Dietary Recombinant Phycoerythrin Modulates the Gut Microbiota of H22 Tumor-Bearing Mice

Abstract

Normal intestinal flora is widely involved in many functions of the host: nutritional metabolism; maintenance of intestinal microecological balance; regulation of intestinal endocrine function and nerve signal transduction; promotion of intestinal immune system development and maturation; inhibition of pathogenic bacteria growth and colonization, reduction of its invasion to intestinal mucosa, and so on. In recent years, more and more studies have shown that intestinal flora is closely related to the occurrence, development, and treatment of various tumors. It is indicated that recombinant phycoerythrin (RPE) has significant anti-tumor and immunomodulatory effects. However, little is known about the mechanism of the effect of oral (or intragastric) administration of RPE on gut microbiota in tumor-bearing animals. In this study, using high-throughput 16S rDNA sequencing, we examined the response of gut microbiota in H22-bearing mice to dietary RPE supplementation. The results showed that the abundance of beneficial bacteria in the mice intestinal flora decreased and that of the detrimental flora increased after inoculation with tumor cells (H22); following treatment with dietary RPE, the abundance of beneficial bacteria in the intestinal flora significantly increased and that of detrimental bacteria decreased. In this study, for the first time, it was demonstrated that dietary RPE could modulate the gut microbiota of the H22 bearing mice by increasing the abundance of beneficial bacteria and decreasing that of detrimental bacteria among intestinal bacteria, providing evidence for the mechanism by which bioactive proteins affect intestinal nutrition and disease resistance in animals.

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

“…16S rRNA amplicon sequencing could be used to determine all microorganisms’ genetic composition and community function in environmental samples ( Qi et al 2019 ). In our study, the results of 16S rRNA amplicon sequencing showed that the ACE index and Chao1 index slightly increased in the MGD group compared to the NC group, but there was no significant difference.…”

“…Candidatu_Saccharimonas has been associated with inflammatory diseases, such as gingivitis and other periodontal dysfunctions ( Cruz et al 2020 ). Alloprevotella can produce vitamin B1 and folic acid, and an increase in the abundance of Alloprevotella was associated with the improvement of intestinal disorders Seddik et al 2017 ; Qi et al 2019 ). Based on our results, the abundance of Alloprevotella in the LDG group was significantly higher than in the NC group.…”

The inhibitory effects of probiotics on colon cancer cells: in vitro and in vivo studies

“…The incidence of CRC has been associated with environmental and genetic factors, and has been linked with inflammatory bowel disease (IBD) and intestinal flora (11). Studies have shown that probiotics can regulate the microbiota of the intestinal tract and inhibit the progression of cancers (12). This suggests that there is a special connection between probiotics and CRC.…”

Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

“…Therefore, R-PE is regarded as a bioactive molecule with high value. It has been reported that R-PE has numerous biological activities, including antioxidant [9], anti-inflammatory [10], anti-aging [11], and immunomodulatory activity [12,13]. R-PE has excellent optical properties as a natural fluorescent protein [1,14].…”