Difference in localization of eosinophils and mast cells in the bovine ovary

Abstract

The bovine ovary contains a considerable number of leucocytes which can be located with an antibody against the CD18 molecule. In the present study, subtyping and cell counting were carried out on histological sections stained with Sirius red for eosinophils and with toluidine blue for mast cells. The CD18(+) cells were identified immunohistologically. Eosinophils and mast cells contributed considerably to the CD18(+) pool. The number of eosinophils in the corpus luteum increased rapidly in early development to approximately 90% of the CD18(+) cells, and decreased to 30% during secretion and to 10% during regression. Mast cells were not detectable in the follicles, the corpus luteum and the periphery of the cortex, but were observed in the interstitial cortical stroma and the medulla. The number of mast cells in these regions, which corresponded to 60-76% of the CD18(+) cells, did not change significantly throughout the oestrous cycle. It is concluded that eosinophils are selectively recruited at the periovulatory period and that mast cells are unevenly distributed.

Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow

“…The development of the bovine CL is accompanied by active angiogenesis, and the structure of blood vessels is retained during pregnancy (O'Shea et al 1989, Lei et al 1991, Beindorff et al 2010. Leukocytes, including monocytes/macrophages, granulocytes, and T lymphocytes, are localized to the CL in several species (Standaert et al 1991, Brannstrom et al 1994, Reibiger & Spanel-Borowski 2000. These findings suggest that the bovine CL should have a lymphatic system with essential roles in regulating the functions of its immune cells.…”

Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period

“…Leukocytes are attracted into the ovary and influence cyclically changing structures [1–3]. Recently, we reported that the eosinophils suddenly increase in number to roughly 90% of the leukocytes in the bovine corpus luteum of early development [4]. In sheep, the eosinophils are assumed to influence the neovascularization of the corpus luteum [5].…”

Mastócitos no lobo ventral da próstata, no epidídimo e no testículo de ratos UChB (consumidores voluntários de etanol a 10%)

“…15 Mastócitos foram extensivamente estudados em fêmeas, relacionando-os com as diversas fases do ciclo estral. 16,17,18,19 Diferentemente das fêmeas, há poucos trabalhos relacionando os mastócitos e o sistema reprodutor masculino. Gaytan et al 20 verificaram aumento na quantidade de mastócitos testiculares em ratos após tratamento neonatal com estrógeno, sem diferenças significativas na próstata e no epidídimo.…”