Diets enriched with cranberry beans alter the microbiota and mitigate colitis severity and associated inflammation

Monk, Jennifer M.; Lepp, Dion; Zhang, Claire P.; Wu, Wenqing; Zarepoor, Leila; Lu, Jenifer T.; Pauls, K. Peter; Tsao, Rong; Wood, Geoffrey A.; Robinson, Lindsay E.; Power, Krista A.

doi:10.1016/j.jnutbio.2015.10.014

Cited by 96 publications

(77 citation statements)

References 110 publications

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“…Our study shows that supplementation of GP accelerated the recovery from colitis symptoms in the HFD-fed mice as evident from decreased body weight loss and a lower fecal blood score during the recovery stage. In support of our findings, supplementation of dietary white and dark kidney beans as well as cranberry beans reduced colitis severity by reducing body weight loss, fecal blood score, and resultant DAI score in DSS-induced colitis mice [15,16]. Similarly, dietary supplementation of soybeans Bowman–Birk inhibitor concentrate reduced the severity of DSS-colitis by suppressing inflammation in the colon and improving the recovery following DSS-induction [14].…”

Section: Discussionsupporting

confidence: 67%

“…In agreement, the current study found that DSS-induction enhanced both the neutrophil infiltration and MCP-1 expression in the colon of HFD-fed mice, which were mitigated by GP supplementation. Inline, dietary white and dark kidney beans, as well as cranberry bean supplements, reduced the mRNA expression of MCP-1 in the colon of DSS-colitis mice [15,16]. …”

Section: Discussionmentioning

confidence: 99%

“…In the current study, elevated levels of IL-6, IL-17, IFN-γ, iNOS, and COX-2 caused by DSS-induction were ameliorated by GP supplementation in the colon of HFD-fed mice. Consistently, dietary white and dark kidney beans reduced the mRNA expression of IL-6 [15], and cranberry bean supplementation reduced the IL-6 protein in the colon along with reduced serum IL-6, IL-17, and IFN-γ in DSS-induced colitis [16]. The down-regulation of inflammatory cascades and oxidative stress by GP supplementation can be partially explained by the low neutrophil infiltration into the colon of HFD-fed DSS-colitis mice.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, dietary chickpea supplementation increased colon mucus content, mRNA expression of MUC-2, and differentiation marker Klf4 with enhanced gut barrier integrity and reduced inflammation in healthy unchallenged mice [13]. In DSS-induced colitis, dietary white and dark kidney beans, as well as dietary cranberry bean supplementation, enhanced the mRNA expression of MUC-2 and Tff3, and mitigated the severity of colitis and associated inflammation [15,16]. Consistent with these observations as well as improved colitis symptoms, GP supplementation improved both protein and gene expression of MUC-2 in HFD-fed DSS-induced mice, associated with the enhanced expression of goblet cell differentiation markers in the colon.…”

Section: Discussionmentioning

confidence: 99%

“…Legumes and pulses, including peas, are rich in fiber and other phytonutrients that boost beneficial intestinal microbiota [12], producing short chain fatty acids (SCFA) and promoting epithelial barrier integrity [13]. Further, dietary soybean Bowman–Birk inhibitor concentrate [14], white and dark kidney beans [15], and cranberry bean supplements [16] suppressed colonic inflammation and reduced the severity of DSS-induced colitis in mice. Consistently, pea seed albumin extract ameliorated DSS-induced colitis in mice by reducing the expression of inflammatory markers in colonic tissues [17].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Green Pea Protects against DSS-Induced Colitis in Mice Challenged with High-Fat Diet

et al. 2017

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Obesity is a risk factor for developing inflammatory bowel disease. Pea is unique with its high content of dietary fiber, polyphenolics, and glycoproteins, all of which are known to be health beneficial. We aimed to investigate the impact of green pea (GP) supplementation on the susceptibility of high-fat diet (HFD)-fed mice to dextran sulfate sodium (DSS)-induced colitis. Six-week-old C57BL/6J female mice were fed a 45% HFD or HFD supplemented with 10% GP. After 7-week dietary supplementation, colitis was induced by adding 2.5% DSS in drinking water for 7 days followed by a 7-day recovery period. GP supplementation ameliorated the disease activity index score in HFD-fed mice during the recovery stage, and reduced neutrophil infiltration, mRNA expression of monocyte chemoattractant protein-1 (MCP-1) and inflammatory markers interleukin (IL)-6, cyclooxygenase-2 (COX-2), IL-17, interferon-γ (IFN-γ), and inducible nitric oxide synthase (iNOS) in HFD-fed mice. Further, GP supplementation increased mucin 2 content and mRNA expression of goblet cell differentiation markers including Trefoil factor 3 (Tff3), Krüppel-like factor 4 (Klf4), and SAM pointed domain ETS factor 1 (Spdef1) in HFD-fed mice. In addition, GP ameliorated endoplasmic reticulum (ER) stress as indicated by the reduced expression of Activating transcription factor-6 (ATF-6) protein and its target genes chaperone protein glucose-regulated protein 78 (Grp78), the CCAAT-enhancer-binding protein homologous protein (CHOP), the ER degradation-enhancing α-mannosidase-like 1 protein (Edem1), and the X-box binding protein 1 (Xbp1) in HFD-fed mice. In conclusion, GP supplementation ameliorated the severity of DSS-induced colitis in HFD-fed mice, which was associated with the suppression of inflammation, mucin depletion, and ER stress in the colon.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%