Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows

Wang, Yue; Nan, Xuemei; Zhao, Yiguang; Jiang, Linshu; Wang, Hui; Hua, Dengke; Zhang, Fan; Wang, Yapin; Li, Jun; Yao, Junhu; Xiong, Benhai

doi:10.1016/j.aninu.2021.09.007

Cited by 36 publications

(32 citation statements)

References 58 publications

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“…Previous studies have suggested that extracts from natural plants could affect the composition of rumen microorganisms in ruminants ( Jiao et al, 2021 ; Wang et al, 2021 ). Bacteroidetes and Firmicutes are the predominant phyla in the ruminal microbial community, which is consistent with our results ( Scharen et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Allium mongolicum Regel ethanol extract on three flavor-related rumen branched-chain fatty acids, rumen fermentation and rumen bacteria in lambs

et al. 2022

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The objective of this study was to evaluate the effect of Allium mongolicum Regel ethanol extract (AME) on the concentration of three branched-chain fatty acids (BCFAs) related to flavor, fermentation parameters and the bacteria and their correlations in the rumen of lambs. A total of thirty 3-month-old male, Small-tailed Han sheep (33.60 ± 1.23 kg) were randomly distributed into 2 groups as follows: control group (CON) was fed a basal diet and AME group was fed a basal diet supplemented with 2.8 g⋅lamb–1⋅d–1A. mongolicum Regel ethanol extract. AME supplementation decreased (P = 0.022) 4-methyloctanoic acid (MOA) content and tended to lower (P = 0.055) 4-methylnonanoic acid (MNA) content in the rumen. Compared to CON group, the ruminal concentrations of valerate and isovalerate were higher (P = 0.046 and P = 0.024, respectively), and propionate was lower (P = 0.020) in the AME group. At the phylum level, the AME group had a lower abundance of Bacteroidetes (P = 0.014) and a higher abundance of Firmicutes (P = 0.020) than the CON group. At the genus level, the relative abundances of Prevotella (P = 0.001), Christensenellaceae_R-7_group (P = 0.003), Succiniclasticum (P = 0.004), and Selenomonas (P = 0.001) were significantly lower in the AME group than in the CON group, while the relative abundances of Ruminococcus (P < 0.001), Quinella (P = 0.013), and Lachnospiraceae_XPB1014_group (P = 0.001) were significantly higher. The relative abundances of Prevotella (P = 0.029, R = 0.685; P = 0.009, R = 0.770), Christensenellaceae_R-7_group (P = 0.019, R = 0.721; P = 0.029, R = 0.685), and Succiniclasticum (P = 0.002, R = 0.842; P = 0.001, R = 0.879) was positively correlated with MOA and MNA levels, and the relative abundance of Lachnospiraceae_XPB1014_group (P = 0.033, R = −0.673) was negatively correlated with MOA. The relative abundance of Christensenellaceae_R-7_group (P = 0.014, R = −0.744) and Prevotellaceae_UCG-003 (P = 0.023, R = −0.706) correlated negatively with the EOA content. In conclusion, these findings suggest that the AME affected the concentration of BCFAs, fermentation parameters and the rumen bacteria in the rumen of lambs.

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Section: Discussionmentioning

confidence: 99%

Effects of Allium mongolicum Regel ethanol extract on three flavor-related rumen branched-chain fatty acids, rumen fermentation and rumen bacteria in lambs

et al. 2022

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“…This is achieved by increasing the molar proportion of acetate, the ratio of acetate to propionate and by increasing the molar proportion of propionate in the rumen fluid of Holstein cows. Wang et al (2021a) reported a significant increase in the milk production of Holstein cows through the addition of inulin, which is due, among other factors, to an increase in the production and concentration of rumen propionate. Short-chain fatty acids (6-14 carbon fatty acids) in milk are formed mainly by de novo synthesis from acetate and butyrate produced in mammary gland epithelial cells in the rumen, and 16 carbon fatty acids in milk are both produced by de novo synthesis and fatty acids absorbed from the blood (Liu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of supplementary inulin on ewes milk composition and rumen fermentation parameters

