Difference in Actions of Harmine on the Oxidations of Serotonin and Tyramine by Beef Brain Mitochondrial Mao

Yasuhara, Hajime; Sho, Sadayuki; Kamijo, Keita

doi:10.1254/jjp.22.439

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…Nevertheless, it seems that it is the first time that such an impressive capacity of phenelzine in inhibiting human SSAO is reported for a native, tissue-bound form (neither recombinant nor purified). Figure 7 also shows a greater inhibition of the oxidation of tyramine than benzylamine by harmine, an historical inhibitor of MAO, able to limit the degradation of tyramine, serotonin [46], and kynuramine [44], that is, having more selectivity for MAO-A than for MAO-B (in accordance with the preponderant MAO-A on MAO-B in human WAT ( [24]). Hence, this is the first time, at least to our knowledge, that data evidence the selectivity of harmine towards MAO relative to SSAO inhibition, as this β-carboline was inhibiting the production of neosynthesized radiolabeled benzaldehyde up to 50% only in the millimolar range whereas it deeply inhibited tyramine oxidation at 100 nM.…”

Section: Interactions Of Opipramol and Other Psychotropes With Adiposmentioning

confidence: 57%

Opipramol Inhibits Lipolysis in Human Adipocytes without Altering Glucose Uptake and Differently from Antipsychotic and Antidepressant Drugs with Adverse Effects on Body Weight Control

et al. 2020

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Treatment with several antipsychotic drugs exhibits a tendency to induce weight gain and diabetic complications. The proposed mechanisms by which the atypical antipsychotic drug olanzapine increases body weight include central dysregulations leading to hyperphagia and direct peripheral impairment of fat cell lipolysis. Several investigations have reproduced in vitro direct actions of antipsychotics on rodent adipocytes, cultured preadipocytes, or human adipose tissue-derived stem cells. However, to our knowledge, no such direct action has been described in human mature adipocytes. The aim of the present study was to compare in human adipocytes the putative direct alterations of lipolysis by antipsychotics (haloperidol, olanzapine, ziprazidone, risperidone), antidepressants (pargyline, phenelzine), or anxiolytics (opipramol). Lipolytic responses to the tested drugs, and to recognized lipolytic (e.g., isoprenaline) or antilipolytic agents (e.g., insulin) were determined, together with glucose transport and amine oxidase activities in abdominal subcutaneous adipocytes from individuals undergoing plastic surgery. None of the tested drugs were lipolytic. Surprisingly, only opipramol exhibited substantial antilipolytic properties in the micromolar to millimolar range. An opipramol antilipolytic effect was evident against isoprenaline-, forskolin-, or atrial natriuretic peptide-stimulated lipolysis. Opipramol did not impair insulin activation of glucose transport but inhibited monoamine oxidase (MAO) activity to the same extent as antidepressants recognized as MAO inhibitors (pargyline, harmine, or phenelzine), whereas antipsychotics were inefficient. Considering its unique properties, opipramol, which is not associated with weight gain in treated patients, is a good candidate for drug repurposing because it limits exaggerated lipolysis, prevents hydrogen peroxide release by amine oxidases in adipocytes, and is thereby of potential use to limit lipotoxicity and oxidative stress, two deleterious complications of diabetes and obesity.

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Section: Interactions Of Opipramol and Other Psychotropes With Adiposmentioning

confidence: 57%

Opipramol Inhibits Lipolysis in Human Adipocytes without Altering Glucose Uptake and Differently from Antipsychotic and Antidepressant Drugs with Adverse Effects on Body Weight Control

et al. 2020

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“…Ayahuasca’s effects are derived from the combined action of β-carbolines (harmaline, harmine and tetrahydroharmine) which act as reversible monoamine oxidase inhibitor (MAOI), with N,N-dimethyltryptamine (DMT), an indole alkaloid similar to serotonin (5-HT). The inhibition process of the MAO caused by the β-carbolines results in a higher oral bioavailability of DMT, which acts mainly as an agonist of the 5-HT receptors (Fuller et al, 1970; Yasuhara et al, 1972; McKenna et al, 1984; McKenna, 2004). As a result, DMT promotes similar effects as the 5-HT itself (Rabin et al, 2002) and together with MAOI leads to increased BDNF levels in humans (dos Santos and Hallak, 2017), which seems to be related to amelioration of some psychological disease symptoms such as anxiety, obsessive-compulsive behavior and depression (Bouso et al, 2012; Cai et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

Lobão-Soares

Eduardo-da-Silva

Amarilha

et al. 2018

Front. Behav. Neurosci.

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Ayahuasca is a psychoactive brew traditionally used in shamanistic and vegetalistic rituals and has recently received lot of attention due to potential cognitive benefits. Ayahuasca effects are caused by the synergistic interaction of β-carbolines (harmine, harmaline and tetrahydroarmine) contained in Banisteriopsis caapi stalks combined with the N,N-dimethyltryptamine (DMT) from Psychotria viridis leaves, a potent agonist to serotonin (5-HT) receptors. The present study approaches the effects of chronic and acute exposure to two Ayahuasca concentrations (0.1 and 0.5 ml/L) on the cognitive ability to discriminate objects in a one-trial learning task in zebrafish. Based on the combination of concentrations and exposure regimens, we divided adult zebrafish in five treatment groups: acute 0.1 and 0.5 ml/L, chronic 0.1 and 0.5 ml/L, and control 0.0 (n = 20 for each group). Then we tested them in a memory task of object discrimination. Acute Ayahuasca exposed groups performed similarly to the control group, however chronically treated fish (13 days) presented both impaired discriminative performance and locomotor alterations. Overall, these results indicate that Ayahuasca is a potent psychoactive drug that, in chronic exposure, negatively affects mnemonic parameters in zebrafish. In single exposure it does not affects cognitive performance, but the higher concentration (0.5) affected locomotion. Moreover, we reinforce the importance of the zebrafish for behavioral pharmacological studies of drug screening, in special to psychedelic drug research.

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Some aspects of monoamine oxidase activity in brain

Achee

Gabay

Tipton

1977

Progress in Neurobiology

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Difference in Actions of Harmine on the Oxidations of Serotonin and Tyramine by Beef Brain Mitochondrial Mao

Cited by 5 publications

References 2 publications

Opipramol Inhibits Lipolysis in Human Adipocytes without Altering Glucose Uptake and Differently from Antipsychotic and Antidepressant Drugs with Adverse Effects on Body Weight Control

Opipramol Inhibits Lipolysis in Human Adipocytes without Altering Glucose Uptake and Differently from Antipsychotic and Antidepressant Drugs with Adverse Effects on Body Weight Control

It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

Some aspects of monoamine oxidase activity in brain

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