Dietary red palm oil supplementation reduces myocardial infarct size in an isolated perfused rat heart model

Abstract

Background and AimsRecent studies have shown that dietary red palm oil (RPO) supplementation improves functional recovery following ischaemia/reperfusion in isolated hearts. The main aim of this study was to investigate the effects of dietary RPO supplementation on myocardial infarct size after ischaemia/reperfusion injury. The effects of dietary RPO supplementation on matrix metalloproteinase-2 (MMP2) activation and PKB/Akt phosphorylation were also investigated.Materials and methodsMale Wistar rats were divided into three groups and fed a standard rat chow diet (SRC), a SRC supplemented with RPO, or a SRC supplemented with sunflower oil (SFO), for a five week period, respectively. After the feeding period, hearts were excised and perfused on a Langendorff perfusion apparatus. Hearts were subjected to thirty minutes of normothermic global ischaemia and two hours of reperfusion. Infarct size was determined by triphenyltetrazolium chloride staining. Coronary effluent was collected for the first ten minutes of reperfusion in order to measure MMP2 activity by gelatin zymography.ResultsDietary RPO-supplementation decreased myocardial infarct size significantly when compared to the SRC-group and the SFO-supplemented group (9.1 ± 1.0% versus 30.2 ± 3.9% and 27.1 ± 2.4% respectively). Both dietary RPO- and SFO-supplementation were able to decrease MMP2 activity when compared to the SRC fed group. PKB/Akt phosphorylation (Thr 308) was found to be significantly higher in the dietary RPO supplemented group when compared to the SFO supplemented group at 10 minutes into reperfusion. There was, however, no significant changes observed in ERK phosphorylation.ConclusionsDietary RPO-supplementation was found to be more effective than SFO-supplementation in reducing myocardial infarct size after ischaemia/reperfusion injury. Both dietary RPO and SFO were able to reduce MMP2 activity, which suggests that MMP2 activity does not play a major role in protection offered by RPO. PKB/Akt phosphorylation may, however, be involved in RPO mediated protection.

Enzymatic degradation of biofilm by metalloprotease fromMicrobacteriumsp. SKS10

“…The activity of proteases in gel was detected using casein zymography (Bester et al, 2010). After electrophoresis, SDS-PAGE containing 0.1% casein was soaked in 2.5% (v/v) Triton X-100 at room temperature for 1 hour followed by overnight incubation in 50 mM Tris buffer (pH 8).…”

Bioactive Compounds in Red Palm Oil Can Modulate Mechanisms of Actions in In Vitro Anoxic Perfused Rat Hearts

“…A significant increase in cGMP levels was observed during the ischemic period. Bester et al (2010) have shown that a dietary intake of 7 mg kg À1 RPO could reduce infarct size. These findings suggest that RPO can also protect the heart in the ischemic period, but rather through a different mechanism involving NO-cGMP.…”

“…Initially, all studies by the Van Rooyen group (Bester et al, , 2010Engelbrecht et al, 2006;Esterhuyse et al, 2006;Kruger et al, 2007) on red palm oil supplementation in the ischemia/ reperfusion anoxic model were performed using a daily dose of 7 mg kg À1 diet in the rat model. Initially, all studies by the Van Rooyen group (Bester et al, , 2010Engelbrecht et al, 2006;Esterhuyse et al, 2006;Kruger et al, 2007) on red palm oil supplementation in the ischemia/ reperfusion anoxic model were performed using a daily dose of 7 mg kg À1 diet in the rat model.…”

Cardioprotective Effect of the Aqueous Extract of Lavender Flower against Myocardial Ischemia/Reperfusion Injury

“…We put the sliced hearts in TTC with the concentration of 1% to be incubated for 15 minutes and we turned over the hearts once in this process. When the TTC staining was over, the heart slices were dried with filter paper, and then these slices were covered in formalin solution for 15 minutes, Finally, these Journal of Chemistry 3 slices were immersed in phosphate buffer at 4 ∘ C (pH 7.4) [16]. Heart slices were digitally imaged using a Canon camera.…”