Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

Abstract

Selenium (Se), selenoprotein N (SelN) and selenoprotein W (SelW) play a crucial role in muscle disorders. Se status highly regulates selenoprotein mRNA levels. However, few attempts have been performed on the effect of dietary Se supplementation on muscle SelN and SelW mRNA levels in birds. To investigate the effects of Se on the regulation of SelN and SelW mRNA levels in muscle tissues, one-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0 mg/kg sodium selenite for 90 days. Muscle tissues (breast, flight, thigh, shank and cardiac muscles) were collected and examined for Se content and mRNA levels of SelN and SelW. Moreover, Selenophosphate synthetase-1 (SPS-1) and selenocysteine-synthase (SecS) mRNA levels were analyzed. Significant increases in SelN mRNA levels were obtained in breast, thigh and shank muscles treated with Se, with maximal effects at 3.0 mg Se/kg diet, but 2.0 mg Se/kg diet resulted in peak levels of Sel N mRNA in flight muscles. Changes in SelW mRNA abundance in thigh and shank muscles increased in response to Se supply. After reaching a maximal level, higher Se supplementation led to a reduction in both SelN and SelW mRNAs. However, SelN and SelW mRNA levels displayed a different expression pattern in different skeletal and cardiac muscles. Thus, it suggested that skeletal and cardiac muscles SelN and SelW mRNA levels were highly regulated by Se supplementation and different muscle tissues showed differential sensitivity. Moreover, Se supplementation also regulated the levels of SPS1 and SecS mRNAs. The mRNA levels of SPS1 and SecS were enhanced in the Se supplemented groups. These data indicate that Se regulates the expression of SelN and SelW gene and affect the mRNA levels of SecS and SPS1.

Effect of 2-hydroxy-4-methylselenobutanoic acid as a dietary selenium supplement to improve the selenium concentration of table eggs1

“…Selenium has been recognized as a nutritional essential trace element that is important in many biological processes in mammals and birds (Holben and Smith, 1999;Surai, 2000). It has a crucial role in embryonic and postnatal development (Surai, 2000;Fortier et al, 2012), immunity, reproduction, antioxidant system (Choct et al, 2004;Juniper et al, 2011), and muscle function (Ruan et al, 2012;Zhang et al, 2012) and is known as a natural antioxidant (Surai, 2002). In poultry as well as in other animal species, Se can be added to diets through its mineral or organic forms, which represent the crucial factor that determines its metabolic fate (Suzuki, 2005).…”

The role of selenium in nutrition – A review

“…Wilson et al, 1992;Holben & Smith, 1999). Thioredoxin reductase regulates cell growth(Mustacich & Powis, 2000), while selenoprotein N is responsible for normal muscle development(Zhang et al, 2012).…”

Association of maternal prenatal selenium concentration and preterm birth: a multicountry meta-analysis

“…28 The essential micronutrient Se, which exerts its antioxidant and anti-inflammatory properties in the form of selenoproteins such as glutathione peroxidase 3, selenoprotein P1 and thioredoxin reductase, has been shown to be protective in various inflammatory-based disease models. [29][30][31] A recent study in mice showed that Se in the form of selenoproteins played an indispensable role in uterine smooth muscle contractions, and the absence of any of these proteins affected the uterine contractility. 32 In vitro study of Se supplementation demonstrated that selenite suppresses key mediators involved in inflammation-induced activation of mediators involved in active labour in human fetal membranes and the myometrium.…”