Diferulate and lignin formation is related tobiochemical differences of wall-bound peroxidases

González, Luis; Rojas, Mireya; Pérez, Francisco J.

doi:10.1016/s0031-9422(98)00611-6

Cited by 31 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…POD assay was based on a method described by Gonzales et al (1999). The reaction mixture contained 0.1 M NaH 2 PO 4 /Na 2 HPO 4 buffer (pH 6.0), 20 mM guaiacol solution and 0.1 % (v/v) H 2 O 2 and diluted enzyme extract (1:10).…”

Section: Enzyme Extraction and Assaysmentioning

confidence: 99%

Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell cultures

Simic

Tusevski

Maury

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Hypericum perforatum cell suspensions were evaluated for their growth, phenylpropanoid and naphtodianthrone productions, and antioxidant activity after treatments with fungal elicitors Fusarium oxysporum, Phoma exigua and Botrytis cinerea. Elicited cells displayed a reduced biomass production, a rapid stimulation of secondary metabolites production and a modification of cell redox state compared to control. Cells responded strongly towards the applied elicitors through the enhanced production of naphtodianthrones. Hypericin and pseudohypericin production was significantly increased (up to fourfold) in the early growth phase and remained stable all along the post-elicitation period. Significant increase in contents of total phenolics, total flavonoids and total anthocyanins was observed during the entire period of cultivation, while total flavanols were enhanced at the end of post-elicitation. The enzymatic activities of phenylalanine ammonia lyase and chalcone isomerase were remarkably elevated in elicited cells confirming a strong activation of phenylpropanoid/flavonoid pathways. The fingerprint profile of Fourier transform infrared spectroscopy spectra from the cell walls showed a little variation in lignin accumulation between elicited and control samples. With regards to the antioxidant state, an early up-regulation of peroxidase activity was observed in elicited cells, whereas non-enzymatic properties and catalase activity were enhanced at the end of post-elicitation. These findings suggest the involvement of an efficient antioxidant defense system in the adaptive response of cells to fungal elicitation. Altogether, these results indicated that H. perforatum elicited cells represent a promising experimental system for scaleup production of naphtodianthrones for medicinal uses.

show abstract

Section: Enzyme Extraction and Assaysmentioning

confidence: 99%

Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell cultures

Simic

Tusevski

Maury

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…The POD assay was based on a method described by Gonzales et al (1999). The reaction mixture contained 0.1 M NaH 2 PO 4 /Na 2 HPO 4 buffer (pH 6.0), 20 mM guaiacol solution and 0.1 % (v/v) H 2 O 2 and diluted enzyme extract (1:10).…”

Section: Enzyme Extraction and Assaysmentioning

confidence: 99%

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Simic

Tusevski

Maury

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Hypericum perforatum cell suspensions were evaluated for their growth, phenylpropanoid and naphtodianthrone productions, and antioxidant activity after treatments with polysaccharide elicitors chitin (CHI), pectin (PEC) and dextran (DEX). Polysaccharide elicitation of cell cultures showed a considerable improving effect on phenylpropanoid production (phenolics, flavonoids, flavanols and anthocyanins) during the post-elicitation period. Even that enhanced phenylpropanoid production was related to growth suppression of CHI elicited cells, PEC and DEX were presented as effective elicitors without loss of cell biomass. Phenylpropanoid accumulation in elicited cells was correlated to markedly higher enzyme activities of phenylalanine ammonia lyase and chalcone-flavanone isomerase. Cell cultures were found to respond rapidly towards the applied elicitors through an early stimulation of both naphtodiantrones, hypericin (HYP) and pseudohypericin (PHYP). The CHI was proposed as a much more prominent elicitor in stimulation of HYP and PHYP levels in cell suspensions. All tested polysaccharide elicitors stimulated non-enzymatic antioxidant properties in cell extracts indicating the potential role of phenolic compounds as effective antioxidants. With regards to the antioxidant enzymes, an up-regulation of peroxidase and catalase activities was observed in PEC and DEX elicited cells, whereas CHI did not give any stimulatory effect on enzymatic activities. These findings suggest the involvement of an efficient antioxidant defense system in the adaptive response of cells to polysaccharide elicitation. Altogether, these results indicated that H. perforatum cells elicited with polysaccharides represent a promising experimental system for enhanced production of phenylpropanoids and naphtodianthrones.

show abstract

“…The samples were analyzed by reversed-phase HPLC using a Shimadzu model SCL-6B system equipped with a Mightysil RP-18 GP column (250 × 4.6 mm, Kanto Chemical Co., Tokyo, Japan) and a UV detector (Shimadzu, model SPD-6A), according to the method of González et al (1999) with following modifi cations. The fl ow rate was 1 mL min -1 and the elution was performed with an acetonitrile convex gradient against 3% acetic acid at 50˚C.…”

Section: Determination Of Phenolic Acidsmentioning

confidence: 99%

Changes in Cell Wall-Bound Phenolic Acids in the Internodes of Submerged Floating Rice

Azuma

Okita

Nanmori

et al. 2005

Plant Production Science

View full text Add to dashboard Cite

Diferulate and lignin formation is related tobiochemical differences of wall-bound peroxidases

Cited by 31 publications

References 22 publications

Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell cultures

Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell cultures

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Changes in Cell Wall-Bound Phenolic Acids in the Internodes of Submerged Floating Rice

Contact Info

Product

Resources

About