Difference in the attachment of hepatocytes between a poly(γ-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer cast surface and a PBLG/PNIPAAm Langmuir-Blodgett one

Shimojo, Shojiro; Akaike, Toshihiro; Hara, Mariko; Higuchi, Akon; Park, In Kyu; Cho, Chong Su

doi:10.1163/156856202760197447

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Our results were in good agreement with them. The cause might be that, both of CTS (Ikinci et al, 2002;Cho et al, 2004) and PNIPAAm (Shimojo et al, 2002;Cho et al, 2004) were reported to be biocompatible and could be employed as tissue engineering materials. Therefore, the resultant nanogels made of CTS and PNIPAAM retained their original effects to cells in some way.…”

Section: Cytotoxicity Evaluation Of Cross-linked Cts-g-pnipaam Nanogelsmentioning

confidence: 99%

Investigation of dual-sensitive nanogels based on chitosan andN-isopropylacrylamide and its intelligent drug delivery of 10-hydroxycamptothecine

Wang

et al. 2014

Drug Delivery

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The work was to prepare and characterize a responsive drug delivery system built of chitosan-g-poly (N-isopropylacrylamide) (CTS-g-PNIPAAm) nanogels and to evaluate the effects of CTS molecular weight (Mw) on the loading and in vitro release of insoluble drug 10-hydroxycamptothecine (HCPT). The CTS-g-PNIPAAm copolymers were synthesized by radical polymerization. The Mw and physical chemistry properties such as diameter, second virial coefficient of grafted PNIPAAm were investigated by dynamic and static laser light scattering method. A series of cross-linked CTS-g-PNIPAAm nanogels were prepared with N, N'-methylenebisacrylamide initially added as a cross-linker. The thermal and pH-sensitive features of cross-linked CTS-g-PNIPAAm nanogels were studied by determining the variance of transmittance, changeable size and reversed zeta potential. The critical aggregation concentrations (CAC) of resultant nanogels decreased from 0.045 to 0.036 mg/mL with CTS Mw increased from 50 kDa to 700 kDa. The loading efficiency of the HCPT encapsulated into CTS-g-PNIPAAm nanogels increased in parallel with CTS Mw, while the cumulative release percentage of HCPT-loaded nanogels decreased with CTS Mw increasing at both 25 °C and 37 °C. Fitting results of HCPT release data to different mathematical models suggested a diffusion-controlled mechanism at 25 °C. However, the release behaviors were dominated by combined effects of polymer erosion and osmotic pressure driven at 37 °C. The cytotoxicity study of the CTS-g-PNIPAAm nanogels against hepatic L02 cells indicated that the resultant nanogels did not exhibit apparent cytotoxicity.

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Section: Cytotoxicity Evaluation Of Cross-linked Cts-g-pnipaam Nanogelsmentioning

confidence: 99%

Investigation of dual-sensitive nanogels based on chitosan andN-isopropylacrylamide and its intelligent drug delivery of 10-hydroxycamptothecine

Wang

et al. 2014

Drug Delivery

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“…The literature data even scattered considerably, perhaps due to the complexity of the contact angle phenomenon as noted above. However, the sessile contact angle is very comparable to ∼75° of water on the LB surface of the diblock copolymer poly( γ -benzyl l -glutamate)- b -PNIPAM …”

Section: Resultsmentioning

confidence: 99%

Amphiphilic Gold Nanoparticles Grafted with Poly(N-isopropylacrylamide) and Polystyrene

et al. 2005

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Two types of amphiphilic gold nanoparticles (AuNP-1 and -2) grafted with a mixture of poly(N-isopropylacrylamide) (PNIPAM) and polystyrene (PS) chains in two different compositions have been successfully prepared with the "grafting-to" method in a homogeneous THF phase. These AuNPs were thoroughly characterized by FTIR, 1 H NMR, UV-vis, high-resolution transmission electron microscopy, thermogravimetric analysis, and dynamic light scattering to determine the total number of polymer chains bound to the gold nanoparticles, the ratio between PNIPAM and PS chains, and the size of the gold core. Langmuir monolayer experiments at the air-water interface of the two types of AuNPs revealed different compression isotherms of the surface pressure vs particle area (π-A curve) conducted at 20 °C. These amphiphilic gold nanoparticles can be regarded as analogues of amphiphilic diblock copolymers at the air-water interface. The compression isotherm of AuNP-2 with a PNIPAM:PS ratio of 2:1 showed several characteristic regions that can be attributed to the polymer conformational transitions from the pancake, the pancake to brush transition, to the brush. However, the monolayer of AuNP-1, with a ratio of 5:1 of PNIPAM:PS, never reaches a brush stage but showed an extension of the pseudoplateau region upon compression. These differences may be due to the more hydrophilic nature and the more stretched PNIPAM chains. Furthermore, the sessile drop contact angle measurements, conducted at room temperature on both upper and lower surfaces of the AuNP-2 monolayer transferred at 35 mN/m onto either hydrophilic or hydrophobic substrates, are slightly different, 82 ( 2°and 77 ( 2°, respectively. After comparing with the literature data of the contact angles of water on either the pure PS film or the PNIPAM brush, we concluded that the chemically different PNIPAM and PS chains grafted on the surface of the gold core tend to be phase-separated.

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Thermo-responsiveness of poly(-diethylacrylamide) polymers at the air–water interface: The effect of a hydrophobic block

Silva¹,

Lopes²,

Brogueira

et al. 2008

Journal of Colloid and Interface Science

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Difference in the attachment of hepatocytes between a poly(γ-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer cast surface and a PBLG/PNIPAAm Langmuir-Blodgett one

Cited by 3 publications

References 15 publications

Investigation of dual-sensitive nanogels based on chitosan andN-isopropylacrylamide and its intelligent drug delivery of 10-hydroxycamptothecine

Investigation of dual-sensitive nanogels based on chitosan andN-isopropylacrylamide and its intelligent drug delivery of 10-hydroxycamptothecine

Amphiphilic Gold Nanoparticles Grafted with Poly(N-isopropylacrylamide) and Polystyrene

Thermo-responsiveness of poly(-diethylacrylamide) polymers at the air–water interface: The effect of a hydrophobic block

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