Dietary supplementation with 25-hydroxycholecalciferol and phytase in growing-finishing pigs: II. Effects on intestinal antioxidant status, immunity and bone quality

Zhang, L.H.; Liu, H.S.; Liu, S.J.; Piao, Xiangshu

doi:10.1016/j.anifeedsci.2021.115065

Cited by 9 publications

(12 citation statements)

References 51 publications

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“…SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. Iodine value = (0.95 × C16:1) + (0.86 × C18:1) + (1.732 × C18:2) + (2.616 × C18:3) + (0.785 × C20:1) + (0.723 × C22:1) ( Zhang et al, 2021b ).…”

Section: Resultsmentioning

confidence: 99%

“…Blood (10 ml) was collected via the jugular vein, and the sample was centrifuged at 3000 × g for 10 min at 4°C after standing for 3 h, then collected the serum and stored at −20°C for analysis. On day 88, one barrow close to the average BW of each pen was humanly slaughtered after electrical stunning ( Zhang et al, 2021b ). The collecting methods of tissue samples refer to Zhang et al (2021a , b) .…”

Section: Methodsmentioning

confidence: 99%

“…On day 88, one barrow close to the average BW of each pen was humanly slaughtered after electrical stunning ( Zhang et al, 2021b ). The collecting methods of tissue samples refer to Zhang et al (2021a , b) . Immediately after death, mucosal samples were scraped from the middle parts of the duodenum, jejunum and ileum and stored at −80°C for analysis of antioxidant parameters.…”

Section: Methodsmentioning

confidence: 99%

“…According to the method of Zhang et al (2021b) , total RNA was extracted from longissimus dorsi using Trizol reagent (CWBIO Biotech Co., Beijing, China) and then reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa, Dalian, China). SYBR Green-based RT-PCR was performed using the ABI Prism 7,500 Sequence Detection System (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…In addition to this function, vitamin D 3 can improve the antioxidant status and immunity of pigs, promote the differentiation of the intestinal epithelial cells, and regulate the gut microbiota community ( Tian et al, 2016 ; Rey et al, 2020 ; Zhang and Piao, 2021 ; Zhang et al, 2022b ). Vitamin D 3 plays an important role in promoting antioxidant ability in the loin muscle of pigs, which contributes to extending shelf life and supporting meat quality ( Zhang et al, 2021b ). Importantly, it can also enhance dietary phytate P availability by promoting phytase activity in the intestine ( Zhang et al, 2022c ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary 25-hydroxycholecalciferol modulates gut microbiota and improves the growth, meat quality, and antioxidant status of growing-finishing pigs

Zhang

Long²,

Wang³

et al. 2023

Front. Microbiol.

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Introduction25-Hydroxycholecalciferol (25OHD3) is the active metabolite of regular vitamin D3in vivo, which has a stronger biological activity and is more easily absorbed by the intestine than regular vitamin D3. Our study aimed to detect the potential influences of 25OHD3 on pork quality, antioxidant status, and intestinal microbiota of growing-finishing pigs receiving low-phosphorus (P) diet.Methods and resultsForty pigs [initial body weight (BW): 49.42 ± 4.01 kg] were allocated into two groups including low-P diet (CON group) and low-P diet supplemented with 50 μg/kg 25OHD3 (25OHD3 group). The whole experiment lasted for 88 days, including phase 1 (day 1–28), phase 2 (day 29–60), and phase 3 (day 61–88). The results showed that 25OHD3 supplementation tended to decrease feed conversion ratio in phase 3 and overall phase in comparison with the CON group. 25OHD3 increased (p < 0.05) serum contents of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased (p < 0.05) serum bone-specific alkaline phosphatase level. 25OHD3 increased (p < 0.05) mucosal GSH-Px activity in the duodenum and ileum, and tended to increase redness value and the activities of total antioxidant capacity and SOD in longissimus dorsi. 25OHD3 significantly upregulated the mRNA level of copper/zinc superoxide dismutase, and tended to change the mRNA levels of nuclear factor E2-related factor 2 and kelch-like ECH-associated protein 1 in longissimus dorsi. Moreover, 25OHD3 supplementation decreased (p < 0.05) n-6/n-3 and iodine value in longissimus dorsi. For bone quality, 25OHD3 supplementation increased (p < 0.05) calcium content, bone mineral content, and breaking strength in the metacarpal bones. Moreover, the colonic abundance of Lactobacillus was significantly higher in pigs fed with 25OHD3, and exhibited a positive association with serum antioxidant status, pork quality, and bone characteristics.ConclusionOverall, the inclusion of 25OHD3 in low P diet partly improved production performance, meat quality, antioxidant capacity, bone properties, and gut microbiota composition of growing-finishing pigs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary 25-hydroxycholecalciferol modulates gut microbiota and improves the growth, meat quality, and antioxidant status of growing-finishing pigs

