Difference in an intermolecular disulfide-bond between two highly homologous serum proteins Leg1a and Leg1b implicates their functional differentiation

Wang, Jinyang; Bai, Yun; Xie, Aixuan; Huang, Heping; Hu, Minjie; Peng, Jinrong

doi:10.1016/j.bbrc.2021.09.045

Cited by 2 publications

(4 citation statements)

References 21 publications

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“…To find out whether the HDGCR is activated in the leg1a or leg1b deleterious mutants, we took the advantage of the PTC-bearing leg1a zju1 single (with a 13 bp insertion in the exon 1 of leg1a ), leg1b zju1 single (with a 14 bp deletion in the exon 2 of leg1b ) and leg1a zju3 leg1b zju1 double (where leg1a zju3 harbors a 7 bp deletion in leg1a ) mutant lines available to our laboratory (Supplementary Fig. S1 ) 21 , 22 . leg1a zju1/zju1 single ( leg1a_mu ), leg1b zju1/zju1 single ( leg1b_mu ) and leg1a zju3/zju3 leg1b zju1/zju1 double ( leg1_dm ) homozygous mutants were all viable and fertile.…”

Section: Resultsmentioning

confidence: 99%

“…The zebrafish ( Danio rerio ) AB strain was used as WT in this study. The leg1a zju1 (with 13 bp insertion in the exon 1 of leg1a ) single, leg1b zju1 (with 14 bp deletion in the exon 2 of leg1b ) single, and leg1a zju3 leg1b zju1 double ( leg1a zju3 harboring a 7 bp deletion in leg1a ) mutant lines were in the AB background and were generated as previously described 21 , 22 . The upf3a −/− and upf1 −/− mutant lines were in the Tübingen background and were generated as described 13 .…”

Section: Methodsmentioning

confidence: 99%

“…For a cross between homozygous parents, more than 23 embryos were used. For a cross between heterozygous parents, more than 100 embryos were obtained and were genotyped using gene-specific primers (Supplementary Table S22 ) to obtain the corresponding homozygous mutant embryos as previously described 13 , 22 . All sets of experiments were repeated at least three times except the ULI-NChIP-seq which was performed with two repeats.…”

Section: Methodsmentioning

confidence: 99%

“…Liver-enriched gene 1 (Leg1) represents a protein family highly conserved in vertebrates. It is characterized by a single domain of unknown function 781 (DUF781) [19][20][21][22] . In zebrafish, Leg1 is a liver-produced novel serum protein 20 , whereas, in the platypus and echidna, the Leg1 family member known as Monotreme Lactation Protein (MLP) is a component of milk that may function as an antimicrobial protein 23,24 .…”

Section: Introductionmentioning

confidence: 99%

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Upf3a but not Upf1 mediates the genetic compensation response induced by leg1 deleterious mutations in an H3K4me3-independent manner

Xie

Wang

et al. 2023

Cell Discov

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Genetic compensation responses (GCRs) can be induced by deleterious mutations in living organisms in order to maintain genetic robustness. One type of GCRs, homology-dependent GCR (HDGCR), involves transcriptional activation of one or more homologous genes related to the mutated gene. In zebrafish, ~80% of the genetic mutants produced by gene editing technology failed to show obvious phenotypes. The HDGCR has been proposed to be one of the main reasons for this phenomenon. It is triggered by mutant mRNA bearing a premature termination codon and has been suggested to depend on components of both the nonsense mRNA-mediated degradation (NMD) pathway and the complex of proteins associated with Set1 (COMPASS). However, exactly which specific NMD factor is required for HDGCR remains disputed. Here, zebrafish leg1 deleterious mutants are adopted as a model to distinguish the role of the NMD factors Upf1 and Upf3a in HDGCR. Four single mutant lines and three double mutant lines were produced. The RNA-seq data from 71 samples and the ULI-NChIP-seq data from 8 samples were then analyzed to study the HDGCR in leg1 mutants. Our results provide strong evidence that Upf3a, but not Upf1, is essential for the HDGCR induced by nonsense mutations in leg1 genes where H3K4me3 enrichment appears not to be a prerequisite. We also show that Upf3a is responsible for correcting the expression of hundreds of genes that would otherwise be dysregulated in the leg1 deleterious mutant.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Upf3a but not Upf1 mediates the genetic compensation response induced by leg1 deleterious mutations in an H3K4me3-independent manner

Xie

Wang

et al. 2023

Cell Discov

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The Impact of High-Intensity Ultrasound-Assisted Extraction on the Structural and Functional Properties of Hempseed Protein Isolate (HPI)

Yao

et al. 2023

Foods

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Hempseed protein has become a promising candidate as a future alternative protein source due to its high nutritional value. In the current study, hempseed protein isolate (HPI) was obtained using ultrasonic-assisted extraction with the aim to improve the functionality of HPI via protein structure modification. The solubility of HPI could be improved twofold under 20 kHz ultrasound processing compared to conventional alkaline extraction-isoelectric point precipitation. The protein solubility was gradually enhanced as the ultrasonic power improved, whereas excessive ultrasound intensity would cause a decline in protein solubility. Ultrasonic processing was found to have beneficial effects on the other functionalities of the extracted HPI, such as emulsifying and foaming properties. This improvement can be ascribed to the physical effects of acoustic cavitation that changed the secondary and tertiary structures of the protein to enhance surface hydrophobicity and decrease the particle size of the extracted protein aggregates. In addition, more available thiols were observed in US-treated samples, which could be another reason for improved functionality. However, the results of this study also revealed that prolonged high-power ultrasound exposure may eventually have a detrimental impact on HPI functional properties due to protein aggregation. Overall, this study suggests that high intensity ultrasound can enhance the functionality of HPI, which may ultimately improve its value in HPI-based food products.

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Difference in an intermolecular disulfide-bond between two highly homologous serum proteins Leg1a and Leg1b implicates their functional differentiation

Cited by 2 publications

References 21 publications

Upf3a but not Upf1 mediates the genetic compensation response induced by leg1 deleterious mutations in an H3K4me3-independent manner

Upf3a but not Upf1 mediates the genetic compensation response induced by leg1 deleterious mutations in an H3K4me3-independent manner

The Impact of High-Intensity Ultrasound-Assisted Extraction on the Structural and Functional Properties of Hempseed Protein Isolate (HPI)

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