Paya

Giannenas

Taghizadeh

et al. 2022

Journal of Dairy Research

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This experiment aimed to investigate the effects of inulin supplementation on milk production and composition, feed intake, nutrient digestibility and rumen fermentation parameters in lactating ewes. The experimental treatments were (1) control group (basal diet), (2) basal diet plus 2% inulin (w/w) and (3) basal diet plus 4% inulin (w/w). The experiment was carried out for 21 d in a fully randomized design involving eighteen Ghezel ewes. Production and composition (percentages of fat, protein, lactose and fat-free solids and fatty acid profiles) of milk were measured. Faeces were collected in the last 3 days of the experiment to determine digestibility. On the last day of the experiment, rumen fluid samples were taken from the esophagus 3 h after feeding and fermentation parameters (pH, ammonia nitrogen (N-NH3), volatile fatty acids (VFA) and protozoal population) were examined. Daily milk production was not significantly affected by inulin supplementation, but the fat and protein content of the milk was increased whilst urea nitrogen (MUN) and unsaturated fatty acids were decreased (P < 0.05). The dry matter (DM) intake results showed that there was no significant difference between different diets. The highest digestibility of DM and NDF belonged to the inulin fed group (P < 0.05). Inulin consumption numerically increased the pH of the rumen fluid of the animals and significantly decreased the rumen N-NH3 value (P < 0.05). Inulin supplementation also significantly increased total VFA, acetate, and butyrate levels (P < 0.05). In general, it can be concluded that inulin supplementation can improve rumen fermentation, DM and NDF digestibility,as well as compositional aspects of the ewe's milk production.

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“…Milk lipid composition was further analyzed according to the acetyl chloride-methanol methyl esterification method presented by Wang et al ( 19 ). Parameters including saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) were measured.…”

Section: Methodsmentioning

confidence: 99%

Microbial and metabolomic insights into the bovine lipometabolic responses of rumen and mammary gland to zymolytic small peptide supplementation

Liu

Xiao²,

Pu³

et al. 2022

Front. Vet. Sci.

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Small peptides provide the easily utilized nitrogen for rumen microbial and promote acetate generation for milk fat synthesis. However, the impacts of peptide supplements on lipometabolic processes were still unclear. Therefore, a total of 800 multiparous dairy herds (with an average live weight of 667.6 ± 39.4 kg, an average lactation of 89.3 ± 18.8 days, and an average calving parity of 2.76 ± 0.47) were randomly allocated to the control (CON) and the small peptide (SP) supplement (100 g/day for each cow) treatments, respectively. A 35-day-long feeding procedure that includes a 7-day-long pretreatment test and a 28-day-long treatment test was followed for all cows. Dry matter intake (DMI) was recorded every day and calculated by the deviation between the supply and residue, while the daily milk production was automatically recorded through the rotary milking facilities. Milk samples were collected from each replicate on the last day, followed by the milk quality and milk lipid composition measurement. Rumen fluid samples were collected on the last day through esophageal tubing 3 h after morning feeding for the determination of the underlying mechanism of the small peptide on lipid metabolism through the measurement of rumen lipometabolic-related metabolites and rumen bacterial communities. Results indicated that dry matter intake showed an increasing trend, while milk production and the milk fat content remarkably increased after SP supplement (P < 0.05). Further detailed detection showed the mainly increased milk composition focused on monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA). Acetate-producing microbes, such as Acetitomaculum, Bifidobacterium, Succiniclasticum, and Succinivibrio, and butyrate-producing microbes, such as Shuttleworthia and Saccharofermentans, significantly proliferated, which causatively brought the increased ruminal content of acetate, isobutyrate, and butyrate after SP supplement (P < 0.05) compared with CON. Lipometabolic metabolites such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), triacylglycerol (TG), and Acetyl-CoA also significantly increased after SP supplement. In summary, SP supplements help to increase milk fat content through the proliferation of rumen bacterial communities, which provided more acetate and butyrate for milk fat synthesis combined with the promotion of ruminal lipometabolism.

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Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows

Cited by 36 publications

References 58 publications

Effects of Allium mongolicum Regel ethanol extract on three flavor-related rumen branched-chain fatty acids, rumen fermentation and rumen bacteria in lambs

Effects of Allium mongolicum Regel ethanol extract on three flavor-related rumen branched-chain fatty acids, rumen fermentation and rumen bacteria in lambs

Effects of supplementary inulin on ewes milk composition and rumen fermentation parameters

Microbial and metabolomic insights into the bovine lipometabolic responses of rumen and mammary gland to zymolytic small peptide supplementation

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