Zhang

Long²,

Wang³

et al. 2023

Front. Microbiol.

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Rosemary leaf powder improves egg quality, antioxidant status, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens

Zhang,

Ge,

Gao

et al. 2024

Animal Nutrition

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β-mannanase supplemented in diets saved 85 to 100 kcal of metabolizable energy/kg, supporting growth performance and improving nutrient digestibility in grower pigs

Azevedo

Rupolo

Cordeiro

et al. 2023

Sci Rep

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The effects of β-mannanase supplementation in metabolizable energy (ME)-reduced diets containing xylanase-phytase were investigated on growth performance, fecal score, ultra-sounded backfat thickness and loin depth, blood profile, apparent total tract digestibility (ATTD), digesta passage rate, and fecal microbiome in grower pigs (n = 40, 26.09 ± 0.96 kg) randomly assigned within 4 treatments: a control diet containing isolated phytase and xylanase valued at 40 kcal of ME/kg (CD0), CD0 + β-mannanase (0.3 g/kg valued at 30 kcal of ME/kg) (CD70), CD0 + β-mannanase (0.3 g/kg valued at 45 kcal of ME/kg) (CD85), and CD0 + β-mannanase (0.3 g/kg valued at 60 kcal of ME/kg) (CD100). Growth performance was not affected in pigs fed ME-reduced diets containing β-mannanase. Pigs with CD100 had lower serum IL-1β concentration, and higher IL-10 was observed in pigs on CD0 than those fed β-mannanase. Coefficients of ATTD, and ATTD of DM and CP were higher in animals fed CD85 or CD100. Pigs with CD85 had higher alpha diversity richness but lower Firmicutes:Bacteroidota ratio. Acidaminococcaceae and Ruminococcaceae were more abundant in pigs fed CD0, but lower for Christensenellaceae NSJ-63 and NSJ-63 sp014384805. Pigs in CD85 showed higher Bacteroidaceae and Prevotella abundance, and lower for Streptococcaceae and Streptococcus. In conclusion, supplementation of β-mannanase in diets containing xylanase-phytase saved 85 to 100 kcal of ME/kg by supporting growth performance and improving nutrient digestibility in grower pigs.

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Dietary supplementation with 25-hydroxycholecalciferol and phytase in growing-finishing pigs: II. Effects on intestinal antioxidant status, immunity and bone quality

Cited by 9 publications

References 51 publications

Dietary 25-hydroxycholecalciferol modulates gut microbiota and improves the growth, meat quality, and antioxidant status of growing-finishing pigs

Dietary 25-hydroxycholecalciferol modulates gut microbiota and improves the growth, meat quality, and antioxidant status of growing-finishing pigs

Rosemary leaf powder improves egg quality, antioxidant status, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens

β-mannanase supplemented in diets saved 85 to 100 kcal of metabolizable energy/kg, supporting growth performance and improving nutrient digestibility in grower pigs

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