised aldosterone (Aldo) during low-Na adaptation regulates the growth of pericryptal myofibroblasts and reduces the permeability of the colonic epithelium. The aim of this study was to reproduce in vitro the in vivo condition of increased Aldo using human CCD-18Co myofibroblasts and T84 colonic epithelial cells to measure myofibroblast and epithelial proliferation and the expression of intercellular junction proteins. Proliferation was quantified by measuring 5-bromo-2=-deoxyuridine incorporation. The myofibroblast expression of EGF, VEGFa, and transforming growth factor-␤1 (TGF-␤1) was measured by real-time PCR and the expression of junctional complex proteins by Western blot. Aldo stimulated the proliferation of myofibroblasts by 70% (P Ͻ 0.05) and increased EGF mRNA expression by 30% (P Ͻ 0.05) without affecting VEGFa and TGF-␤1. EGF concentration in the incubation medium increased by 30% (P Ͻ 0.05) 24 h after Aldo addition, and these effects were prevented by the addition of spironolactone. Myofibroblast proliferation in response to Aldo was mediated by EGF receptor (EGFR) and involved both MAPKK and phosphatidylinositol 3-kinase pathways. When T84 cells were incubated with medium from myofibroblasts stimulated with Aldo (conditioned medium), the expression of ␤-catenin and claudin IV was increased by 30% (P Ͻ 0.05) and proliferation by 40% (P Ͻ 0.05). T84 proliferation decreased when ␣-EGF, or the EGFR antagonist AG1478, was present. Results in vivo indicate that rats fed a low-salt diet showed an increased expression of EGF and EGFR in the colonic mucosa. These results support the view that changes in colonic permeability during low-Na adaptation are mediated by the EGF secreted by myofibroblasts in response to raised Aldo. aldosterone; EGF; proliferation THE CRYPTS IN THE DESCENDING colon can absorb fluid despite a high hydraulic resistance in the lumen. The capacity to absorb a hypertonic absorbate requires Na pump activity and a physical barrier to Na movement from the pericryptal space into the submucosa and pericryptal capillaries to maintain a high osmotic pressure gradient across the crypt mucosa (19). This physical barrier is called the pericryptal sheath and is made up of a network of myofibroblast-like cells and the extracellular matrix surrounding the colonic crypts (13,19,22).Low-Na intake activates the renin-angiotensin-aldosterone system (RAAS), which increases both angiotensin II (ANG II) and aldosterone (Aldo) plasma concentration. Activation of the RAAS increases colonic fluid and Na absorption (31). These effects are partly due to the upregulation of the epithelial sodium channel (ENaC) and changes in the barrier function of the pericryptal sheath, as has been shown in both rat and mouse descending colonic...

show abstract

Microbial insight into dietary protein source affects intestinal function of pigs with intrauterine growth retardation

Zhou

et al. 2019

Eur J Nutr

View full text Add to dashboard Cite

Dietary Plasma Protein Supplements Prevent the Release of Mucosal Proinflammatory Mediators in Intestinal Inflammation in Rats

Cited by 62 publications

References 32 publications

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability

Microbial insight into dietary protein source affects intestinal function of pigs with intrauterine growth retardation

Contact Info

Product

Resources

About