Difference in Subcellular Distribution of Mevalonate Pyrophosphate Decarboxylase Occurs by Cell Type

Abstract

Recently, it has been questioned whether mevalonate pyrophosphate decarboxylase (MPD) is predominantly located in the peroxisomes or cytosol. We previously reported that MPD was predominantly present in the cytosol of rat hepatocytes, normal rat kidney cells, or mouse melanoma cells. In the present study, we examined whether MPD was predominantly present in the cytosol of HepG2 (human hepatoma) cells and Cos7 (monkey kidney) cells using digitonin permeabilization. In HepG2 cells permeabilized with digitonin, 90 % and 10 % of MPD existed in the cytosol and membrane/organelle (M/O) fraction, respectively, while in Cos7 cells permeabilized with digitonin, 20 % and 80 % of MPD existed in the cytosol and M/O fraction, respectively. These data suggest that the difference in subcellular distribution of MPD is due to the cell type.

Comparison of the Gene Expression Levels of Mevalonate Pyrophosphate Decarboxylase between Stroke-prone Spontaneously Hhypertensive and Wistar Kyoto Rats

“…However, there is little information on the gene expression of MPD in rats, although analyses of the MPD protein have been performed. [6][7][8][9][10][11][12][13] Thus, we examined the distribution of the mRNA levels in Wistar rat tissues by real-time PCR, to clarify the relationship between protein and mRNA levels of MPD. Next, we compared the mRNA levels of MPD in liver and brain between SHRSP and normotensive WKY.…”